Mammaglobin peptide as a novel biomarker for breast cancer detection

Julie M. Galvis-Jiménez, Hernando Curtidor, Manuel A. Patarroyo, Pedro Monterrey, Sandra R. Ramírez-Clavijo

Resultado de la investigación: Contribución a RevistaArtículo

6 Citas (Scopus)

Resumen

Among the different types of tests used for cancer diagnosis, molecular tests have been increrasingly incorporated because of their ability to detect either expression or functional changes in the molecules associated with the disease. Mammaglobin is a protein found in mammary tissue and can be detected in serum. This protein has been proposed as a biomarker to diagnose breast cancer, given that patients exhibit an increased amount of the protein in serum and tumor tissue, in comparison to healthy individuals. The ELISA test was used in the present study to detect mammaglobin in blood samples from 51 breast cancer patients and 51 control individuals. Antibodies against mamaglobin were generated in rabbits by using the following synthetic peptides: A (amino acids 13 to 21), B (amino acids 31 to 39), C (amino acids 56 to 64) and a D peptide, corresponding to the protein isoform without three amino acids (59, 60 and 61 amino acids) from peptide C. All peptides were immunogenic and allowed generation of antibodies that were able to discriminate patients from controls. The best results were obtained for antiserum B, achieving the best sensitivity (86.3%) and specificity (96%). © 2012 Landes Bioscience.
Idioma originalEnglish (US)
Páginas (desde-hasta)327-332
Número de páginas6
PublicaciónCancer Biology and Therapy
DOI
EstadoPublished - abr 1 2013

Huella dactilar

Biomarkers
Breast Neoplasms
Amino Acids
Peptides
Antibodies
Blood Proteins
Immune Sera
Neoplasms
Protein Isoforms
Proteins
Breast
Enzyme-Linked Immunosorbent Assay
Rabbits
Sensitivity and Specificity
Serum

Citar esto

Galvis-Jiménez, J. M., Curtidor, H., Patarroyo, M. A., Monterrey, P., & Ramírez-Clavijo, S. R. (2013). Mammaglobin peptide as a novel biomarker for breast cancer detection. Cancer Biology and Therapy, 327-332. https://doi.org/10.4161/cbt.23614
Galvis-Jiménez, Julie M. ; Curtidor, Hernando ; Patarroyo, Manuel A. ; Monterrey, Pedro ; Ramírez-Clavijo, Sandra R. / Mammaglobin peptide as a novel biomarker for breast cancer detection. En: Cancer Biology and Therapy. 2013 ; pp. 327-332.
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abstract = "Among the different types of tests used for cancer diagnosis, molecular tests have been increrasingly incorporated because of their ability to detect either expression or functional changes in the molecules associated with the disease. Mammaglobin is a protein found in mammary tissue and can be detected in serum. This protein has been proposed as a biomarker to diagnose breast cancer, given that patients exhibit an increased amount of the protein in serum and tumor tissue, in comparison to healthy individuals. The ELISA test was used in the present study to detect mammaglobin in blood samples from 51 breast cancer patients and 51 control individuals. Antibodies against mamaglobin were generated in rabbits by using the following synthetic peptides: A (amino acids 13 to 21), B (amino acids 31 to 39), C (amino acids 56 to 64) and a D peptide, corresponding to the protein isoform without three amino acids (59, 60 and 61 amino acids) from peptide C. All peptides were immunogenic and allowed generation of antibodies that were able to discriminate patients from controls. The best results were obtained for antiserum B, achieving the best sensitivity (86.3{\%}) and specificity (96{\%}). {\circledC} 2012 Landes Bioscience.",
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Galvis-Jiménez, JM, Curtidor, H, Patarroyo, MA, Monterrey, P & Ramírez-Clavijo, SR 2013, 'Mammaglobin peptide as a novel biomarker for breast cancer detection', Cancer Biology and Therapy, pp. 327-332. https://doi.org/10.4161/cbt.23614

Mammaglobin peptide as a novel biomarker for breast cancer detection. / Galvis-Jiménez, Julie M.; Curtidor, Hernando; Patarroyo, Manuel A.; Monterrey, Pedro; Ramírez-Clavijo, Sandra R.

En: Cancer Biology and Therapy, 01.04.2013, p. 327-332.

Resultado de la investigación: Contribución a RevistaArtículo

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AU - Galvis-Jiménez, Julie M.

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AB - Among the different types of tests used for cancer diagnosis, molecular tests have been increrasingly incorporated because of their ability to detect either expression or functional changes in the molecules associated with the disease. Mammaglobin is a protein found in mammary tissue and can be detected in serum. This protein has been proposed as a biomarker to diagnose breast cancer, given that patients exhibit an increased amount of the protein in serum and tumor tissue, in comparison to healthy individuals. The ELISA test was used in the present study to detect mammaglobin in blood samples from 51 breast cancer patients and 51 control individuals. Antibodies against mamaglobin were generated in rabbits by using the following synthetic peptides: A (amino acids 13 to 21), B (amino acids 31 to 39), C (amino acids 56 to 64) and a D peptide, corresponding to the protein isoform without three amino acids (59, 60 and 61 amino acids) from peptide C. All peptides were immunogenic and allowed generation of antibodies that were able to discriminate patients from controls. The best results were obtained for antiserum B, achieving the best sensitivity (86.3%) and specificity (96%). © 2012 Landes Bioscience.

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