Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

Luisa Fernanda García-Salazar; Jean Alex Matos Ribeiro; Jonathan Emanuel Cunha; Stela Marcia Mattiello; Thiago Luiz Russo

doi:10.1080/10749357.2021.1956048

Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

Título traducido de la contribución: La actividad sérica de las metaloproteinasas de matriz-2 y -9 está aumentada en individuos con post-accidente cerebrovascular crónico: un estudio exploratorio transversal

Luisa Fernanda García-Salazar, Jean Alex Matos Ribeiro, Jonathan Emanuel Cunha, Stela Marcia Mattiello, Thiago Luiz Russo

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

5 Citas (Scopus)

Resumen

Antecedentes: Las concentraciones elevadas de metaloproteinasa de matriz-2 (MMP-2) y -9 (MMP-9) se asocian con la inflamación poco después del ictus. En etapas crónicas, la elevación de algunas citocinas se relaciona con la presencia de condiciones comórbidas en estos individuos. Además, algunas características como el deterioro sensoriomotor, la atrofia y el sedentarismo predisponen al sistema a una respuesta inflamatoria. Objetivo: Cuantificar la actividad sérica de MMP-2 y -9 en individuos post-ictus crónico y correlacionarla con variables de nivel de actividad física, composición corporal, capacidad funcional y de marcha, y con biomarcadores inflamatorios. Además, se caracterizó la actividad gelatinasa en función del deterioro motor. Métodos: Catorce pacientes con ictus de inicio >6 meses y siete individuos sanos fueron incluidos en este estudio. La evaluación clínica incluyó: composición corporal, medida mediante analizador de impedancia bioeléctrica; escala de evaluación motora de Fugl-Meyer; pruebas de marcha de seis minutos y diez metros, y nivel de actividad física evaluado mediante el monitor de actividad StepWatch®. Se recogieron muestras de sangre de la vena antecubital y se analizó la actividad de las MMP-2 y -9 séricas mediante zimografía en gelatina, y los biomarcadores TNFα, IL-6, IL-1β, IL-10 mediante kits ELISA. Resultados: Los individuos crónicos post-ictus presentaron una mayor actividad de MMP-2 y -9 en comparación con los individuos sanos. Se observaron correlaciones positivas con el tiempo y los pasos en cadencia baja y negativas con la cadencia media y el índice de actividad máxima. Según el deterioro motor, la actividad de la MMP-2 estaba aumentada en el grupo leve-moderado en comparación con el grupo control. Conclusiones: El aumento de gelatinasas en individuos crónicos post-ictus podría describir un proceso inflamatorio relacionado con la limitada capacidad de caminar a altas intensidades.

Título traducido de la contribución	La actividad sérica de las metaloproteinasas de matriz-2 y -9 está aumentada en individuos con post-accidente cerebrovascular crónico: un estudio exploratorio transversal
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	605-615
Número de páginas	10
Publicación	Topics in Stroke Rehabilitation
Volumen	29
N.º	8
DOI	https://doi.org/10.1080/10749357.2021.1956048
Estado	Publicación electrónica previa a su impresión - ago. 2 2021

Áreas temáticas de ASJC Scopus

Rehabilitación
Cuidados comunitarios y atención domiciliaria
Neurología clínica

ODS de las Naciones Unidas

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@article{53655e74782140859803e02a2da09c9d,

title = "Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study",

abstract = "Background: High concentrations of matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective: To quantify MMP-2 and −9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods: Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch{\textregistered} Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and −9 activity was analyzed using gelatin Zymography, and the TNFα, IL-6, IL-1β, IL-10 biomarkers using ELISA kits. Results: Chronic post-stroke individuals presented an increased activity of MMP-2 and −9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion: Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities. ",

author = "Garc{\'i}a-Salazar, {Luisa Fernanda} and Ribeiro, {Jean Alex Matos} and Cunha, {Jonathan Emanuel} and Mattiello, {Stela Marcia} and {Luiz Russo}, Thiago",

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year = "2021",

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day = "2",

doi = "10.1080/10749357.2021.1956048",

language = "English (US)",

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pages = "605--615",

journal = "Topics in Stroke Rehabilitation",

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Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study. / García-Salazar, Luisa Fernanda; Ribeiro, Jean Alex Matos; Cunha, Jonathan Emanuel et al.
En: Topics in Stroke Rehabilitation, Vol. 29, N.º 8, 02.08.2021, p. 605-615.

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals

T2 - a cross-sectional exploratory study

AU - García-Salazar, Luisa Fernanda

AU - Ribeiro, Jean Alex Matos

AU - Cunha, Jonathan Emanuel

AU - Mattiello, Stela Marcia

AU - Luiz Russo, Thiago

PY - 2021/8/2

Y1 - 2021/8/2

N2 - Background: High concentrations of matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective: To quantify MMP-2 and −9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods: Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch® Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and −9 activity was analyzed using gelatin Zymography, and the TNFα, IL-6, IL-1β, IL-10 biomarkers using ELISA kits. Results: Chronic post-stroke individuals presented an increased activity of MMP-2 and −9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion: Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities.

AB - Background: High concentrations of matrix metalloproteinase-2 (MMP-2) and −9 (MMP-9) are associated with inflammation early after stroke. In chronic stages, the elevation of some cytokines is related to the presence of co-morbid conditions in these individuals. In addition to this, some characteristics such as sensorimotor impairment, atrophy, and sedentary lifestyle predispose the system to an inflammatory response. Objective: To quantify MMP-2 and −9 serum activity in chronic post-stroke individuals and correlate it with variables of physical activity level, body composition, functional and walking capacity, and with inflammatory biomarkers. Additionally, gelatinase activity was characterized according to motor impairment. Methods: Fourteen patients with stroke onset >6 months and seven healthy individuals were enrolled in this study. The clinical assessment included: body composition, measure by bioelectrical impedance analyzer; Fugl-Meyer Motor Assessment Scale; six-minute and ten-meter walk tests, and physical activity level assessed by the StepWatch® Activity Monitor. Blood samples were collected from antecubital vein and serum MMP-2 and −9 activity was analyzed using gelatin Zymography, and the TNFα, IL-6, IL-1β, IL-10 biomarkers using ELISA kits. Results: Chronic post-stroke individuals presented an increased activity of MMP-2 and −9 compared to healthy individuals. Positive correlations with time and steps in low cadence and negative ones with medium cadence and peak activity index were observed. According to the motor impairment, the MMP-2 activity was increased in the mild-moderate group compared to the control group. Conclusion: Increased gelatinases in chronic post-stroke individuals could describe an inflammation process related to the limited capacity of walking in high intensities.

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DO - 10.1080/10749357.2021.1956048

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AN - SCOPUS:85111933789

SN - 1074-9357

VL - 29

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JO - Topics in Stroke Rehabilitation

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Serum activity of matrix metalloproteinase-2 and -9 is increased in chronic post-stroke individuals: a cross-sectional exploratory study

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