Sarconesin

Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

Título traducido de la contribución: Sarconesin : Sarconesiopsis magellanica Excreciones y Secreciones Larvarias con Propiedades Antibacterianas

Andrea Díaz-Roa, Manuel A Patarroyo, Felio J Bello, Pedro I Da Silva

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

La terapia larvaria (LT) es un tratamiento alternativo para curar heridas crónicas; su acción se basa en el desbridamiento, la eliminación de bacterias y la estimulación del tejido de granulación. El mecanismo más importante cuando se utiliza la LT para combatir la infección depende de las excreciones y secreciones larvarias (ES). Las larvas están protegidas contra la infección por un espectro de péptidos antimicrobianos (PAM); también ha aumentado el interés especial por los PAM en cuanto a la comprensión de su papel en la cicatrización de heridas, ya que degradan el tejido necrótico y matan diferentes bacterias durante la LT. La Sarconesiopsis magellanica (Diptera: Calliphoridae) es una prometedora mosca necrófaga de importancia médica. Este artículo reporta un pequeño AMP aislado de los productos de S. magellanica ES por primera vez; estos productos fueron obtenidos de larvas de tercer estadio tomadas de una colonia previamente establecida. ES fueron fraccionados por RP-HPLC utilizando columnas C18 para el primer análisis; los productos fueron luego liofilizados y su actividad antimicrobiana se caracterizó por la incubación con diferentes cepas bacterianas. Las secuencias primarias de estas fracciones se determinaron por espectrometría de masas y secuenciación de novo; se obtuvieron cinco AMPs, se caracterizó la fracción de Sarconesina y se probó la actividad antibacteriana en diferentes concentraciones con concentraciones inhibitorias mínimas a partir de 1.2 μM Se demostró una potente actividad inhibitoria contra las bacterias Gram-negativas (Escherichia coli D31, E. coli α, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) y Gram-positivas (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270). La sarconesina tiene una similitud significativa con las GTPasas de la familia Rho, que son importantes en el desarrollo de organelos, la dinámica citoesquelética, el movimiento celular y la reparación de heridas. Los datos reportados aquí indican que la Sarconesina podría ser un candidato alternativo para su uso en terapéutica contra infecciones bacterianas Gram-negativas y Gram-positivas. Nuestro estudio describe un péptido responsable de la actividad antibacteriana cuando se utiliza LT. Los resultados mostrados aquí apoyan la realización de nuevos experimentos destinados a validar los AMPs de S. magellanica como nuevos recursos para combatir la resistencia antibacteriana.
Idioma originalEnglish (US)
Páginas (desde-hasta)2249
PublicaciónFrontiers in Microbiology
Volumen9
DOI
EstadoPublished - sep 28 2018
Publicado de forma externa

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title = "Sarconesin: Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties",
abstract = "Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions' primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 μM. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5α, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance.",
author = "Andrea D{\'i}az-Roa and Patarroyo, {Manuel A} and Bello, {Felio J} and {Da Silva}, {Pedro I}",
year = "2018",
month = "9",
day = "28",
doi = "10.3389/fmicb.2018.02249",
language = "English (US)",
volume = "9",
pages = "2249",
journal = "Frontiers in Microbiology",
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Sarconesin : Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties. / Díaz-Roa, Andrea; Patarroyo, Manuel A; Bello, Felio J; Da Silva, Pedro I.

En: Frontiers in Microbiology, Vol. 9, 28.09.2018, p. 2249.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Sarconesin

T2 - Sarconesiopsis magellanica Blowfly Larval Excretions and Secretions With Antibacterial Properties

AU - Díaz-Roa, Andrea

AU - Patarroyo, Manuel A

AU - Bello, Felio J

AU - Da Silva, Pedro I

PY - 2018/9/28

Y1 - 2018/9/28

N2 - Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions' primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 μM. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5α, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance.

AB - Larval therapy (LT) is an alternative treatment for healing chronic wounds; its action is based on debridement, the removal of bacteria, and stimulating granulation tissue. The most important mechanism when using LT for combating infection depends on larval excretions and secretions (ES). Larvae are protected against infection by a spectrum of antimicrobial peptides (AMPs); special interest in AMPs has also risen regarding understanding their role in wound healing since they degrade necrotic tissue and kill different bacteria during LT. Sarconesiopsis magellanica (Diptera: Calliphoridae) is a promising medically-important necrophagous fly. This article reports a small AMP being isolated from S. magellanica ES products for the first time; these products were obtained from third-instar larvae taken from a previously-established colony. ES were fractionated by RP-HPLC using C18 columns for the first analysis; the products were then lyophilised and their antimicrobial activity was characterized by incubation with different bacterial strains. These fractions' primary sequences were determined by mass spectrometry and de novo sequencing; five AMPs were obtained, the Sarconesin fraction was characterized and antibacterial activity was tested in different concentrations with minimum inhibitory concentrations starting at 1.2 μM. Potent inhibitory activity was shown against Gram-negative (Escherichia coli D31, E. coli DH5α, Salmonella enterica ATCC 13314, Pseudomonas aeruginosa 27853) and Gram-positive (Staphylococcus aureus ATCC 29213, S. epidermidis ATCC 12228, Micrococcus luteus A270) bacteria. Sarconesin has a significant similarity with Rho-family GTPases which are important in organelle development, cytoskeletal dynamics, cell movement, and wound repair. The data reported here indicated that Sarconesin could be an alternative candidate for use in therapeutics against Gram-negative and Gram-positive bacterial infections. Our study describes one peptide responsible for antibacterial activity when LT is being used. The results shown here support carrying out further experiments aimed at validating S. magellanica AMPs as novel resources for combating antibacterial resistance.

U2 - 10.3389/fmicb.2018.02249

DO - 10.3389/fmicb.2018.02249

M3 - Article

VL - 9

SP - 2249

JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

ER -