Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia

Claudia Sánchez, Myriam Consuelo López, Luis Alejandro Galeano, Yvonne Qvarnstrom, Katelyn Houghton, Juan David Ramírez

Resultado de la investigación: Contribución a RevistaArtículo

4 Citas (Scopus)

Resumen

BACKGROUND: Protozoan parasites such as Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii and Entamoeba histolytica represent a great challenge to the systems producing water for human consumption because their cystic forms are persistent in the environment and resist to the disinfection methods conventionally used for their control. In this study, we investigated the presence of these protozoan pathogens in both raw and treated water samples used for the production of drinking water in Nariño Department, southwest Colombia. We collected 110 water samples (10 lof each sample) and analyzed them with real-time PCR (qPCR). qPCR-positive samples were genotyped with PCR and DNA sequencing.

RESULTS: Giardia duodenalis was detected in 35/110 (31.8%) of the samples and Cryptosporidium spp. in 9/110 (8.2%) of the samples; no sample was positive for T. gondii, E. histolytica or C. cayetanensis. Giardia duodenalis was detected in samples of both raw water (Drinking Water Treatment Plants (DWTP): 47.83%;Drinking Water Rural Plants (DWRP): 18.42%) and water collected either after conventional physicochemical treatment (26.09%) or after disinfection by chlorine (50%), whereas Cryptosporidium spp. were only detected in raw waters (DWTP: 17.39%; DWRP: 13.16%). The two pathogens were detected in both types of treatment plants supplying water to urban areas and to rural zones. Analysis of gdh and tpi markers identified assemblages AI, AII and H of G. duodenalis, while analysis of the small subunit rRNA and gp60 markers of Cryptosporidium-positive samples identified C. parvum (Subtype IIcA5G3c), C. galli, C. molnari, Cryptosporidium sp. genotype II of bats and Cryptosporidium sp. genotype VIII of birds.

CONCLUSIONS: The results obtained demonstrate the presence of protozoan parasites in the water of the study region, and the need to improve the surveillance systems for these pathogens and identify the corresponding sources of contamination.

Idioma originalEnglish (US)
Páginas (desde-hasta)563
PublicaciónParasites and Vectors
Volumen11
N.º1
DOI
EstadoPublished - oct 26 2018

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Sánchez, Claudia ; López, Myriam Consuelo ; Galeano, Luis Alejandro ; Qvarnstrom, Yvonne ; Houghton, Katelyn ; Ramírez, Juan David. / Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia. En: Parasites and Vectors. 2018 ; Vol. 11, N.º 1. pp. 563.
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title = "Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia",
abstract = "BACKGROUND: Protozoan parasites such as Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii and Entamoeba histolytica represent a great challenge to the systems producing water for human consumption because their cystic forms are persistent in the environment and resist to the disinfection methods conventionally used for their control. In this study, we investigated the presence of these protozoan pathogens in both raw and treated water samples used for the production of drinking water in Nari{\~n}o Department, southwest Colombia. We collected 110 water samples (10 lof each sample) and analyzed them with real-time PCR (qPCR). qPCR-positive samples were genotyped with PCR and DNA sequencing.RESULTS: Giardia duodenalis was detected in 35/110 (31.8{\%}) of the samples and Cryptosporidium spp. in 9/110 (8.2{\%}) of the samples; no sample was positive for T. gondii, E. histolytica or C. cayetanensis. Giardia duodenalis was detected in samples of both raw water (Drinking Water Treatment Plants (DWTP): 47.83{\%};Drinking Water Rural Plants (DWRP): 18.42{\%}) and water collected either after conventional physicochemical treatment (26.09{\%}) or after disinfection by chlorine (50{\%}), whereas Cryptosporidium spp. were only detected in raw waters (DWTP: 17.39{\%}; DWRP: 13.16{\%}). The two pathogens were detected in both types of treatment plants supplying water to urban areas and to rural zones. Analysis of gdh and tpi markers identified assemblages AI, AII and H of G. duodenalis, while analysis of the small subunit rRNA and gp60 markers of Cryptosporidium-positive samples identified C. parvum (Subtype IIcA5G3c), C. galli, C. molnari, Cryptosporidium sp. genotype II of bats and Cryptosporidium sp. genotype VIII of birds.CONCLUSIONS: The results obtained demonstrate the presence of protozoan parasites in the water of the study region, and the need to improve the surveillance systems for these pathogens and identify the corresponding sources of contamination.",
author = "Claudia S{\'a}nchez and L{\'o}pez, {Myriam Consuelo} and Galeano, {Luis Alejandro} and Yvonne Qvarnstrom and Katelyn Houghton and Ram{\'i}rez, {Juan David}",
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Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia. / Sánchez, Claudia; López, Myriam Consuelo; Galeano, Luis Alejandro; Qvarnstrom, Yvonne; Houghton, Katelyn; Ramírez, Juan David.

En: Parasites and Vectors, Vol. 11, N.º 1, 26.10.2018, p. 563.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Molecular detection and genotyping of pathogenic protozoan parasites in raw and treated water samples from southwest Colombia

AU - Sánchez, Claudia

AU - López, Myriam Consuelo

AU - Galeano, Luis Alejandro

AU - Qvarnstrom, Yvonne

AU - Houghton, Katelyn

AU - Ramírez, Juan David

PY - 2018/10/26

Y1 - 2018/10/26

N2 - BACKGROUND: Protozoan parasites such as Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii and Entamoeba histolytica represent a great challenge to the systems producing water for human consumption because their cystic forms are persistent in the environment and resist to the disinfection methods conventionally used for their control. In this study, we investigated the presence of these protozoan pathogens in both raw and treated water samples used for the production of drinking water in Nariño Department, southwest Colombia. We collected 110 water samples (10 lof each sample) and analyzed them with real-time PCR (qPCR). qPCR-positive samples were genotyped with PCR and DNA sequencing.RESULTS: Giardia duodenalis was detected in 35/110 (31.8%) of the samples and Cryptosporidium spp. in 9/110 (8.2%) of the samples; no sample was positive for T. gondii, E. histolytica or C. cayetanensis. Giardia duodenalis was detected in samples of both raw water (Drinking Water Treatment Plants (DWTP): 47.83%;Drinking Water Rural Plants (DWRP): 18.42%) and water collected either after conventional physicochemical treatment (26.09%) or after disinfection by chlorine (50%), whereas Cryptosporidium spp. were only detected in raw waters (DWTP: 17.39%; DWRP: 13.16%). The two pathogens were detected in both types of treatment plants supplying water to urban areas and to rural zones. Analysis of gdh and tpi markers identified assemblages AI, AII and H of G. duodenalis, while analysis of the small subunit rRNA and gp60 markers of Cryptosporidium-positive samples identified C. parvum (Subtype IIcA5G3c), C. galli, C. molnari, Cryptosporidium sp. genotype II of bats and Cryptosporidium sp. genotype VIII of birds.CONCLUSIONS: The results obtained demonstrate the presence of protozoan parasites in the water of the study region, and the need to improve the surveillance systems for these pathogens and identify the corresponding sources of contamination.

AB - BACKGROUND: Protozoan parasites such as Giardia duodenalis, Cryptosporidium spp., Cyclospora cayetanensis, Toxoplasma gondii and Entamoeba histolytica represent a great challenge to the systems producing water for human consumption because their cystic forms are persistent in the environment and resist to the disinfection methods conventionally used for their control. In this study, we investigated the presence of these protozoan pathogens in both raw and treated water samples used for the production of drinking water in Nariño Department, southwest Colombia. We collected 110 water samples (10 lof each sample) and analyzed them with real-time PCR (qPCR). qPCR-positive samples were genotyped with PCR and DNA sequencing.RESULTS: Giardia duodenalis was detected in 35/110 (31.8%) of the samples and Cryptosporidium spp. in 9/110 (8.2%) of the samples; no sample was positive for T. gondii, E. histolytica or C. cayetanensis. Giardia duodenalis was detected in samples of both raw water (Drinking Water Treatment Plants (DWTP): 47.83%;Drinking Water Rural Plants (DWRP): 18.42%) and water collected either after conventional physicochemical treatment (26.09%) or after disinfection by chlorine (50%), whereas Cryptosporidium spp. were only detected in raw waters (DWTP: 17.39%; DWRP: 13.16%). The two pathogens were detected in both types of treatment plants supplying water to urban areas and to rural zones. Analysis of gdh and tpi markers identified assemblages AI, AII and H of G. duodenalis, while analysis of the small subunit rRNA and gp60 markers of Cryptosporidium-positive samples identified C. parvum (Subtype IIcA5G3c), C. galli, C. molnari, Cryptosporidium sp. genotype II of bats and Cryptosporidium sp. genotype VIII of birds.CONCLUSIONS: The results obtained demonstrate the presence of protozoan parasites in the water of the study region, and the need to improve the surveillance systems for these pathogens and identify the corresponding sources of contamination.

U2 - 10.1186/s13071-018-3147-3

DO - 10.1186/s13071-018-3147-3

M3 - Article

C2 - 30367668

VL - 11

SP - 563

JO - Parasites and Vectors

JF - Parasites and Vectors

SN - 1756-3305

IS - 1

ER -