Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Stéphane Morisset; François Peyron; Jean R. Lobry; Justus Garweg; Josette Ferrandiz; Karine Musset; Jorge Enrique Gomez-Marin; Alejandra de la Torre; Magali Demar; Bernard Carme; Corinne Mercier; Jean François Garin; Marie France Cesbron-Delauw

doi:10.1016/j.micinf.2008.04.001

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Título traducido de la contribución: Serotipado de Toxoplasma gondii: sorprendente patrón homogéneo entre las infecciones sintomáticas y asintomáticas en Europa y América del Sur

Stéphane Morisset, François Peyron, Jean R. Lobry, Justus Garweg, Josette Ferrandiz, Karine Musset, Jorge Enrique Gomez-Marin, Alejandra de la Torre, Magali Demar, Bernard Carme, Corinne Mercier, Jean François Garin, Marie France Cesbron-Delauw

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

50 Citas (SciVal)

Resumen

Los aislados de campo de Toxoplasma gondii en Europa y América del Norte se han agrupado en tres linajes clonales que muestran diferente virulencia en ratones. Aún no se ha demostrado si la estructura genética del parásito está relacionada con la expresión clínica en humanos. Desarrollamos un ensayo inmunoabsorbente ligado a enzimas que utiliza polipéptidos polimórficos específicos del linaje derivados de los antígenos de gránulos densos, GRA5 y GRA6. Nuestro objetivo fue comparar los patrones serotípicos observados en pacientes asintomáticos versus sintomáticos (enfermedad ocular e infección grave en pacientes con VIH positivo) entre pacientes de Europa y América del Sur. Independientemente de la presentación clínica de la enfermedad, los serotipos difirieron según el origen geográfico, con una distribución homogénea del serotipo II en Europa y de los serotipos I y III en América del Sur. Concluimos que la serotipificación GRA5-GRA6 es una herramienta interesante para estudiar la prevalencia de serotipos en poblaciones, pero no es un marcador preciso de la patogenicidad de la infección por Toxoplasma en humanos.

Título traducido de la contribución	Serotipado de Toxoplasma gondii: sorprendente patrón homogéneo entre las infecciones sintomáticas y asintomáticas en Europa y América del Sur
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	742-747
Número de páginas	6
Publicación	Microbes and Infection
DOI	https://doi.org/10.1016/j.micinf.2008.04.001
Estado	Publicada - jun. 1 2008
Publicado de forma externa	Sí

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

Acceder al documento

10.1016/j.micinf.2008.04.001

Citar esto

Morisset, S., Peyron, F., Lobry, J. R., Garweg, J., Ferrandiz, J., Musset, K., Gomez-Marin, J. E., de la Torre, A., Demar, M., Carme, B., Mercier, C., Garin, J. F., & Cesbron-Delauw, M. F. (2008). Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America. Microbes and Infection, 742-747. https://doi.org/10.1016/j.micinf.2008.04.001

@article{b214dc05ee8f4298a6ba45361ac8d6b4,

title = "Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America",

abstract = "Field isolates of Toxoplasma gondii in Europe and North America have been grouped into three clonal lineages that display different virulence in mice. Whether the genetic structure of the parasite is related to clinical expression in humans has not yet been demonstrated. We developed an enzyme-linked immunosorbent assay which uses lineage-specific, polymorphic polypeptides derived from the dense granule antigens, GRA5 and GRA6. Our goal was to compare serotypical patterns observed in asymptomatic versus symptomatic (ocular disease and severe infection in human immunodeficiency virus (HIV)-positive patients) infections among patients from Europe and South America. Independent of the clinical presentation of the disease, serotypes differed according to geographical origin, with a homogeneous distribution of serotype II in Europe and of serotypes I and III in South America. We conclude that GRA5-GRA6 serotyping is an interesting tool to study serotype prevalence in populations but it is not an accurate marker of pathogenicity of Toxoplasma infection in humans. ",

author = "St{\'e}phane Morisset and Fran{\c c}ois Peyron and Lobry, {Jean R.} and Justus Garweg and Josette Ferrandiz and Karine Musset and Gomez-Marin, {Jorge Enrique} and {de la Torre}, Alejandra and Magali Demar and Bernard Carme and Corinne Mercier and Garin, {Jean Fran{\c c}ois} and Cesbron-Delauw, {Marie France}",

year = "2008",

month = jun,

day = "1",

doi = "10.1016/j.micinf.2008.04.001",

language = "English (US)",

pages = "742--747",

journal = "Microbes and Infection",

issn = "1286-4579",

publisher = "Elsevier Masson SAS",

}

Morisset, S, Peyron, F, Lobry, JR, Garweg, J, Ferrandiz, J, Musset, K, Gomez-Marin, JE, de la Torre, A, Demar, M, Carme, B, Mercier, C, Garin, JF & Cesbron-Delauw, MF 2008, 'Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America', Microbes and Infection, pp. 742-747. https://doi.org/10.1016/j.micinf.2008.04.001

TY - JOUR

T1 - Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

AU - Morisset, Stéphane

AU - Peyron, François

AU - Lobry, Jean R.

AU - Garweg, Justus

AU - Ferrandiz, Josette

AU - Musset, Karine

AU - Gomez-Marin, Jorge Enrique

AU - de la Torre, Alejandra

AU - Demar, Magali

AU - Carme, Bernard

AU - Mercier, Corinne

AU - Garin, Jean François

AU - Cesbron-Delauw, Marie France

PY - 2008/6/1

Y1 - 2008/6/1

N2 - Field isolates of Toxoplasma gondii in Europe and North America have been grouped into three clonal lineages that display different virulence in mice. Whether the genetic structure of the parasite is related to clinical expression in humans has not yet been demonstrated. We developed an enzyme-linked immunosorbent assay which uses lineage-specific, polymorphic polypeptides derived from the dense granule antigens, GRA5 and GRA6. Our goal was to compare serotypical patterns observed in asymptomatic versus symptomatic (ocular disease and severe infection in human immunodeficiency virus (HIV)-positive patients) infections among patients from Europe and South America. Independent of the clinical presentation of the disease, serotypes differed according to geographical origin, with a homogeneous distribution of serotype II in Europe and of serotypes I and III in South America. We conclude that GRA5-GRA6 serotyping is an interesting tool to study serotype prevalence in populations but it is not an accurate marker of pathogenicity of Toxoplasma infection in humans.

AB - Field isolates of Toxoplasma gondii in Europe and North America have been grouped into three clonal lineages that display different virulence in mice. Whether the genetic structure of the parasite is related to clinical expression in humans has not yet been demonstrated. We developed an enzyme-linked immunosorbent assay which uses lineage-specific, polymorphic polypeptides derived from the dense granule antigens, GRA5 and GRA6. Our goal was to compare serotypical patterns observed in asymptomatic versus symptomatic (ocular disease and severe infection in human immunodeficiency virus (HIV)-positive patients) infections among patients from Europe and South America. Independent of the clinical presentation of the disease, serotypes differed according to geographical origin, with a homogeneous distribution of serotype II in Europe and of serotypes I and III in South America. We conclude that GRA5-GRA6 serotyping is an interesting tool to study serotype prevalence in populations but it is not an accurate marker of pathogenicity of Toxoplasma infection in humans.

U2 - 10.1016/j.micinf.2008.04.001

DO - 10.1016/j.micinf.2008.04.001

M3 - Article

SN - 1286-4579

SP - 742

EP - 747

JO - Microbes and Infection

JF - Microbes and Infection

ER -

Serotyping of Toxoplasma gondii: striking homogeneous pattern between symptomatic and asymptomatic infections within Europe and South America

Resumen

ODS de las Naciones Unidas

Acceder al documento

Huella

Citar esto