Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombia

Juan David Ramírez, Rubén Darío Heredia, Carolina Hernández, Cielo M. León, Ligia Inés Moncada, Patricia Reyes, Análida Elizabeth Pinilla, Myriam Consuelo Lopez

Resultado de la investigación: Contribución a RevistaArtículo

28 Citas (Scopus)

Resumen

© 2015 Elsevier B.V.Giardiasis is a parasitic infection that affects around 200 million people worldwide. This parasite presents a remarkable genetic variability observed in 8 genetic clusters named as 'assemblages' (A-H). These assemblages are host restricted and could be zoonotic where A and B infect humans and animals around the globe. The knowledge of the molecular epidemiology of human giardiasis in South-America is scarce and also the usefulness of PCR to detect this pathogen in fecal samples remains controversial. The aim of this study was to conduct a cross-sectional study to compare the molecular targets employed for the molecular diagnosis of Giardia DNA and to discriminate the parasite assemblages circulating in the studied population. We analyzed 181 fecal samples from Children at La Virgen, Cundinamarca, Colombia that were DNA-extracted and analyzed by SSU rDNA, tpi and gdh loci. We observed positivity by microscopy of 13% and by PCR around 76-80% depending on the molecular marker. Additionally, a lack of statistical concordance between microscopy and PCR was detected. Regarding the genetic assemblages, we detected assemblage A (3%), assemblage B (90%) and mixed infections assemblages A. +. B (7%). Hence, the sub-assemblages were typed as AI, AII, BIII and BIV across the population. This study represents a reliable attempt to understand the molecular epidemiology of giardiasis in Colombia and the use of PCR to detect cryptic infections. The epidemiological implications are herein discussed.
Idioma originalEnglish (US)
Páginas (desde-hasta)208-213
Número de páginas6
PublicaciónInfection, Genetics and Evolution
DOI
EstadoPublished - jun 1 2015

Huella dactilar

giardiasis
Giardia lamblia
Colombia
Giardiasis
rural areas
rural area
molecular epidemiology
genotype
Genotype
epidemiology
Polymerase Chain Reaction
Molecular Epidemiology
microscopy
parasite
DNA
parasites
Microscopy
Bovine Immunodeficiency Virus
Parasites
Giardia

Citar esto

Ramírez, Juan David ; Heredia, Rubén Darío ; Hernández, Carolina ; León, Cielo M. ; Moncada, Ligia Inés ; Reyes, Patricia ; Pinilla, Análida Elizabeth ; Lopez, Myriam Consuelo. / Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombia. En: Infection, Genetics and Evolution. 2015 ; pp. 208-213.
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abstract = "{\circledC} 2015 Elsevier B.V.Giardiasis is a parasitic infection that affects around 200 million people worldwide. This parasite presents a remarkable genetic variability observed in 8 genetic clusters named as 'assemblages' (A-H). These assemblages are host restricted and could be zoonotic where A and B infect humans and animals around the globe. The knowledge of the molecular epidemiology of human giardiasis in South-America is scarce and also the usefulness of PCR to detect this pathogen in fecal samples remains controversial. The aim of this study was to conduct a cross-sectional study to compare the molecular targets employed for the molecular diagnosis of Giardia DNA and to discriminate the parasite assemblages circulating in the studied population. We analyzed 181 fecal samples from Children at La Virgen, Cundinamarca, Colombia that were DNA-extracted and analyzed by SSU rDNA, tpi and gdh loci. We observed positivity by microscopy of 13{\%} and by PCR around 76-80{\%} depending on the molecular marker. Additionally, a lack of statistical concordance between microscopy and PCR was detected. Regarding the genetic assemblages, we detected assemblage A (3{\%}), assemblage B (90{\%}) and mixed infections assemblages A. +. B (7{\%}). Hence, the sub-assemblages were typed as AI, AII, BIII and BIV across the population. This study represents a reliable attempt to understand the molecular epidemiology of giardiasis in Colombia and the use of PCR to detect cryptic infections. The epidemiological implications are herein discussed.",
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Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombia. / Ramírez, Juan David; Heredia, Rubén Darío; Hernández, Carolina; León, Cielo M.; Moncada, Ligia Inés; Reyes, Patricia; Pinilla, Análida Elizabeth; Lopez, Myriam Consuelo.

En: Infection, Genetics and Evolution, 01.06.2015, p. 208-213.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Molecular diagnosis and genotype analysis of Giardia duodenalis in asymptomatic children from a rural area in central Colombia

AU - Ramírez, Juan David

AU - Heredia, Rubén Darío

AU - Hernández, Carolina

AU - León, Cielo M.

AU - Moncada, Ligia Inés

AU - Reyes, Patricia

AU - Pinilla, Análida Elizabeth

AU - Lopez, Myriam Consuelo

PY - 2015/6/1

Y1 - 2015/6/1

N2 - © 2015 Elsevier B.V.Giardiasis is a parasitic infection that affects around 200 million people worldwide. This parasite presents a remarkable genetic variability observed in 8 genetic clusters named as 'assemblages' (A-H). These assemblages are host restricted and could be zoonotic where A and B infect humans and animals around the globe. The knowledge of the molecular epidemiology of human giardiasis in South-America is scarce and also the usefulness of PCR to detect this pathogen in fecal samples remains controversial. The aim of this study was to conduct a cross-sectional study to compare the molecular targets employed for the molecular diagnosis of Giardia DNA and to discriminate the parasite assemblages circulating in the studied population. We analyzed 181 fecal samples from Children at La Virgen, Cundinamarca, Colombia that were DNA-extracted and analyzed by SSU rDNA, tpi and gdh loci. We observed positivity by microscopy of 13% and by PCR around 76-80% depending on the molecular marker. Additionally, a lack of statistical concordance between microscopy and PCR was detected. Regarding the genetic assemblages, we detected assemblage A (3%), assemblage B (90%) and mixed infections assemblages A. +. B (7%). Hence, the sub-assemblages were typed as AI, AII, BIII and BIV across the population. This study represents a reliable attempt to understand the molecular epidemiology of giardiasis in Colombia and the use of PCR to detect cryptic infections. The epidemiological implications are herein discussed.

AB - © 2015 Elsevier B.V.Giardiasis is a parasitic infection that affects around 200 million people worldwide. This parasite presents a remarkable genetic variability observed in 8 genetic clusters named as 'assemblages' (A-H). These assemblages are host restricted and could be zoonotic where A and B infect humans and animals around the globe. The knowledge of the molecular epidemiology of human giardiasis in South-America is scarce and also the usefulness of PCR to detect this pathogen in fecal samples remains controversial. The aim of this study was to conduct a cross-sectional study to compare the molecular targets employed for the molecular diagnosis of Giardia DNA and to discriminate the parasite assemblages circulating in the studied population. We analyzed 181 fecal samples from Children at La Virgen, Cundinamarca, Colombia that were DNA-extracted and analyzed by SSU rDNA, tpi and gdh loci. We observed positivity by microscopy of 13% and by PCR around 76-80% depending on the molecular marker. Additionally, a lack of statistical concordance between microscopy and PCR was detected. Regarding the genetic assemblages, we detected assemblage A (3%), assemblage B (90%) and mixed infections assemblages A. +. B (7%). Hence, the sub-assemblages were typed as AI, AII, BIII and BIV across the population. This study represents a reliable attempt to understand the molecular epidemiology of giardiasis in Colombia and the use of PCR to detect cryptic infections. The epidemiological implications are herein discussed.

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DO - 10.1016/j.meegid.2015.03.015

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JO - Infection, Genetics and Evolution

JF - Infection, Genetics and Evolution

SN - 1567-1348

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