Specific erythrocyte binding capacity and biological activity of Plasmodium falciparum erythrocyte binding ligand 1 (EBL-1)-derived peptides

Hernando Curtidor, Luis E Rodríguez, Marisol Ocampo, Ramses López, Javier E García, John Valbuena, Ricardo Vera, Alvaro Puentes, Magnolia Vanegas, Manuel E Patarroyo

    Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

    12 Citas (Scopus)

    Resumen

    Erythrocyte binding ligand 1 (EBL-1) is a member of the ebl multigene family involved in Plasmodium falciparum invasion of erythrocytes. We found that five EBL-1 high-activity binding peptides (HABPs) bound specifically to erythrocytes: 29895 ((41)HKKKSGELNNNKSGILRSTY(60)), 29903 ((201)LYECGK-KIKEMKWICTDNQF(220)), 29923 ((601)CNAILGSYADIGDIVRGLDV(620)), 29924((621)WRDINTNKLSEK-FQKIFMGGY(640)), and 30018 ((2481)LEDIINLSKKKKKSINDTSFY(2500)). We also show that binding was saturable, not sialic acid-dependent, and that all peptides specifically bound to a 36-kDa protein on the erythrocyte membrane. The five HABPs inhibited in vitro merozoite invasion depending on the peptide concentration used, suggesting their possible role in the invasion process.

    Idioma originalInglés estadounidense
    Páginas (desde-hasta)464 - 473
    Número de páginas10
    PublicaciónProtein Science
    Volumen14
    N.º2
    DOI
    EstadoPublicada - feb. 2005

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