Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany

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Resumen

El Eoceno (∼ Ma) Messel Pit en Alemania es Patrimonio de la Humanidad de la UNESCO.
debido a sus fósiles excepcionalmente preservados, incluyendo vertebrados, invertebrados,
y plantas. Los vertebrados fósiles de Messel se caracterizan típicamente por su articulación
y en algunos casos la piel, el pelo, las plumas, las escamas y el contenido estomacal son
también se conservan. A pesar de la excepcional conservación macroscópica de los fósiles de Messel
vertebrados, el aspecto microestructural de estos fósiles ha sido pobremente explorado. En
estructuras particulares de tejidos blandos como el pelo o las plumas no han sido químicamente
analizadas, ni tampoco las microestructuras óseas. Reporto aquí la preservación y recuperación
de microestructuras similares a osteocitos y vasos sanguíneos del hueso de Messel Pit
incluyendo las tortugas Allaeochelys crassesculpta y Neochelys franzeni,
el cocodrilo Diplocynodon darwini, y el pangolín Eomanis krebsi. Usé un
Microscopio Electrónico de Barrido de Emisiones de Campo (FESEM) y un Phenom ProX
microscopio electrónico de barrido de sobremesa (LOT-QuantumDesign) equipado con un
fuente termoiónica CeB6 y un electrón multimodo de retrodispersión (BSE) de alta sensibilidad
para la caracterización microscópica y elemental de estas microestructuras óseas.
Las microestructuras similares a los osteocitos y a los vasos sanguíneos están constituidas por una capa delgada
(∼ nm de espesor), textura moteada externa e interna con una ligera marcación.
estrías. Las marcas circulares o lineales son comunes en la superficie externa del
y se interpretan como canales microbianos. Hierro (Fe)
es el elemento más abundante que se encuentra en los vasos sanguíneos de tipo osteocitario.
pero no en la matriz ósea ni en las microestructuras similares a las fibrilas de colágeno.
La aparición de elementos de tejidos blandos bien conservados (al menos su forma física)
establece una base prometedora para futuros estudios sobre la preservación de biomoléculas
(proteínas o ADN) en los fósiles de Messel Pit.
Idioma originalSpanish (Colombia)
Páginas (desde-hasta)e1618
Número de páginas17
PublicaciónPeerJ
Volumen4
DOI
EstadoPublished - ene 21 2016

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@article{94269058a0ac4ca7856e737687c75167,
title = "Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany",
abstract = "The Eocene (∾48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (∾50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.",
author = "Edwin Cadena",
year = "2016",
month = "1",
day = "21",
doi = "10.7717/peerj.1618",
language = "Espa{\~n}ol (Colombia)",
volume = "4",
pages = "e1618",
journal = "PeerJ",
issn = "2167-8359",
publisher = "PeerJ Inc.",

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TY - JOUR

T1 - Microscopical and elemental FESEM and Phenom ProX-SEM-EDS analysis of osteocyte- and blood vessel-like microstructures obtained from fossil vertebrates of the Eocene Messel Pit, Germany

AU - Cadena, Edwin

PY - 2016/1/21

Y1 - 2016/1/21

N2 - The Eocene (∾48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (∾50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.

AB - The Eocene (∾48 Ma) Messel Pit in Germany is a UNESCO World Heritage Site because of its exceptionally preserved fossils, including vertebrates, invertebrates, and plants. Messel fossil vertebrates are typically characterized by their articulated state, and in some cases the skin, hair, feathers, scales and stomach contents are also preserved. Despite the exceptional macroscopic preservation of Messel fossil vertebrates, the microstructural aspect of these fossils has been poorly explored. In particular, soft tissue structures such as hair or feathers have not been chemically analyzed, nor have bone microstructures. I report here the preservation and recovery of osteocyte-like and blood vessel-like microstructures from the bone of Messel Pit specimens, including the turtles Allaeochelys crassesculpta and Neochelys franzeni , the crocodile Diplocynodon darwini , and the pangolin Eomanis krebsi . I used a Field Emission Scanning Electron Microscope (FESEM) and a Phenom ProX desktop scanning electron microscope (LOT-QuantumDesign) equipped with a thermionic CeB6 source and a high sensitivity multi-mode backscatter electron (BSE) for microscopical and elemental characterization of these bone microstructures. Osteocyte-like and blood vessel-like microstructures are constituted by a thin layer (∾50 nm thickness), external and internal mottled texture with slightly marked striations. Circular to linear marks are common on the external surface of the osteocyte-like microstructures and are interpreted as microbial troughs. Iron (Fe) is the most abundant element found in the osteocyte-like and blood vessel-like microstructures, but not in the bone matrix or collagen fibril-like microstructures. The occurrence of well-preserved soft-tissue elements (at least their physical form) establishes a promising background for future studies on preservation of biomolecules (proteins or DNA) in Messel Pit fossils.

U2 - 10.7717/peerj.1618

DO - 10.7717/peerj.1618

M3 - Artículo

C2 - 26819855

VL - 4

SP - e1618

JO - PeerJ

JF - PeerJ

SN - 2167-8359

ER -