Plasmodium vivax in vitro continuous culture: the spoke in the wheel

Maritza Bermúdez, Darwin Andrés Moreno-Pérez, Gabriela Arévalo-Pinzón, Hernando Curtidor, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo de revisión

2 Citas (Scopus)

Resumen

Comprender el ciclo de vida de Plasmodium vivax es fundamental para desarrollar estrategias dirigidas a controlar y eliminar esta especie parasitaria. Aunque los avances en las ciencias ómicas y las técnicas de alto rendimiento de los últimos años han permitido la identificación y caracterización de proteínas que podrían estar participando en la invasión de las células diana por P. vivax, el tropismo parasitario exclusivo de los reticulocitos invasores se ha convertido en el principal obstáculo para mantener un cultivo continuo de esta especie. Tal avance que ayudaría a definir la función de cada proteína del parásito en el complejo proceso de invasión de P. vivax, además de evaluar nuevos agentes terapéuticos, sigue siendo un sueño. Se han realizado avances relacionados con el mantenimiento, los suplementos de medios de cultivo y el uso de diferentes fuentes de reticulocitos y parásitos (cepas y aislados) en el desarrollo de un cultivo in vitro de P. vivax; sin embargo, hasta la fecha sólo se han obtenido algunos cultivos con pocos ciclos de replicación, lo que significa que el mantenimiento de este parásito va más allá de los componentes técnicos implicados. Aunque todavía no está claro qué mecanismos moleculares prefiere P. vivax para invadir los reticulocitos jóvenes CD71+[etapas tempranas de maduración (I-II-III)], los cambios relacionados con la remodelación de las proteínas de la membrana de dichas células podrían formar parte de la explicación. En esta revisión se han analizado los aspectos más relevantes relacionados con el cultivo in vitro de P. vivax y las características de las células huéspedes para explicar las posibles razones por las que el cultivo in vitro continuo de la especie es tan difícil de estandarizar. También se han descrito algunas alternativas para el cultivo in vitro de P. vivax.
Idioma originalEnglish (US)
Páginas (desde-hasta)301
PublicaciónMalaria Journal
Volumen17
N.º1
DOI
EstadoPublished - ago 20 2018

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Bermúdez, Maritza ; Moreno-Pérez, Darwin Andrés ; Arévalo-Pinzón, Gabriela ; Curtidor, Hernando ; Patarroyo, Manuel Alfonso. / Plasmodium vivax in vitro continuous culture : the spoke in the wheel. En: Malaria Journal. 2018 ; Vol. 17, N.º 1. pp. 301.
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title = "Plasmodium vivax in vitro continuous culture: the spoke in the wheel",
abstract = "Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described.",
author = "Maritza Berm{\'u}dez and Moreno-P{\'e}rez, {Darwin Andr{\'e}s} and Gabriela Ar{\'e}valo-Pinz{\'o}n and Hernando Curtidor and Patarroyo, {Manuel Alfonso}",
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Plasmodium vivax in vitro continuous culture : the spoke in the wheel. / Bermúdez, Maritza; Moreno-Pérez, Darwin Andrés; Arévalo-Pinzón, Gabriela; Curtidor, Hernando; Patarroyo, Manuel Alfonso.

En: Malaria Journal, Vol. 17, N.º 1, 20.08.2018, p. 301.

Resultado de la investigación: Contribución a RevistaArtículo de revisión

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AU - Bermúdez, Maritza

AU - Moreno-Pérez, Darwin Andrés

AU - Arévalo-Pinzón, Gabriela

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AU - Patarroyo, Manuel Alfonso

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N2 - Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described.

AB - Understanding the life cycle of Plasmodium vivax is fundamental for developing strategies aimed at controlling and eliminating this parasitic species. Although advances in omic sciences and high-throughput techniques in recent years have enabled the identification and characterization of proteins which might be participating in P. vivax invasion of target cells, exclusive parasite tropism for invading reticulocytes has become the main obstacle in maintaining a continuous culture for this species. Such advance that would help in defining each parasite protein's function in the complex process of P. vivax invasion, in addition to evaluating new therapeutic agents, is still a dream. Advances related to maintenance, culture medium supplements and the use of different sources of reticulocytes and parasites (strains and isolates) have been made regarding the development of an in vitro culture for P. vivax; however, only some cultures having few replication cycles have been obtained to date, meaning that this parasite's maintenance goes beyond the technical components involved. Although it is still not yet clear which molecular mechanisms P. vivax prefers for invading young CD71+ reticulocytes [early maturation stages (I-II-III)], changes related to membrane proteins remodelling of such cells could form part of the explanation. The most relevant aspects regarding P. vivax in vitro culture and host cell characteristics have been analysed in this review to explain possible reasons why the species' continuous in vitro culture is so difficult to standardize. Some alternatives for P. vivax in vitro culture have also been described.

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