Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia

Jubby Marcela Gálvez, Henry Mauricio Chaparro-Solano, Ángela María Pinzón-Rondón, Ludwig L. Albornoz, Juan Mauricio Pardo-Oviedo, Fabio Andrés Zapata-Gómez, Andrés Felipe Patiño-Aldana, Andrea del Pila Hernández-Rodríguez, Mateo Díaz-Quiroz, Ángela María Ruiz-Sternberg

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is the etiopathogenic agent of COVID-19, a condition that has led to a formally recognized pandemic by March 2020 (World Health Organization –WHO). The SARS-CoV-2 genome is constituted of 29,903 base pairs, that code for four structural proteins (N, M, S, and E) and more than 20 non-structural proteins. Mutations in any of these regions, especially in those that encode for the structural proteins, have allowed the identification of diverse lineages around the world, some of them named as Variants of Concern (VOC) and Variants of Interest (VOI), according to the WHO and CDC. In this study, by using Next Generation Sequencing (NGS) technology, we sequenced the SARS-CoV-2 genome of 422 samples from Colombian residents, all of them collected between April 2020 and January 2021. We obtained genetic information from 386 samples, leading us to the identification of 14 new lineages circulating in Colombia, 13 of which were identified for the first time in South America. GH was the predominant GISAID clade in our sample. Most mutations were either missense (53.6%) or synonymous mutations (37.4%), and most genetic changes were located in the ORF1ab gene (63.9%), followed by the S gene (12.9%). In the latter, we identified mutations E484K, L18F, and D614G. Recent evidence suggests that these mutations concede important particularities to the virus, compromising host immunity, the diagnostic test performance, and the effectiveness of some vaccines. Some important lineages containing these mutations are the Alpha, Beta, and Gamma (WHO Label). Further genomic surveillance is important for the understanding of emerging genomic variants and their correlation with disease severity.

Idioma originalInglés estadounidense
Número de artículo105192
Páginas (desde-hasta)1-8
Número de páginas8
PublicaciónInfection, Genetics and Evolution
Volumen97
DOI
EstadoPublicada - ene. 2022

Áreas temáticas de ASJC Scopus

  • Microbiología
  • Ecología, evolución, comportamiento y sistemática
  • Biología molecular
  • Genética
  • Microbiología (médica)
  • Enfermedades infecciosas

Palabras claves de autor

  • Concepto

Huella

Profundice en los temas de investigación de 'Mutation profile of SARS-CoV-2 genome in a sample from the first year of the pandemic in Colombia'. En conjunto forman una huella única.

Citar esto