Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors

Luisa M. Arias-Giraldo; Marina Muñoz; Carolina Hernández; Giovanny Herrera; Natalia Velásquez-Ortiz; Omar Cantillo-Barraza; Plutarco Urbano; Juan David Ramírez

doi:10.1371/journal.pone.0240916

Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors

Título traducido de la contribución: Variación dependiente de la especie de las comunidades bacterianas del intestino en los insectos vectores de Trypanosoma cruzi

Luisa M. Arias-Giraldo, Marina Muñoz, Carolina Hernández, Giovanny Herrera, Natalia Velásquez-Ortiz, Omar Cantillo-Barraza, Plutarco Urbano, Juan David Ramírez

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5 Citas (Scopus)

Resumen

Los triatominos (Hemiptera: Reduviidae) son los insectos vectores de Trypanosoma cruzi, el agente causante de la enfermedad de Chagas. Las comunidades bacterianas del intestino afectan al desarrollo de T. cruzi dentro del vector, por lo que la caracterización de su composición es importante para entender el desarrollo de la infección. Se recolectaron 54 triatominos correspondientes a cuatro géneros en diferentes departamentos de Colombia. Se realizaron extracciones de ADN y PCR para evaluar la presencia de T. cruzi y determinar la unidad de tipificación discreta (DTU) del parásito. Los productos de PCR del gen 16S rRNA bacteriano se agruparon y secuenciaron. Las lecturas resultantes se desnaturalizaron y se utilizó QIIME 2 para la identificación de las variantes de la secuencia del amplicón (ASV). También se realizaron análisis de diversidad (diversidad alfa y beta) y de riqueza, gráficos Circos y análisis de componentes principales (PCA). La frecuencia global de infección por T. cruzi fue del 75,9%, siendo la TcI la DTU predominante. Se analizaron aproximadamente 500.000 secuencias y se identificaron 27 filos bacterianos. Los filos más abundantes fueron Proteobacterias (33,9%), Actinobacterias (32,4%), Firmicutes (19,6%) y Bacteroidetes (7,6%), que en conjunto representaron más del 90% de las comunidades intestinales identificadas en este estudio. Se identificaron los géneros de estos principales filos bacterianos, revelando la presencia de bacterias importantes como Rhodococcus, Serratia y Wolbachia. La composición de los filos bacterianos en el intestino de los insectos fue significativamente diferente entre las especies de triatominos, mientras que no se observaron diferencias significativas entre el estado de infección por T. cruzi. Sugerimos que se realicen más investigaciones con la evaluación de variables adicionales y un mayor tamaño de la muestra. Hasta donde sabemos, este estudio es la primera caracterización de la estructura bacteriana intestinal de los principales géneros de triatominos en Colombia.

Título traducido de la contribución	Variación dependiente de la especie de las comunidades bacterianas del intestino en los insectos vectores de Trypanosoma cruzi
Idioma original	Inglés estadounidense
Número de artículo	e0240916
Publicación	PLOS ONE
Volumen	15
N.º	11
DOI	https://doi.org/10.1371/journal.pone.0240916
Estado	Publicada - nov. 12 2020

Áreas temáticas de ASJC Scopus

Bioquímica, Genética y Biología Molecular General
Ciencias Agrícolas y Biológicas General
General

ODS de las Naciones Unidas

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title = "Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors",

abstract = "Triatomines (Hemiptera: Reduviidae) are the insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. The gut bacterial communities affect the development of T. cruzi inside the vector, making the characterization of its composition important in the understanding of infection development. We collected 54 triatomine bugs corresponding to four genera in different departments of Colombia. DNA extraction and PCR were performed to evaluate T. cruzi presence and to determine the discrete typing unit (DTU) of the parasite. PCR products of the bacterial 16S rRNA gene were pooled and sequenced. Resulting reads were denoised and QIIME 2 was used for the identification of amplicon sequence variants (ASVs). Diversity (alpha and beta diversity) and richness analyses, Circos plots, and principal component analysis (PCA) were also performed. The overall T. cruzi infection frequency was 75.9%, with TcI being the predominant DTU. Approximately 500,000 sequences were analyzed and 27 bacterial phyla were identified. The most abundant phyla were Proteobacteria (33.9%), Actinobacteria (32.4%), Firmicutes (19.6%), and Bacteroidetes (7.6%), which together accounted for over 90% of the gut communities identified in this study. Genera were identified for these main bacterial phyla, revealing the presence of important bacteria such as Rhodococcus, Serratia, and Wolbachia. The composition of bacterial phyla in the gut of the insects was significantly different between triatomine species, whereas no significant difference was seen between the state of T. cruzi infection. We suggest further investigation with the evaluation of additional variables and a larger sample size. To our knowledge, this study is the first characterization of the gut bacterial structure of the main triatomine genera in Colombia.",

author = "Arias-Giraldo, {Luisa M.} and Marina Mu{\~n}oz and Carolina Hern{\'a}ndez and Giovanny Herrera and Natalia Vel{\'a}squez-Ortiz and Omar Cantillo-Barraza and Plutarco Urbano and Ram{\'i}rez, {Juan David}",

note = "Funding Information: Funding: This work was funded by Direccio ´n de Investigacio ´ n e Innovacio ´ n from Universidad del Rosario.Thefundershadnoroleinstudydesign, datacollectionandanalysis,decisiontopublish,or preparationofthemanuscript. Publisher Copyright: Copyright: {\textcopyright} 2020 Arias-Giraldo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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AU - Arias-Giraldo, Luisa M.

AU - Muñoz, Marina

AU - Hernández, Carolina

AU - Herrera, Giovanny

AU - Velásquez-Ortiz, Natalia

AU - Cantillo-Barraza, Omar

AU - Urbano, Plutarco

AU - Ramírez, Juan David

N1 - Funding Information: Funding: This work was funded by Direccio ´n de Investigacio ´ n e Innovacio ´ n from Universidad del Rosario.Thefundershadnoroleinstudydesign, datacollectionandanalysis,decisiontopublish,or preparationofthemanuscript. Publisher Copyright: Copyright: © 2020 Arias-Giraldo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/12

Y1 - 2020/11/12

N2 - Triatomines (Hemiptera: Reduviidae) are the insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. The gut bacterial communities affect the development of T. cruzi inside the vector, making the characterization of its composition important in the understanding of infection development. We collected 54 triatomine bugs corresponding to four genera in different departments of Colombia. DNA extraction and PCR were performed to evaluate T. cruzi presence and to determine the discrete typing unit (DTU) of the parasite. PCR products of the bacterial 16S rRNA gene were pooled and sequenced. Resulting reads were denoised and QIIME 2 was used for the identification of amplicon sequence variants (ASVs). Diversity (alpha and beta diversity) and richness analyses, Circos plots, and principal component analysis (PCA) were also performed. The overall T. cruzi infection frequency was 75.9%, with TcI being the predominant DTU. Approximately 500,000 sequences were analyzed and 27 bacterial phyla were identified. The most abundant phyla were Proteobacteria (33.9%), Actinobacteria (32.4%), Firmicutes (19.6%), and Bacteroidetes (7.6%), which together accounted for over 90% of the gut communities identified in this study. Genera were identified for these main bacterial phyla, revealing the presence of important bacteria such as Rhodococcus, Serratia, and Wolbachia. The composition of bacterial phyla in the gut of the insects was significantly different between triatomine species, whereas no significant difference was seen between the state of T. cruzi infection. We suggest further investigation with the evaluation of additional variables and a larger sample size. To our knowledge, this study is the first characterization of the gut bacterial structure of the main triatomine genera in Colombia.

AB - Triatomines (Hemiptera: Reduviidae) are the insect vectors of Trypanosoma cruzi, the causative agent of Chagas disease. The gut bacterial communities affect the development of T. cruzi inside the vector, making the characterization of its composition important in the understanding of infection development. We collected 54 triatomine bugs corresponding to four genera in different departments of Colombia. DNA extraction and PCR were performed to evaluate T. cruzi presence and to determine the discrete typing unit (DTU) of the parasite. PCR products of the bacterial 16S rRNA gene were pooled and sequenced. Resulting reads were denoised and QIIME 2 was used for the identification of amplicon sequence variants (ASVs). Diversity (alpha and beta diversity) and richness analyses, Circos plots, and principal component analysis (PCA) were also performed. The overall T. cruzi infection frequency was 75.9%, with TcI being the predominant DTU. Approximately 500,000 sequences were analyzed and 27 bacterial phyla were identified. The most abundant phyla were Proteobacteria (33.9%), Actinobacteria (32.4%), Firmicutes (19.6%), and Bacteroidetes (7.6%), which together accounted for over 90% of the gut communities identified in this study. Genera were identified for these main bacterial phyla, revealing the presence of important bacteria such as Rhodococcus, Serratia, and Wolbachia. The composition of bacterial phyla in the gut of the insects was significantly different between triatomine species, whereas no significant difference was seen between the state of T. cruzi infection. We suggest further investigation with the evaluation of additional variables and a larger sample size. To our knowledge, this study is the first characterization of the gut bacterial structure of the main triatomine genera in Colombia.

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Species-dependent variation of the gut bacterial communities across Trypanosoma cruzi insect vectors

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