Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines

Título traducido de la contribución: Los péptidos de la proteína Mce4F Mycobacterium tuberculosis pueden inhibir la invasión de líneas celulares humanas.

Deisy Carolina Rodríguez, Marisol Ocampo, Yahson Varela, Hernando Curtidor, Manuel Alfonso Patarroyo, Manuel Elkin Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

8 Citas (Scopus)

Resumen

© FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with (125)I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide ((201)IDQAGPFLQAQIRAGGDIKSY(220)) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 ((21)LSVMAIFYLRLPATFGIGTY(40)), 38373 ((81)HMRLNSGTAIPSNVTATVRSY(100)) and 38379 ((201)IDQAGPFLQAQIRAGGDIKSY(220)) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 ((261)RPSFPALAASLANLGRVGVIY(280)) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis.
Idioma originalEnglish (US)
Número de artículo ftu020
Número de páginas12
PublicaciónPathogens and Disease
Volumen73
N.º3
DOI
EstadoPublished - abr 1 2015
Publicado de forma externa

Huella dactilar

Mycobacterium tuberculosis
Cell Line
Peptides
Proteins
Immunoelectron Microscopy
Alveolar Macrophages
Postal Service
Circular Dichroism
Microspheres
Computer Simulation
Tuberculosis
Epithelial Cells

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Rodríguez, Deisy Carolina ; Ocampo, Marisol ; Varela, Yahson ; Curtidor, Hernando ; Patarroyo, Manuel Alfonso ; Patarroyo, Manuel Elkin. / Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines. En: Pathogens and Disease. 2015 ; Vol. 73, N.º 3.
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abstract = "{\circledC} FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with (125)I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide ((201)IDQAGPFLQAQIRAGGDIKSY(220)) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 ((21)LSVMAIFYLRLPATFGIGTY(40)), 38373 ((81)HMRLNSGTAIPSNVTATVRSY(100)) and 38379 ((201)IDQAGPFLQAQIRAGGDIKSY(220)) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 ((261)RPSFPALAASLANLGRVGVIY(280)) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis.",
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Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines. / Rodríguez, Deisy Carolina; Ocampo, Marisol; Varela, Yahson; Curtidor, Hernando; Patarroyo, Manuel Alfonso; Patarroyo, Manuel Elkin.

En: Pathogens and Disease, Vol. 73, N.º 3, ftu020, 01.04.2015.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Mce4F Mycobacterium tuberculosis protein peptides can inhibit invasion of human cell lines

AU - Rodríguez, Deisy Carolina

AU - Ocampo, Marisol

AU - Varela, Yahson

AU - Curtidor, Hernando

AU - Patarroyo, Manuel Alfonso

AU - Patarroyo, Manuel Elkin

N1 - © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.

PY - 2015/4/1

Y1 - 2015/4/1

N2 - © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with (125)I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide ((201)IDQAGPFLQAQIRAGGDIKSY(220)) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 ((21)LSVMAIFYLRLPATFGIGTY(40)), 38373 ((81)HMRLNSGTAIPSNVTATVRSY(100)) and 38379 ((201)IDQAGPFLQAQIRAGGDIKSY(220)) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 ((261)RPSFPALAASLANLGRVGVIY(280)) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis.

AB - © FEMS 2014. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.This work was aimed at studying the Mycobacterium tuberculosis H37Rv Rv3494c protein, taking into account that it belongs to the mammalian cell entry family (mce) which is thought to have important functions in the disease's pathogenesis. The protein was characterized in silico; its presence on mycobacterial surface was confirmed by immunoelectron microscopy. High-activity binding peptides (HABPs) were identified by binding assays with (125)I; their ability to inhibit mycobacterial entry to two cell lines (U937 alveolar macrophages and A549 epithelial cells) was ascertained and their role in bacterial entry was confirmed by fluorescent microsphere internalization assay. This protein's predicted alpha-helix structure was confirmed by circular dichroism of its peptides. All HABPs inhibited mycobacterial entry to cells and that the 38379 peptide ((201)IDQAGPFLQAQIRAGGDIKSY(220)) had high binding ability and inhibited the mycobacterial entry to both cell lines assayed here. Rv3494c peptides 38370 ((21)LSVMAIFYLRLPATFGIGTY(40)), 38373 ((81)HMRLNSGTAIPSNVTATVRSY(100)) and 38379 ((201)IDQAGPFLQAQIRAGGDIKSY(220)) showed to be HABP and inhibited mycobacterial entry to A549 cells and peptide 38382 ((261)RPSFPALAASLANLGRVGVIY(280)) bind to U937 and inhibited the mycobacterial entry to this cell line; all of these sequences play an important role in cell line recognition and invasion, and may thus be considered in the search for prophylactic candidates against tuberculosis.

U2 - 10.1093/femspd/ftu020

DO - 10.1093/femspd/ftu020

M3 - Article

VL - 73

JO - Pathogens and Disease

JF - Pathogens and Disease

SN - 2049-632X

IS - 3

M1 - ftu020

ER -