Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro

Marisol Ocampo, Hernando Curtidor, Magnolia Vanegas, Manuel A. Patarroyo, Manuel E. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

6 Citas (Scopus)

Resumen

© 2014 John Wiley & Sons A/S.Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been based on proteins having immunogenic properties for patients suffering TB and having had promising results in mice and non-human primates. Our approach has been based on a functional approach involving the pathogen-host interaction in the search for antigens to be included in designing an efficient, minimal, subunit-based anti-TB vaccine. This means that Mycobacterium tuberculosis has mainly been involved in studies and that lipoproteins represent an important kind of protein on the cell envelope which can also contribute towards this pathogen's virulence. This study has assessed the expression of four lipoproteins from M. tuberculosis H37Rv, that is, Rv1411c (LprG), Rv1911c (LppC), Rv2270 (LppN) and Rv3763 (LpqH), and the possible biological activity of peptides derived from these. Five peptides were found for these proteins which had high specific binding to both alveolar A549 epithelial cells and U937 monocyte-derived macrophages which were able to significantly inhibit mycobacterial entry to these cells in vitro.
Idioma originalEnglish (US)
Páginas (desde-hasta)626-641
Número de páginas16
PublicaciónChemical Biology and Drug Design
Volumen84
N.º6
DOI
EstadoPublished - ene 1 2014

Huella dactilar

Mycobacterium tuberculosis
Tuberculosis Vaccines
Lipoproteins
Pathogens
Peptides
Tuberculosis
Vaccines
Alveolar Epithelial Cells
Host-Pathogen Interactions
Antigens
Proteins
Macrophages
Public health
Bioactivity
Nuclear Family
Primates
Virulence
Public Health
Mortality
In Vitro Techniques

Citar esto

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abstract = "{\circledC} 2014 John Wiley & Sons A/S.Tuberculosis (TB) continues being one of the diseases having the greatest mortality rates around the world, 8.7 million cases having been reported in 2011. An efficient vaccine against TB having a great impact on public health is an urgent need. Usually, selecting antigens for vaccines has been based on proteins having immunogenic properties for patients suffering TB and having had promising results in mice and non-human primates. Our approach has been based on a functional approach involving the pathogen-host interaction in the search for antigens to be included in designing an efficient, minimal, subunit-based anti-TB vaccine. This means that Mycobacterium tuberculosis has mainly been involved in studies and that lipoproteins represent an important kind of protein on the cell envelope which can also contribute towards this pathogen's virulence. This study has assessed the expression of four lipoproteins from M. tuberculosis H37Rv, that is, Rv1411c (LprG), Rv1911c (LppC), Rv2270 (LppN) and Rv3763 (LpqH), and the possible biological activity of peptides derived from these. Five peptides were found for these proteins which had high specific binding to both alveolar A549 epithelial cells and U937 monocyte-derived macrophages which were able to significantly inhibit mycobacterial entry to these cells in vitro.",
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Specific interaction between mycobacterium tuberculosis lipoprotein-derived peptides and target cells inhibits Mycobacterial entry in vitro. / Ocampo, Marisol; Curtidor, Hernando; Vanegas, Magnolia; Patarroyo, Manuel A.; Patarroyo, Manuel E.

En: Chemical Biology and Drug Design, Vol. 84, N.º 6, 01.01.2014, p. 626-641.

Resultado de la investigación: Contribución a RevistaArtículo

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AU - Ocampo, Marisol

AU - Curtidor, Hernando

AU - Vanegas, Magnolia

AU - Patarroyo, Manuel A.

AU - Patarroyo, Manuel E.

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