PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis

Luis A. Baquero, Darwin A. Moreno-Pérez, Diego Garzón-Ospina, Johanna Forero-Rodríguez, Heidy D. Ortiz-Suárez, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

9 Citas (Scopus)

Resumen

Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells.

Idioma originalEnglish (US)
Número de artículo251
PublicaciónParasites and Vectors
Volumen10
N.º1
DOI
EstadoPublished - may 19 2017
Publicado de forma externa

Huella dactilar

Plasmodium vivax
Genetic Selection
Reticulocytes
Antigens
Schizonts
Merozoites
Plasmodium
Cell Adhesion
Parasites
Proteins

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Infectious Diseases

Citar esto

Baquero, L. A., Moreno-Pérez, D. A., Garzón-Ospina, D., Forero-Rodríguez, J., Ortiz-Suárez, H. D., & Patarroyo, M. A. (2017). PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis. Parasites and Vectors, 10(1), [251]. https://doi.org/10.1186/s13071-017-2183-8
Baquero, Luis A. ; Moreno-Pérez, Darwin A. ; Garzón-Ospina, Diego ; Forero-Rodríguez, Johanna ; Ortiz-Suárez, Heidy D. ; Patarroyo, Manuel A. / PvGAMA reticulocyte binding activity : predicting conserved functional regions by natural selection analysis. En: Parasites and Vectors. 2017 ; Vol. 10, N.º 1.
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abstract = "Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells.",
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Baquero, LA, Moreno-Pérez, DA, Garzón-Ospina, D, Forero-Rodríguez, J, Ortiz-Suárez, HD & Patarroyo, MA 2017, 'PvGAMA reticulocyte binding activity: predicting conserved functional regions by natural selection analysis', Parasites and Vectors, vol. 10, n.º 1, 251. https://doi.org/10.1186/s13071-017-2183-8

PvGAMA reticulocyte binding activity : predicting conserved functional regions by natural selection analysis. / Baquero, Luis A.; Moreno-Pérez, Darwin A.; Garzón-Ospina, Diego; Forero-Rodríguez, Johanna; Ortiz-Suárez, Heidy D.; Patarroyo, Manuel A.

En: Parasites and Vectors, Vol. 10, N.º 1, 251, 19.05.2017.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - PvGAMA reticulocyte binding activity

T2 - predicting conserved functional regions by natural selection analysis

AU - Baquero, Luis A.

AU - Moreno-Pérez, Darwin A.

AU - Garzón-Ospina, Diego

AU - Forero-Rodríguez, Johanna

AU - Ortiz-Suárez, Heidy D.

AU - Patarroyo, Manuel A.

PY - 2017/5/19

Y1 - 2017/5/19

N2 - Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells.

AB - Background: Adhesin proteins are used by Plasmodium parasites to bind and invade target cells. Hence, characterising molecules that participate in reticulocyte interaction is key to understanding the molecular basis of Plasmodium vivax invasion. This study focused on predicting functionally restricted regions of the P. vivax GPI-anchored micronemal antigen (PvGAMA) and characterising their reticulocyte binding activity. Results: The pvgama gene was initially found in P. vivax VCG-I strain schizonts. According to the genetic diversity analysis, PvGAMA displayed a size polymorphism very common for antigenic P. vivax proteins. Two regions along the antigen sequence were highly conserved among species, having a negative natural selection signal. Interestingly, these regions revealed a functional role regarding preferential target cell adhesion. Conclusions: To our knowledge, this study describes PvGAMA reticulocyte binding properties for the first time. Conserved functional regions were predicted according to natural selection analysis and their binding ability was confirmed. These findings support the notion that PvGAMA may have an important role in P. vivax merozoite adhesion to its target cells.

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