Sequences of the Plasmodium falciparum cytoadherence-linked asexual protein 9 implicated in malaria parasite invasion to erythrocytes

Carlos Giovanni Pinzón, Hernando Curtidor, Jeison García, Magnolia Vanegas, Carolina Vizcaíno, Manuel A. Patarroyo, Manuel E. Patarroyo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

7 Citas (Scopus)

Resumen

In this study, we synthesized the complete sequence of the CLAG-9 protein as 67 20-mer-long non-overlapped peptides and assessed their ability to bind to erythrocytes in receptor-ligand assays. Twenty CLAG-9 peptides were found to have specific high-affinity binding ability to erythrocytes (thereby named as HABPs), with nanomolar dissociation constants. CLAG-9 HABPs interacted with different erythrocyte surface receptors having apparent molecular weights of 85, 63 and 34 kDa. CLAG-9 HABPs binding was also affected by pre-treatment of RBCs with enzymes and inhibited erythrocyte invasion in vitro by up to 72% at 200 μM. These results suggest that some protein fragments of CLAG-9 may be part of the molecular machinery used by malaria parasites to invade erythrocytes, hence supporting their study as possible vaccine candidates.

Idioma originalInglés estadounidense
Páginas (desde-hasta)2653-2663
Número de páginas11
PublicaciónVaccine
Volumen28
N.º14
DOI
EstadoPublicada - mar. 19 2010

Áreas temáticas de ASJC Scopus

  • Medicina molecular
  • Inmunología y Microbiología General
  • Veterinaria General
  • Salud pública, medioambiental y laboral
  • Enfermedades infecciosas

Huella

Profundice en los temas de investigación de 'Sequences of the Plasmodium falciparum cytoadherence-linked asexual protein 9 implicated in malaria parasite invasion to erythrocytes'. En conjunto forman una huella única.

Citar esto