Occurrence of blastocystis in patients with clostridioides difficile infection

Laura Vega; Giovanny Herrera; Marina Muñoz; Manuel Alfonso Patarroyo; Juan David Ramírez

doi:10.3390/pathogens9040283

Occurrence of blastocystis in patients with clostridioides difficile infection

Título traducido de la contribución: Aparición de blastocystis en pacientes con infección por clostridioides difficile

Laura Vega, Giovanny Herrera, Marina Muñoz, Manuel Alfonso Patarroyo, Juan David Ramírez

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

13 Citas (Scopus)

Resumen

Clostridiodes difficile constituye una amenaza para la salud pública que ha sido poco estudiada en Colombia. Las cepas hipervirulentas de C. difficile albergan múltiples toxinas, pueden diseminarse fácilmente y pueden provocar la aparición de la enfermedad dentro de los centros sanitarios (HCFO) y la comunidad (CO). Los estudios han demostrado que una microbiota alterada (por ejemplo, disbiosis) puede permitir la infección por C. difficile (CDI). Se ha sugerido que la disbiosis impide la colonización por el eucariota anaerobio Blastocystis, posiblemente debido a un aumento de la tensión luminal de oxígeno. Ningún estudio ha encontrado la co-ocurrencia de CDI y Blastocystis. Por lo tanto, nos propusimos determinar las frecuencias de infección/colonización por C. difficile y Blastocystis en 220 muestras fecales diarreicas. Se realizó la detección molecular por PCR de ambos microorganismos, con análisis descriptivos de cuatro variables (detección de CDI, determinación de perfiles toxigénicos de C. difficile, detección de Blastocystis y lugar de inicio del paciente). Demostramos una asociación significativa entre la presencia de Blastocystis y CDI, con coinfección encontrada en 61 pacientes, y mostramos una alta frecuencia de CDI entre los grupos de HCFO y CO. Nuestros resultados de las frecuencias de coinfección podrían apoyar las hipótesis que sugieren que Blastocystis puede adaptarse a la disbiosis y al estrés oxidativo. Además, la presencia de C. difficile toxigénico fuera de las instalaciones sanitarias que se muestra aquí hace saltar la alarma sobre la propagación en la comunidad.

Título traducido de la contribución	Aparición de blastocystis en pacientes con infección por clostridioides difficile
Idioma original	Inglés estadounidense
Número de artículo	283
Número de páginas	8
Publicación	Pathogens
Volumen	9
N.º	4
DOI	https://doi.org/10.3390/pathogens9040283
Estado	Publicada - abr. 2020

Áreas temáticas de ASJC Scopus

Inmulogía y alergología
Biología molecular
Inmunología y Microbiología General
Microbiología (médica)
Enfermedades infecciosas

ODS de las Naciones Unidas

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title = "Occurrence of blastocystis in patients with clostridioides difficile infection",

abstract = "Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread.",

author = "Laura Vega and Giovanny Herrera and Marina Mu{\~n}oz and Patarroyo, {Manuel Alfonso} and Ram{\'i}rez, {Juan David}",

note = "Funding Information: Funding: This research was funded by the Departamento Administrativo de Ciencia, Tecnolog{\'i}a e Innovaci{\'o}n (Colciencias) within the framework of the project “Determinaci{\'o}n del microbioma intestinal en pacientes con diarrea asociada a la infecci{\'o}n por Clostridioides difficile adquirida en Unidad de Cuidados Intensivos y comunidad” code 212477758147, contract number 606-2018, call 777 from 2017. Publisher Copyright: {\textcopyright} 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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AU - Muñoz, Marina

AU - Patarroyo, Manuel Alfonso

AU - Ramírez, Juan David

N1 - Funding Information: Funding: This research was funded by the Departamento Administrativo de Ciencia, Tecnología e Innovación (Colciencias) within the framework of the project “Determinación del microbioma intestinal en pacientes con diarrea asociada a la infección por Clostridioides difficile adquirida en Unidad de Cuidados Intensivos y comunidad” code 212477758147, contract number 606-2018, call 777 from 2017. Publisher Copyright: © 2020 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/4

Y1 - 2020/4

N2 - Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread.

AB - Clostridiodes difficile comprises a public-health threat that has been understudied in Colombia. Hypervirulent strains of C. difficile harbor multiple toxins, can be easily spread, and can have their onset of disease within healthcare facilities (HCFO) and the community (CO). Studies have shown that a disrupted microbiota (e.g., dysbiosis) may allow C. difficile infection (CDI). It has been suggested that dysbiosis prevents colonization by the anaerobic eukaryote Blastocystis, possibly due to an increase in luminal oxygen tension. No study has found co-occurrence of CDI and Blastocystis. Therefore, we aimed to determine the frequencies of C. difficile and Blastocystis infection/colonization in 220 diarrheal fecal samples. Molecular detection by PCR for both microorganisms was performed, with descriptive analyses of four variables (CDI detection, determination of C. difficile toxigenic profiles, Blastocystis detection, and patient site of onset). We demonstrate a significant association between the presence of Blastocystis and CDI, with coinfection found in 61 patients, and show a high frequency of CDI among both HCFO and CO groups. Our results of coinfection frequencies could support hypotheses that suggest Blastocystis can adapt to dysbiosis and oxidative stress. Further, the presence of toxigenic C. difficile occurring outside healthcare facilities shown here raises the alarm for community wide spread.

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Occurrence of blastocystis in patients with clostridioides difficile infection

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