Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

Diana Marcela Rodríguez, Marisol Ocampo, Hernando Curtidor, Magnolia Vanegas, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

7 Citas (Scopus)

Resumen

Mycobacterium tuberculosis surface proteins involved in target cell invasion may be identified as a strategy for developing subunit-based, chemically-synthesized vaccines. The Rv0227c protein was thus selected to assess its role in the invasion and infection of Mycobacterium tuberculosis target cells. Results revealed Rv0227c localization on mycobacterial surface by immunoelectron microscopy and Western blot. Receptor-ligand assays using 20-mer, non-overlapping peptides covering the complete Rv0227c protein sequence revealed three high activity binding peptides for U937 phagocytic cells and seven for A549 cells. Peptide 16944 significantly inhibited mycobacterial entry to both cell lines while 16943 and 16949 only managed to inhibit entrance to U937 cells and 16951 to A549 cells. The Jnet bioinformatics tool predicted secondary structure elements for the complete protein, agreeing with elements determined for such chemically-synthesized peptides. It was thus concluded that high activity binding peptides which were able to inhibit mycobacterial entry to target cells are of great importance when selecting peptide candidates for inclusion in an anti-tuberculosis vaccine. © 2012 Elsevier Inc.
Idioma originalEnglish (US)
Páginas (desde-hasta)208-216
Número de páginas9
PublicaciónPeptides
DOI
EstadoPublished - dic 1 2012

Huella dactilar

Mycobacterium tuberculosis
Membrane Proteins
Peptides
U937 Cells
Tuberculosis Vaccines
Proteins
Immunoelectron Microscopy
Bioinformatics
Phagocytes
Computational Biology
Assays
Microscopic examination
Vaccines
Western Blotting
Cells
Ligands
Cell Line
Infection

Citar esto

Rodríguez, Diana Marcela ; Ocampo, Marisol ; Curtidor, Hernando ; Vanegas, Magnolia ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion. En: Peptides. 2012 ; pp. 208-216.
@article{f9542e67cea2403c846fbdb38fbcd06f,
title = "Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion",
abstract = "Mycobacterium tuberculosis surface proteins involved in target cell invasion may be identified as a strategy for developing subunit-based, chemically-synthesized vaccines. The Rv0227c protein was thus selected to assess its role in the invasion and infection of Mycobacterium tuberculosis target cells. Results revealed Rv0227c localization on mycobacterial surface by immunoelectron microscopy and Western blot. Receptor-ligand assays using 20-mer, non-overlapping peptides covering the complete Rv0227c protein sequence revealed three high activity binding peptides for U937 phagocytic cells and seven for A549 cells. Peptide 16944 significantly inhibited mycobacterial entry to both cell lines while 16943 and 16949 only managed to inhibit entrance to U937 cells and 16951 to A549 cells. The Jnet bioinformatics tool predicted secondary structure elements for the complete protein, agreeing with elements determined for such chemically-synthesized peptides. It was thus concluded that high activity binding peptides which were able to inhibit mycobacterial entry to target cells are of great importance when selecting peptide candidates for inclusion in an anti-tuberculosis vaccine. {\circledC} 2012 Elsevier Inc.",
author = "Rodr{\'i}guez, {Diana Marcela} and Marisol Ocampo and Hernando Curtidor and Magnolia Vanegas and Patarroyo, {Manuel Elkin} and Patarroyo, {Manuel Alfonso}",
year = "2012",
month = "12",
day = "1",
doi = "10.1016/j.peptides.2012.08.023",
language = "English (US)",
pages = "208--216",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",

}

Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion. / Rodríguez, Diana Marcela; Ocampo, Marisol; Curtidor, Hernando; Vanegas, Magnolia; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

En: Peptides, 01.12.2012, p. 208-216.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

AU - Rodríguez, Diana Marcela

AU - Ocampo, Marisol

AU - Curtidor, Hernando

AU - Vanegas, Magnolia

AU - Patarroyo, Manuel Elkin

AU - Patarroyo, Manuel Alfonso

PY - 2012/12/1

Y1 - 2012/12/1

N2 - Mycobacterium tuberculosis surface proteins involved in target cell invasion may be identified as a strategy for developing subunit-based, chemically-synthesized vaccines. The Rv0227c protein was thus selected to assess its role in the invasion and infection of Mycobacterium tuberculosis target cells. Results revealed Rv0227c localization on mycobacterial surface by immunoelectron microscopy and Western blot. Receptor-ligand assays using 20-mer, non-overlapping peptides covering the complete Rv0227c protein sequence revealed three high activity binding peptides for U937 phagocytic cells and seven for A549 cells. Peptide 16944 significantly inhibited mycobacterial entry to both cell lines while 16943 and 16949 only managed to inhibit entrance to U937 cells and 16951 to A549 cells. The Jnet bioinformatics tool predicted secondary structure elements for the complete protein, agreeing with elements determined for such chemically-synthesized peptides. It was thus concluded that high activity binding peptides which were able to inhibit mycobacterial entry to target cells are of great importance when selecting peptide candidates for inclusion in an anti-tuberculosis vaccine. © 2012 Elsevier Inc.

AB - Mycobacterium tuberculosis surface proteins involved in target cell invasion may be identified as a strategy for developing subunit-based, chemically-synthesized vaccines. The Rv0227c protein was thus selected to assess its role in the invasion and infection of Mycobacterium tuberculosis target cells. Results revealed Rv0227c localization on mycobacterial surface by immunoelectron microscopy and Western blot. Receptor-ligand assays using 20-mer, non-overlapping peptides covering the complete Rv0227c protein sequence revealed three high activity binding peptides for U937 phagocytic cells and seven for A549 cells. Peptide 16944 significantly inhibited mycobacterial entry to both cell lines while 16943 and 16949 only managed to inhibit entrance to U937 cells and 16951 to A549 cells. The Jnet bioinformatics tool predicted secondary structure elements for the complete protein, agreeing with elements determined for such chemically-synthesized peptides. It was thus concluded that high activity binding peptides which were able to inhibit mycobacterial entry to target cells are of great importance when selecting peptide candidates for inclusion in an anti-tuberculosis vaccine. © 2012 Elsevier Inc.

U2 - 10.1016/j.peptides.2012.08.023

DO - 10.1016/j.peptides.2012.08.023

M3 - Article

SP - 208

EP - 216

JO - Peptides

JF - Peptides

SN - 0196-9781

ER -