Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect

Carlos Hernando Niño, Juan Ricardo Cubides, Paola Andrea Camargo-Ayala, Carlos Arturo Rodríguez-Celis, Teódulo Quiñones, Moisés Tomás Cortés-Castillo, Lizeth Sánchez-Suárez, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

    Resultado de la investigación: Contribución a RevistaArtículo

    7 Citas (Scopus)

    Resumen

    © 2016 The Author(s).Background: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department. Methods: A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia's Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. Conclusion: The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted.
    Idioma originalEnglish (US)
    PublicaciónMalaria Journal
    DOI
    EstadoPublished - nov 29 2016

    Huella dactilar

    Plasmodium malariae
    Malaria
    Plasmodium
    Colombia
    Polymerase Chain Reaction
    Hematologic Tests
    Parasites
    Vivax Malaria
    Endemic Diseases
    Asymptomatic Infections
    Phlebotomy
    Parasitemia
    Plasmodium falciparum
    Coinfection
    Routine Diagnostic Tests
    Public Health
    Cross-Sectional Studies
    Infection

    Citar esto

    Niño, C. H., Cubides, J. R., Camargo-Ayala, P. A., Rodríguez-Celis, C. A., Quiñones, T., Cortés-Castillo, M. T., ... Patarroyo, M. A. (2016). Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect. Malaria Journal. https://doi.org/10.1186/s12936-016-1629-3
    Niño, Carlos Hernando ; Cubides, Juan Ricardo ; Camargo-Ayala, Paola Andrea ; Rodríguez-Celis, Carlos Arturo ; Quiñones, Teódulo ; Cortés-Castillo, Moisés Tomás ; Sánchez-Suárez, Lizeth ; Sánchez, Ricardo ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect. En: Malaria Journal. 2016.
    @article{d8ae5f3c26a448d1b22a3f99672c472e,
    title = "Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect",
    abstract = "{\circledC} 2016 The Author(s).Background: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department. Methods: A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia's Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. Conclusion: The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted.",
    author = "Ni{\~n}o, {Carlos Hernando} and Cubides, {Juan Ricardo} and Camargo-Ayala, {Paola Andrea} and Rodr{\'i}guez-Celis, {Carlos Arturo} and Te{\'o}dulo Qui{\~n}ones and Cort{\'e}s-Castillo, {Mois{\'e}s Tom{\'a}s} and Lizeth S{\'a}nchez-Su{\'a}rez and Ricardo S{\'a}nchez and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
    year = "2016",
    month = "11",
    day = "29",
    doi = "10.1186/s12936-016-1629-3",
    language = "English (US)",
    journal = "Malaria Journal",
    issn = "1475-2875",
    publisher = "BioMed Central",

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    Niño, CH, Cubides, JR, Camargo-Ayala, PA, Rodríguez-Celis, CA, Quiñones, T, Cortés-Castillo, MT, Sánchez-Suárez, L, Sánchez, R, Patarroyo, ME & Patarroyo, MA 2016, 'Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect', Malaria Journal. https://doi.org/10.1186/s12936-016-1629-3

    Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect. / Niño, Carlos Hernando; Cubides, Juan Ricardo; Camargo-Ayala, Paola Andrea; Rodríguez-Celis, Carlos Arturo; Quiñones, Teódulo; Cortés-Castillo, Moisés Tomás; Sánchez-Suárez, Lizeth; Sánchez, Ricardo; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

    En: Malaria Journal, 29.11.2016.

    Resultado de la investigación: Contribución a RevistaArtículo

    TY - JOUR

    T1 - Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect

    AU - Niño, Carlos Hernando

    AU - Cubides, Juan Ricardo

    AU - Camargo-Ayala, Paola Andrea

    AU - Rodríguez-Celis, Carlos Arturo

    AU - Quiñones, Teódulo

    AU - Cortés-Castillo, Moisés Tomás

    AU - Sánchez-Suárez, Lizeth

    AU - Sánchez, Ricardo

    AU - Patarroyo, Manuel Elkin

    AU - Patarroyo, Manuel Alfonso

    PY - 2016/11/29

    Y1 - 2016/11/29

    N2 - © 2016 The Author(s).Background: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department. Methods: A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia's Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. Conclusion: The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted.

    AB - © 2016 The Author(s).Background: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department. Methods: A total of 1392 blood samples (taken by venepuncture) from symptomatic patients in Colombia's Amazon department were analysed in parallel by thick blood smear (TBS) test and nested PCR for determining Plasmodium spp. infection and identifying infecting species, such as Plasmodium vivax, Plasmodium malariae and/or Plasmodium falciparum. Descriptive statistics were used for comparing the results from both tests regarding detection of the disease, typing infecting species and their prevalence in the study region. Bearing the microscope assay in mind as gold standard, PCR diagnosis performance was evaluated by statistical indicators. Conclusion: The present study revealed great differences between both diagnostic tests, as well as suggesting high P. malariae prevalence from a molecular perspective. This differed profoundly from previous studies in this region of Colombia, usually based on the TBS test, suggesting that diagnosis by conventional techniques could lead to underestimating the prevalence of certain Plasmodium spp. having high circulation in this area. The present results highlight the need for modifying state malaria surveillance schemes for more efficient strategies regarding the detection of this disease in endemic areas. The importance of PCR as a back-up test in cases of low parasitaemia or mixed infection is also highlighted.

    U2 - 10.1186/s12936-016-1629-3

    DO - 10.1186/s12936-016-1629-3

    M3 - Article

    JO - Malaria Journal

    JF - Malaria Journal

    SN - 1475-2875

    ER -

    Niño CH, Cubides JR, Camargo-Ayala PA, Rodríguez-Celis CA, Quiñones T, Cortés-Castillo MT y otros. Plasmodium malariae in the Colombian Amazon region: You don't diagnose what you don't suspect. Malaria Journal. 2016 nov 29. https://doi.org/10.1186/s12936-016-1629-3