Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis

Luis Asencios; Marco Galarza; Neyda Quispe; Lucy Vásquez; Elena Leo; Eddy Valencia; Juan Ramírez; Margoth Acurio; Rosario Salazar; Alberto Mendoza-Ticona; Omar Cáceres

doi:10.1590/S1726-46342012000100014

Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis

Título traducido de la contribución: Prueba molecular Genotype®MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente

Luis Asencios, Marco Galarza, Neyda Quispe, Lucy Vásquez, Elena Leo, Eddy Valencia, Juan Ramírez, Margoth Acurio, Rosario Salazar, Alberto Mendoza-Ticona, Omar Cáceres

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

24 Citas (Scopus)

Resumen

The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.

Título traducido de la contribución	Prueba molecular Genotype®MTBDRplus, una alternativa para la detección rápida de tuberculosis multidrogorresistente
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	92-98
Número de páginas	7
Publicación	Revista Peruana de Medicina Experimental y Salud Publica
Volumen	29
N.º	1
DOI	https://doi.org/10.1590/S1726-46342012000100014
Estado	Publicada - mar. 2012
Publicado de forma externa	Sí

Áreas temáticas de ASJC Scopus

Salud pública, medioambiental y laboral

ODS de las Naciones Unidas

Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

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10.1590/S1726-46342012000100014

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Asencios, L., Galarza, M., Quispe, N., Vásquez, L., Leo, E., Valencia, E., Ramírez, J., Acurio, M., Salazar, R., Mendoza-Ticona, A., & Cáceres, O. (2012). Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis. Revista Peruana de Medicina Experimental y Salud Publica, 29(1), 92-98. https://doi.org/10.1590/S1726-46342012000100014

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abstract = "The Genotype{\textregistered}MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method {"}Agar Plate Proportions{"} (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype{\textregistered}MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.",

author = "Luis Asencios and Marco Galarza and Neyda Quispe and Lucy V{\'a}squez and Elena Leo and Eddy Valencia and Juan Ram{\'i}rez and Margoth Acurio and Rosario Salazar and Alberto Mendoza-Ticona and Omar C{\'a}ceres",

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Asencios, L, Galarza, M, Quispe, N, Vásquez, L, Leo, E, Valencia, E, Ramírez, J, Acurio, M, Salazar, R, Mendoza-Ticona, A & Cáceres, O 2012, 'Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis', Revista Peruana de Medicina Experimental y Salud Publica, vol. 29, n.º 1, pp. 92-98. https://doi.org/10.1590/S1726-46342012000100014

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AU - Asencios, Luis

AU - Galarza, Marco

AU - Quispe, Neyda

AU - Vásquez, Lucy

AU - Leo, Elena

AU - Valencia, Eddy

AU - Ramírez, Juan

AU - Acurio, Margoth

AU - Salazar, Rosario

AU - Mendoza-Ticona, Alberto

AU - Cáceres, Omar

PY - 2012/3

Y1 - 2012/3

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AB - The Genotype®MTBDRplus molecular test is a method that allows identifcation of the most frequent mutations associated with resistance to major frst-line antituberculosis drugs, Isoniazid (INH) and Rifampicin (RfP). The aim of this study was to evaluate the performance of the molecular test with culture and smear- positive sputum samples. We evaluated 95 cultures and 100 sputum samples with resistance profles previously determined by the reference method "Agar Plate Proportions" (APP). The molecular test from cultures showed a sensitivity of 100%, 97,5% and 96,97% for RIf, INH and MDR respectively while from sputums the sensitivity was 95,65%, 96,77% and 95,24% for RIf, INH and MDR respectively. We conclude that the molecular test Genotype®MTBDRplus is a very useful tool to detect resistance to isoniazid and rifampicin simultaneously (MDR-TB) in up to 72 hours from sputum samples or cultures.

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Molecular test Genotype®MTBDRplus, an alternative to rapid detection of multidrug resistance tuberculosis

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ODS de las Naciones Unidas

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