Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys

Juan Carlos Polanco, Josefa A. Antonia Rodr????guez, Vladimir Corredor, Manuel Alfonso Patarroyo

    Resultado de la investigación: Contribución a libro /Tipo informe o reporteCapítulo

    16 Citas (Scopus)

    Resumen

    Plasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. We present data validating a real-time PCR assay quantifying P. vivax parasitemia, using the small subunit ribosomal RNA genes as an amplification target. P. vivax species-specific primers were designed on the 18S ribosomal gene V8 region, for amplifying both asexual and sporozoite ssrRNA genes. The assay detects amplification products bound to fluorescent SYBR-Green I dye using Perkin-Elmer GeneAmp-5700-SDS. Linear range standard curves from 6 DNA concentration logs (+0.99 correlation coefficients) were obtained. Standard curves were constructed using a plasmid containing target gene for real-time PCR amplification. This P. vivax specific assay is very sensitive, having a three parasite detection limit, and is reproducible and accurate. It involves a "closed-tube" PCR, avoids time-consuming post-PCR manipulation, and decreases potential PCR contamination. ?? 2002 Elsevier Science (USA). All rights reserved.
    Idioma originalEnglish (US)
    Título de la publicación alojadaExperimental Parasitology
    Páginas131-134
    Número de páginas4
    DOI
    EstadoPublished - feb 1 2002

    Serie de la publicación

    NombreExperimental Parasitology
    Volumen100

    Huella dactilar

    Plasmodium vivax
    Parasitemia
    Haplorhini
    Real-Time Polymerase Chain Reaction
    Polymerase Chain Reaction
    Genes
    Sporozoites
    Gene Amplification
    Plasmodium falciparum
    rRNA Genes
    Malaria
    Limit of Detection
    Parasites
    Plasmids
    Coloring Agents
    DNA

    Citar esto

    Polanco, J. C., Antonia Rodr????guez, J. A., Corredor, V., & Patarroyo, M. A. (2002). Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys. En Experimental Parasitology (pp. 131-134). (Experimental Parasitology; Vol. 100). https://doi.org/10.1016/S0014-4894(02)00010-3
    Polanco, Juan Carlos ; Antonia Rodr????guez, Josefa A. ; Corredor, Vladimir ; Patarroyo, Manuel Alfonso. / Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys. Experimental Parasitology. 2002. pp. 131-134 (Experimental Parasitology).
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    abstract = "Plasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. We present data validating a real-time PCR assay quantifying P. vivax parasitemia, using the small subunit ribosomal RNA genes as an amplification target. P. vivax species-specific primers were designed on the 18S ribosomal gene V8 region, for amplifying both asexual and sporozoite ssrRNA genes. The assay detects amplification products bound to fluorescent SYBR-Green I dye using Perkin-Elmer GeneAmp-5700-SDS. Linear range standard curves from 6 DNA concentration logs (+0.99 correlation coefficients) were obtained. Standard curves were constructed using a plasmid containing target gene for real-time PCR amplification. This P. vivax specific assay is very sensitive, having a three parasite detection limit, and is reproducible and accurate. It involves a {"}closed-tube{"} PCR, avoids time-consuming post-PCR manipulation, and decreases potential PCR contamination. ?? 2002 Elsevier Science (USA). All rights reserved.",
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    Polanco, JC, Antonia Rodr????guez, JA, Corredor, V & Patarroyo, MA 2002, Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys. En Experimental Parasitology. Experimental Parasitology, vol. 100, pp. 131-134. https://doi.org/10.1016/S0014-4894(02)00010-3

    Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys. / Polanco, Juan Carlos; Antonia Rodr????guez, Josefa A.; Corredor, Vladimir; Patarroyo, Manuel Alfonso.

    Experimental Parasitology. 2002. p. 131-134 (Experimental Parasitology; Vol. 100).

    Resultado de la investigación: Contribución a libro /Tipo informe o reporteCapítulo

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    T1 - Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys

    AU - Polanco, Juan Carlos

    AU - Antonia Rodr????guez, Josefa A.

    AU - Corredor, Vladimir

    AU - Patarroyo, Manuel Alfonso

    PY - 2002/2/1

    Y1 - 2002/2/1

    N2 - Plasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. We present data validating a real-time PCR assay quantifying P. vivax parasitemia, using the small subunit ribosomal RNA genes as an amplification target. P. vivax species-specific primers were designed on the 18S ribosomal gene V8 region, for amplifying both asexual and sporozoite ssrRNA genes. The assay detects amplification products bound to fluorescent SYBR-Green I dye using Perkin-Elmer GeneAmp-5700-SDS. Linear range standard curves from 6 DNA concentration logs (+0.99 correlation coefficients) were obtained. Standard curves were constructed using a plasmid containing target gene for real-time PCR amplification. This P. vivax specific assay is very sensitive, having a three parasite detection limit, and is reproducible and accurate. It involves a "closed-tube" PCR, avoids time-consuming post-PCR manipulation, and decreases potential PCR contamination. ?? 2002 Elsevier Science (USA). All rights reserved.

    AB - Plasmodium vivax and Plasmodium falciparum are the two prevalent human malaria species. A Colombian P. vivax wild strain has been adapted in Aotus nancymaae monkeys for use in further biological and immunological studies. We present data validating a real-time PCR assay quantifying P. vivax parasitemia, using the small subunit ribosomal RNA genes as an amplification target. P. vivax species-specific primers were designed on the 18S ribosomal gene V8 region, for amplifying both asexual and sporozoite ssrRNA genes. The assay detects amplification products bound to fluorescent SYBR-Green I dye using Perkin-Elmer GeneAmp-5700-SDS. Linear range standard curves from 6 DNA concentration logs (+0.99 correlation coefficients) were obtained. Standard curves were constructed using a plasmid containing target gene for real-time PCR amplification. This P. vivax specific assay is very sensitive, having a three parasite detection limit, and is reproducible and accurate. It involves a "closed-tube" PCR, avoids time-consuming post-PCR manipulation, and decreases potential PCR contamination. ?? 2002 Elsevier Science (USA). All rights reserved.

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    Polanco JC, Antonia Rodr????guez JA, Corredor V, Patarroyo MA. Plasmodium vivax: Parasitemia determination by real-time quantitative PCR in Aotus monkeys. En Experimental Parasitology. 2002. p. 131-134. (Experimental Parasitology). https://doi.org/10.1016/S0014-4894(02)00010-3