Proteolytic hydrolysis and purification of the LRP/alfa-2-macroglobulin receptor domain from α-macroglobulins

Daniel Iván Barrera, Luisa Marina Matheus, Torgny Stigbrand, Luis Fernando Arbeláez

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

5 Citas (Scopus)

Resumen

A new, easier and efficient purification method, using Sephacryl and DEAE-Sephacel, of the C-terminal fragment of two α-macroglobulins, α2-M and PZP, is presented. Two larger peptides were identified for each protein as the C-terminal fragment, with molecular weights of ∼30 kDa and the N-terminal sequences were determined to be SSTQDTV for α2-M and VALHLS for PZP. The smaller peptides with molecular weights of 18 kDa correspond to a shorter C-terminal sequence of these proteins, and they were determined to be EEFPFA for α2-M and ALKVQTV for PZP, with no interfering sequences detected. The results confirmed the discriminatory capacity of the purification procedure and the purity of the fragments. This new methodology facilitates biological studies of α-macroglobulins, and will enable elucidation of the role the C-terminal region may exert to eliminate α-macroglobulin-proteinases complexes from the circulation by the LRP/receptor.

Idioma originalInglés estadounidense
Páginas (desde-hasta)112-118
Número de páginas7
PublicaciónProtein Expression and Purification
Volumen53
N.º1
DOI
EstadoPublicada - may 2007

All Science Journal Classification (ASJC) codes

  • Biotecnología

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