Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia

Bibiana Chavarro Portillo, Jaime Enrique Moreno, Nancy Yomayusa, Carlos Arturo Álvarez, Betsy Esperanza Castro Cardozo, Javier Antonio Escobar Pérez, Paula Lucia Díaz, Milciades Ibañez, Sebastián Mendez-Alvarez, Aura Lucia Leal, Natasha Vanegas Gómez

Resultado de la investigación: Contribución a RevistaArtículo

12 Citas (Scopus)

Resumen

Objective: To determine the molecular epidemiology and presence of virulence genes in community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCC. mec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p=0.001, odds ratio 4.73) and a protective effect for eta (p=0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCC. mec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. © 2013 International Society for Infectious Diseases.
Idioma originalEnglish (US)
Páginas (desde-hasta)e744-e749
Número de páginas6
PublicaciónInternational Journal of Infectious Diseases
Volumen17
N.º9
DOI
EstadoPublished - sep 1 2013
Publicado de forma externa

Huella dactilar

Colombia
Molecular Epidemiology
Community Hospital
Methicillin-Resistant Staphylococcus aureus
Virulence
Genes
Clone Cells
Infection
Odds Ratio
Multilocus Sequence Typing
Mortality
Pulsed Field Gel Electrophoresis
Enterotoxins
Multigene Family
Observational Studies
Multivariate Analysis
Prospective Studies

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Portillo, Bibiana Chavarro ; Moreno, Jaime Enrique ; Yomayusa, Nancy ; Álvarez, Carlos Arturo ; Cardozo, Betsy Esperanza Castro ; Pérez, Javier Antonio Escobar ; Díaz, Paula Lucia ; Ibañez, Milciades ; Mendez-Alvarez, Sebastián ; Leal, Aura Lucia ; Gómez, Natasha Vanegas. / Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia. En: International Journal of Infectious Diseases. 2013 ; Vol. 17, N.º 9. pp. e744-e749.
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title = "Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia",
abstract = "Objective: To determine the molecular epidemiology and presence of virulence genes in community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCC. mec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68{\%} of the isolates were classified as HA-MRSA and 32{\%} as CA-MRSA. We identified differences in the patterns of virulence genes: 85{\%} of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92{\%} of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p=0.001, odds ratio 4.73) and a protective effect for eta (p=0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCC. mec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. {\circledC} 2013 International Society for Infectious Diseases.",
author = "Portillo, {Bibiana Chavarro} and Moreno, {Jaime Enrique} and Nancy Yomayusa and {\'A}lvarez, {Carlos Arturo} and Cardozo, {Betsy Esperanza Castro} and P{\'e}rez, {Javier Antonio Escobar} and D{\'i}az, {Paula Lucia} and Milciades Iba{\~n}ez and Sebasti{\'a}n Mendez-Alvarez and Leal, {Aura Lucia} and G{\'o}mez, {Natasha Vanegas}",
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Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia. / Portillo, Bibiana Chavarro; Moreno, Jaime Enrique; Yomayusa, Nancy; Álvarez, Carlos Arturo; Cardozo, Betsy Esperanza Castro; Pérez, Javier Antonio Escobar; Díaz, Paula Lucia; Ibañez, Milciades; Mendez-Alvarez, Sebastián; Leal, Aura Lucia; Gómez, Natasha Vanegas.

En: International Journal of Infectious Diseases, Vol. 17, N.º 9, 01.09.2013, p. e744-e749.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Molecular epidemiology and characterization of virulence genes of community-acquired and hospital-acquired methicillin-resistant staphylococcus aureus isolates in Colombia

AU - Portillo, Bibiana Chavarro

AU - Moreno, Jaime Enrique

AU - Yomayusa, Nancy

AU - Álvarez, Carlos Arturo

AU - Cardozo, Betsy Esperanza Castro

AU - Pérez, Javier Antonio Escobar

AU - Díaz, Paula Lucia

AU - Ibañez, Milciades

AU - Mendez-Alvarez, Sebastián

AU - Leal, Aura Lucia

AU - Gómez, Natasha Vanegas

PY - 2013/9/1

Y1 - 2013/9/1

N2 - Objective: To determine the molecular epidemiology and presence of virulence genes in community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCC. mec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p=0.001, odds ratio 4.73) and a protective effect for eta (p=0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCC. mec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. © 2013 International Society for Infectious Diseases.

AB - Objective: To determine the molecular epidemiology and presence of virulence genes in community-acquired (CA) and hospital-acquired (HA) methicillin-resistant Staphylococcus aureus (MRSA) isolates and their relationship to clinical outcomes. Methods: An observational and prospective study of infections caused by MRSA was conducted between June 2006 and December 2007 across seven hospitals in three Colombian cities. MRSA isolates were analyzed for SCC. mec. Also, pulsed-field gel electrophoresis and multilocus sequence typing were performed and 25 virulence genes were identified. Results: Two hundred and seventy isolates were collected from 262 adult hospital patients with MRSA infections. Overall, 68% of the isolates were classified as HA-MRSA and 32% as CA-MRSA. We identified differences in the patterns of virulence genes: 85% of HA-MRSA isolates possessed the enterotoxin gene cluster (egc), whereas 92% of CA-MRSA isolates possessed the lukF-PV/lukS-PV genes. Multivariate analysis showed an increased risk of mortality for seg (p=0.001, odds ratio 4.73) and a protective effect for eta (p=0.018, odds ratio 0.33). Conclusions: Our study confirms that three clones of MRSA predominantly circulate in Colombia: a Chilean clone, a pediatric clone that causes HA-MRSA infections, and a USA300-related clone (SCC. mec IVc) in CA-MRSA infections, which differ in the content of clinically important virulence genes. This study confirms that PVL is not a determinant of severity or mortality in CA-MRSA infections. © 2013 International Society for Infectious Diseases.

U2 - 10.1016/j.ijid.2013.02.029

DO - 10.1016/j.ijid.2013.02.029

M3 - Article

VL - 17

SP - e744-e749

JO - International Journal of Infectious Diseases

JF - International Journal of Infectious Diseases

SN - 1201-9712

IS - 9

ER -