Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia

Sergio Castañeda; Marina Muñoz; Ximena Villamizar; Paula C. Hernández; Luis Reinel Vásquez; Raúl Yhossef Tito; Juan David Ramírez

doi:10.1186/s13071-020-04392-9

Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia

Título traducido de la contribución: Caracterización de la microbiota en niños colombianos en edad escolar con y sin Blastocystis

Sergio Castañeda, Marina Muñoz, Ximena Villamizar, Paula C. Hernández, Luis Reinel Vásquez, Raúl Yhossef Tito, Juan David Ramírez

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13 Citas (Scopus)

Resumen

Antecedentes: Blastocystis es un protista que vive en el tracto intestinal de una variedad de huéspedes, incluyendo a los humanos. Todavía no está claro cómo Blastocystis causa la enfermedad, lo que supone un reto permanente para los investigadores. A pesar de los controvertidos hallazgos sobre la asociación entre Blastocystis y las manifestaciones clínicas digestivas, actualmente no hay consenso sobre si este protozoo se comporta realmente como un patógeno en los seres humanos. Además, la relación entre Blastocystis y la composición de la microbiota intestinal aún no está clara. Por ello, el objetivo de este estudio fue identificar si la colonización por Blastocystis se relaciona con cambios en la diversidad y abundancia relativa de las comunidades bacterianas, en comparación con las de individuos libres de Blastocystis en un grupo de niños colombianos. Métodos: Se tomaron muestras de heces de 57 niños en edad escolar que asistían a una guardería en Popayán (suroeste de Colombia). Se extrajo el ADN completo y se examinó mediante secuenciación basada en el amplicón del ARNr 16S. Se detectó Blastocystis por PCR en tiempo real y otros parásitos intestinales por microscopía. Se evaluó si Blastocystis se asociaba a las variables del huésped y a la diversidad y abundancia de las comunidades microbianas. Resultados: La composición de la comunidad bacteriana intestinal no fue significativamente diferente entre los niños libres de Blastocystis y los colonizados por Blastocystis. A pesar de ello, observamos una mayor riqueza microbiana en los intestinos de los niños colonizados por Blastocystis, lo que podría considerarse, por tanto, un beneficio para la salud intestinal. El filo Firmicutes fue la unidad taxonómica predominante en ambos grupos analizados. En los individuos libres de Blastocystis, hubo una mayor proporción de Bacteroidetes; asimismo, en los niños colonizados por Blastocystis, hubo una mayor abundancia relativa del phylum Proteobacteria; sin embargo, no se encontraron diferencias estadísticamente significativas entre los grupos de comparación. Conclusiones: La presencia de Blastocystis mostró una disminución de Bacteroides, y un aumento en la abundancia relativa del género Faecalibacterium. También se puso de manifiesto que la presencia de Blastocystis no estaba relacionada con la disbiosis a nivel intestinal; por el contrario, su presencia no mostró diferencias estadísticas en la composición de la microbiota intestinal. No obstante, creemos que Blastocystis desempeña un papel en la ecología de la microbiota intestinal a través de su interacción con otros componentes microbianos [Figura no disponible: ver texto completo].

Título traducido de la contribución	Caracterización de la microbiota en niños colombianos en edad escolar con y sin Blastocystis
Idioma original	Inglés estadounidense
Número de artículo	521
Páginas (desde-hasta)	1-12
Número de páginas	12
Publicación	Parasites and Vectors
Volumen	13
N.º	1
DOI	https://doi.org/10.1186/s13071-020-04392-9
Estado	Publicada - oct. 16 2020

Áreas temáticas de ASJC Scopus

Parasitología
Enfermedades infecciosas

ODS de las Naciones Unidas

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10.1186/s13071-020-04392-9

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@article{d1174b896f18455d8b9fb23d018c6cb0,

title = "Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia",

abstract = "Background: Blastocystis is a protist that lives in the intestinal tract of a variety of hosts, including humans. It is still unclear how Blastocystis causes disease, which presents an ongoing challenge for researchers. Despite the controversial findings on the association between Blastocystis and clinical digestive manifestations, there is currently no consensus as to whether this protozoan actually behaves as a pathogen in humans. Furthermore, the relationship between Blastocystis and the intestinal microbiota composition is not yet clear. For that reason, the aim of this study was to identify if colonization by Blastocystis is related to changes in the diversity and relative abundance of bacterial communities, compared with those of Blastocystis-free individuals in a group of Colombian children. Methods: We took stool samples from 57 school-aged children attending a daycare institution in Popay{\'a}n (Southwest Colombia). Whole DNA was extracted and examined by 16S-rRNA amplicon-based sequencing. Blastocystis was detected by real time PCR and other intestinal parasites were detected by microscopy. We evaluated if Blastocystis was associated with host variables and the diversity and abundance of microbial communities. Results: The composition of the intestinal bacterial community was not significantly different between Blastocystis-free and Blastocystis-colonized children. Despite this, we observed a higher microbial richness in the intestines of children colonized by Blastocystis, which could, therefore, be considered a benefit to intestinal health. The phylum Firmicutes was the predominant taxonomic unit in both groups analyzed. In Blastocystis-free individuals, there was a higher proportion of Bacteroidetes; similarly, in children colonized by Blastocystis, there was a higher relative abundance of the phylum Proteobacteria; however, no statistically significant differences were found between the comparison groups. Conclusions: The presence of Blastocystis showed a decrease in Bacteroides, and an increase in the relative abundance of the genus Faecalibacterium. It was also evident that the presence of Blastocystis was unrelated to dysbiosis at the intestinal level; on the contrary, its presence did not show statistically differences in the intestinal microbiota composition. Nevertheless, we believe that Blastocystis plays a role in the ecology of the intestinal microbiota through its interaction with other microbial components.[Figure not available: see fulltext.]",

author = "Sergio Casta{\~n}eda and Marina Mu{\~n}oz and Ximena Villamizar and Hern{\'a}ndez, {Paula C.} and V{\'a}squez, {Luis Reinel} and Tito, {Ra{\'u}l Yhossef} and Ram{\'i}rez, {Juan David}",

note = "Funding Information: This study was funded by Dirreci{\'o}n de Investigaci{\'o}n e Innovaci{\'o}n of the Universidad del Rosario, Bogot{\'a}, Colombia. Acknowledgements Publisher Copyright: {\textcopyright} 2020, The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = oct,

day = "16",

doi = "10.1186/s13071-020-04392-9",

language = "English (US)",

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T1 - Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia

AU - Castañeda, Sergio

AU - Muñoz, Marina

AU - Villamizar, Ximena

AU - Hernández, Paula C.

AU - Vásquez, Luis Reinel

AU - Tito, Raúl Yhossef

AU - Ramírez, Juan David

N1 - Funding Information: This study was funded by Dirreción de Investigación e Innovación of the Universidad del Rosario, Bogotá, Colombia. Acknowledgements Publisher Copyright: © 2020, The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/10/16

Y1 - 2020/10/16

N2 - Background: Blastocystis is a protist that lives in the intestinal tract of a variety of hosts, including humans. It is still unclear how Blastocystis causes disease, which presents an ongoing challenge for researchers. Despite the controversial findings on the association between Blastocystis and clinical digestive manifestations, there is currently no consensus as to whether this protozoan actually behaves as a pathogen in humans. Furthermore, the relationship between Blastocystis and the intestinal microbiota composition is not yet clear. For that reason, the aim of this study was to identify if colonization by Blastocystis is related to changes in the diversity and relative abundance of bacterial communities, compared with those of Blastocystis-free individuals in a group of Colombian children. Methods: We took stool samples from 57 school-aged children attending a daycare institution in Popayán (Southwest Colombia). Whole DNA was extracted and examined by 16S-rRNA amplicon-based sequencing. Blastocystis was detected by real time PCR and other intestinal parasites were detected by microscopy. We evaluated if Blastocystis was associated with host variables and the diversity and abundance of microbial communities. Results: The composition of the intestinal bacterial community was not significantly different between Blastocystis-free and Blastocystis-colonized children. Despite this, we observed a higher microbial richness in the intestines of children colonized by Blastocystis, which could, therefore, be considered a benefit to intestinal health. The phylum Firmicutes was the predominant taxonomic unit in both groups analyzed. In Blastocystis-free individuals, there was a higher proportion of Bacteroidetes; similarly, in children colonized by Blastocystis, there was a higher relative abundance of the phylum Proteobacteria; however, no statistically significant differences were found between the comparison groups. Conclusions: The presence of Blastocystis showed a decrease in Bacteroides, and an increase in the relative abundance of the genus Faecalibacterium. It was also evident that the presence of Blastocystis was unrelated to dysbiosis at the intestinal level; on the contrary, its presence did not show statistically differences in the intestinal microbiota composition. Nevertheless, we believe that Blastocystis plays a role in the ecology of the intestinal microbiota through its interaction with other microbial components.[Figure not available: see fulltext.]

AB - Background: Blastocystis is a protist that lives in the intestinal tract of a variety of hosts, including humans. It is still unclear how Blastocystis causes disease, which presents an ongoing challenge for researchers. Despite the controversial findings on the association between Blastocystis and clinical digestive manifestations, there is currently no consensus as to whether this protozoan actually behaves as a pathogen in humans. Furthermore, the relationship between Blastocystis and the intestinal microbiota composition is not yet clear. For that reason, the aim of this study was to identify if colonization by Blastocystis is related to changes in the diversity and relative abundance of bacterial communities, compared with those of Blastocystis-free individuals in a group of Colombian children. Methods: We took stool samples from 57 school-aged children attending a daycare institution in Popayán (Southwest Colombia). Whole DNA was extracted and examined by 16S-rRNA amplicon-based sequencing. Blastocystis was detected by real time PCR and other intestinal parasites were detected by microscopy. We evaluated if Blastocystis was associated with host variables and the diversity and abundance of microbial communities. Results: The composition of the intestinal bacterial community was not significantly different between Blastocystis-free and Blastocystis-colonized children. Despite this, we observed a higher microbial richness in the intestines of children colonized by Blastocystis, which could, therefore, be considered a benefit to intestinal health. The phylum Firmicutes was the predominant taxonomic unit in both groups analyzed. In Blastocystis-free individuals, there was a higher proportion of Bacteroidetes; similarly, in children colonized by Blastocystis, there was a higher relative abundance of the phylum Proteobacteria; however, no statistically significant differences were found between the comparison groups. Conclusions: The presence of Blastocystis showed a decrease in Bacteroides, and an increase in the relative abundance of the genus Faecalibacterium. It was also evident that the presence of Blastocystis was unrelated to dysbiosis at the intestinal level; on the contrary, its presence did not show statistically differences in the intestinal microbiota composition. Nevertheless, we believe that Blastocystis plays a role in the ecology of the intestinal microbiota through its interaction with other microbial components.[Figure not available: see fulltext.]

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Microbiota characterization in Blastocystis-colonized and Blastocystis-free school-age children from Colombia

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