Rv1268c protein peptide inhibiting Mycobacterium tuberculosis H37Rv entry to target cells

Marisol Ocampo, Deisy Carolina Rodríguez, Jorge Rodríguez, Maritza Bermúdez, Claudia Marina Muñoz, Manuel Alfonso Patarroyo, Manuel Elkin Patarroyo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

5 Citas (Scopus)

Resumen

Tuberculosis (TB) remains one of the most worrying infectious diseases affecting public health around the world; 8.7 million new TB cases were reported in 2011. The search for an Mycobacterium tuberculosis H37Rv protein sequence which is functionally important in host-pathogen interaction has been proposed for developing a new vaccine which will allow efficient and safe control of the spread of this disease. The present study thus reports the results obtained for the Rv1268c protein described in the M. tuberculosis H37Rv genome as a hypothetical unknown, probably secreted, protein based on a highly robust, specific, sensitive and functional approach to the search for potential epitopes to be included in an anti-tuberculosis vaccine. Rv1268c presence was determined by immunoblotting after obtaining polyclonal sera against mycobacterial total sonicate or subcellular fractions. Such sera were used in electron immunomicroscopy (EIM) for confirming protein localisation on the M. tuberculosis envelop by recognising colloidal gold-labelled immunoglobulin. Screening assays revealed the presence of two sequences having high binding activity: one binding A549 alveolar epithelial cells ( 141TGMAALEQYLGSGHAVIVSI160) and other binding U937 monocyte-derived macrophages (21AVALGLASPADAAAGTMYGD40). Such sequences' ability to inhibit mycobacterial entry during in vitro assays was analysed. The structure of synthetic peptides binding to target cells was also determined, bearing in mind the structure-function relationship. These results, together with those obtained for other proteins, have been involved in selecting peptides which might be included in a subunit-based anti-tuberculosis vaccine.

Idioma originalInglés estadounidense
Páginas (desde-hasta)6650-6656
Número de páginas7
PublicaciónBioorganic and Medicinal Chemistry
Volumen21
N.º21
DOI
EstadoPublicada - nov. 1 2013

Áreas temáticas de ASJC Scopus

  • Bioquímica
  • Medicina molecular
  • Biología molecular
  • Ciencias farmacéuticas
  • Descubrimiento de medicamentos
  • Bioquímica clínica
  • Química orgánica

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