Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice

K. Darragh, S. Vanjari, F. Mann, M.F. Gonzalez-Rojas, C.R. Morrison, C. Salazar, C. Pardo-Diaz, R.M. Merrill, W.O. McMillan, S. Schulz, C.D. Jiggins

Resultado de la investigación: Contribución a RevistaArtículo

9 Citas (Scopus)

Resumen

Sex-specific pheromones are known to play an important role in butterfly courtship, and may influence both individual reproductive success and reproductive isolation between species. Extensive ecological, behavioural and genetic studies of Heliconius butterflies have made a substantial contribution to our understanding of speciation. Male pheromones, although long suspected to play an important role, have received relatively little attention in this genus. Here, we combine morphological, chemical and behavioural analyses of male pheromones in the Neotropical butterfly Heliconius melpomene. First, we identify putative androconia that are specialized brush-like scales that lie within the shiny grey region of the male hindwing. We then describe putative male sex pheromone compounds, which are largely confined to the androconial region of the hindwing of mature males, but are absent in immature males and females. Finally, behavioural choice experiments reveal that females of H. melpomene, H. erato and H. timareta strongly discriminate against conspecific males which have their androconial region experimentally blocked. As well as demonstrating the importance of chemical signalling for female mate choice in Heliconius butterflies, the results describe structures involved in release of the pheromone and a list of potential male sex pheromone compounds. © 2017 Darragh et al.
Idioma originalEnglish
PublicaciónPeerJ
Volumen2017
N.º11
DOI
EstadoPublished - 2017

Huella dactilar

Heliconius
Sex Attractants
Butterflies
Pheromones
sex pheromones
butterflies
pheromones
Brushes
Reproductive Isolation
Behavioral Genetics
Courtship
reproductive isolation
mating behavior
courtship
Experiments
immatures
gender

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Darragh, K., Vanjari, S., Mann, F., Gonzalez-Rojas, M. F., Morrison, C. R., Salazar, C., ... Jiggins, C. D. (2017). Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice. PeerJ, 2017(11). https://doi.org/10.7717/peerj.3953
Darragh, K. ; Vanjari, S. ; Mann, F. ; Gonzalez-Rojas, M.F. ; Morrison, C.R. ; Salazar, C. ; Pardo-Diaz, C. ; Merrill, R.M. ; McMillan, W.O. ; Schulz, S. ; Jiggins, C.D. / Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice. En: PeerJ. 2017 ; Vol. 2017, N.º 11.
@article{ce4ad4c0c38c4fed9a96120bb80fea6a,
title = "Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice",
abstract = "Sex-specific pheromones are known to play an important role in butterfly courtship, and may influence both individual reproductive success and reproductive isolation between species. Extensive ecological, behavioural and genetic studies of Heliconius butterflies have made a substantial contribution to our understanding of speciation. Male pheromones, although long suspected to play an important role, have received relatively little attention in this genus. Here, we combine morphological, chemical and behavioural analyses of male pheromones in the Neotropical butterfly Heliconius melpomene. First, we identify putative androconia that are specialized brush-like scales that lie within the shiny grey region of the male hindwing. We then describe putative male sex pheromone compounds, which are largely confined to the androconial region of the hindwing of mature males, but are absent in immature males and females. Finally, behavioural choice experiments reveal that females of H. melpomene, H. erato and H. timareta strongly discriminate against conspecific males which have their androconial region experimentally blocked. As well as demonstrating the importance of chemical signalling for female mate choice in Heliconius butterflies, the results describe structures involved in release of the pheromone and a list of potential male sex pheromone compounds. {\circledC} 2017 Darragh et al.",
author = "K. Darragh and S. Vanjari and F. Mann and M.F. Gonzalez-Rojas and C.R. Morrison and C. Salazar and C. Pardo-Diaz and R.M. Merrill and W.O. McMillan and S. Schulz and C.D. Jiggins",
note = "Cited By :1 Export Date: 27 February 2018 Correspondence Address: Jiggins, C.D.; Department of Zoology, University of Cambridge, CambridgeUnited Kingdom; email: c.jiggins@zoo.cam.ac.uk Funding details: QDN-DG001, UR, Universidad del Rosario Funding details: FP44842-5-2017, COLCIENCIAS, Departamento Administrativo de Ciencia, Tecnolog{\'i}a e Innovaci{\'o}n Funding details: STRI, Smithsonian Tropical Research Institute Funding details: DEB 1257689, STRI, Smithsonian Tropical Research Institute Funding details: 339873, ERC, H2020 European Research Council Funding details: NERC, Natural Environment Research Council Funding details: DEB 1257689, NSF, National Science Foundation Funding text: We acknowledge the support of Caroline Nieberding and Christer L{\"o}fstedt who provided early encouragement to pursue this project, and Kelsey Byers who gave useful advice on data analysis and manuscript preparation. We also thank Andrew Mongue and an anonymous reviewer who provided helpful comments on the manuscript. We thank our team at the insectaries in Panama, including Oscar Paneso, Sylvia Fernanda Garza Reyes and Rachel Crisp. We also thank Oscar Penagos for his technical support breeding butterflies at insectaries in La Vega, Colombia. Funding text: KD is funded by a Natural Environment Research Council Doctoral Training Partnership. SJ was funded by a Manmohan Singh studentship from St John’s College. RMM was funded by a Junior Research Fellowship at King’s College, Cambridge. CDJ and RMM are supported by a European Research Council grant number 339873 Speciation Genetics. MFG, CS and CPD were funded by the Universidad del Rosario FIUR grant QDN-DG001 and COLCIENCIAS (Grant FP44842-5-2017). WOM was supported by the Smithsonian Tropical Research Institute and NSF grant DEB 1257689. CRM was supported by a STRI Predoctoral Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding text: The following grant information was disclosed by the authors: Natural Environment Research Council. Doctoral Training Partnership. Manmohan Singh studentship. Junior Research Fellowship. European Research Council: 339873. Universidad del Rosario FIUR: QDN-DG001. COLCIENCIAS: FP44842-5-2017. Smithsonian Tropical Research Institute and NSF: DEB 1257689. References: Ando, T., Inomata, S., Yamamoto, M., (2004) Lepidopteran sex pheromones, , In: Schulz S, ed. The chemistry of pheromones and other semiochemicals I. Topics in current chemistry. 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Darragh, K, Vanjari, S, Mann, F, Gonzalez-Rojas, MF, Morrison, CR, Salazar, C, Pardo-Diaz, C, Merrill, RM, McMillan, WO, Schulz, S & Jiggins, CD 2017, 'Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice', PeerJ, vol. 2017, n.º 11. https://doi.org/10.7717/peerj.3953

Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice. / Darragh, K.; Vanjari, S.; Mann, F.; Gonzalez-Rojas, M.F.; Morrison, C.R.; Salazar, C.; Pardo-Diaz, C.; Merrill, R.M.; McMillan, W.O.; Schulz, S.; Jiggins, C.D.

En: PeerJ, Vol. 2017, N.º 11, 2017.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Male sex pheromone components in Heliconius butterflies released by the androconia affect female choice

AU - Darragh, K.

AU - Vanjari, S.

AU - Mann, F.

AU - Gonzalez-Rojas, M.F.

AU - Morrison, C.R.

AU - Salazar, C.

AU - Pardo-Diaz, C.

AU - Merrill, R.M.

AU - McMillan, W.O.

AU - Schulz, S.

AU - Jiggins, C.D.

N1 - Cited By :1 Export Date: 27 February 2018 Correspondence Address: Jiggins, C.D.; Department of Zoology, University of Cambridge, CambridgeUnited Kingdom; email: c.jiggins@zoo.cam.ac.uk Funding details: QDN-DG001, UR, Universidad del Rosario Funding details: FP44842-5-2017, COLCIENCIAS, Departamento Administrativo de Ciencia, Tecnología e Innovación Funding details: STRI, Smithsonian Tropical Research Institute Funding details: DEB 1257689, STRI, Smithsonian Tropical Research Institute Funding details: 339873, ERC, H2020 European Research Council Funding details: NERC, Natural Environment Research Council Funding details: DEB 1257689, NSF, National Science Foundation Funding text: We acknowledge the support of Caroline Nieberding and Christer Löfstedt who provided early encouragement to pursue this project, and Kelsey Byers who gave useful advice on data analysis and manuscript preparation. We also thank Andrew Mongue and an anonymous reviewer who provided helpful comments on the manuscript. We thank our team at the insectaries in Panama, including Oscar Paneso, Sylvia Fernanda Garza Reyes and Rachel Crisp. We also thank Oscar Penagos for his technical support breeding butterflies at insectaries in La Vega, Colombia. Funding text: KD is funded by a Natural Environment Research Council Doctoral Training Partnership. SJ was funded by a Manmohan Singh studentship from St John’s College. RMM was funded by a Junior Research Fellowship at King’s College, Cambridge. CDJ and RMM are supported by a European Research Council grant number 339873 Speciation Genetics. MFG, CS and CPD were funded by the Universidad del Rosario FIUR grant QDN-DG001 and COLCIENCIAS (Grant FP44842-5-2017). WOM was supported by the Smithsonian Tropical Research Institute and NSF grant DEB 1257689. CRM was supported by a STRI Predoctoral Fellowship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Funding text: The following grant information was disclosed by the authors: Natural Environment Research Council. Doctoral Training Partnership. Manmohan Singh studentship. Junior Research Fellowship. European Research Council: 339873. Universidad del Rosario FIUR: QDN-DG001. COLCIENCIAS: FP44842-5-2017. Smithsonian Tropical Research Institute and NSF: DEB 1257689. References: Ando, T., Inomata, S., Yamamoto, M., (2004) Lepidopteran sex pheromones, , In: Schulz S, ed. The chemistry of pheromones and other semiochemicals I. Topics in current chemistry. 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PY - 2017

Y1 - 2017

N2 - Sex-specific pheromones are known to play an important role in butterfly courtship, and may influence both individual reproductive success and reproductive isolation between species. Extensive ecological, behavioural and genetic studies of Heliconius butterflies have made a substantial contribution to our understanding of speciation. Male pheromones, although long suspected to play an important role, have received relatively little attention in this genus. Here, we combine morphological, chemical and behavioural analyses of male pheromones in the Neotropical butterfly Heliconius melpomene. First, we identify putative androconia that are specialized brush-like scales that lie within the shiny grey region of the male hindwing. We then describe putative male sex pheromone compounds, which are largely confined to the androconial region of the hindwing of mature males, but are absent in immature males and females. Finally, behavioural choice experiments reveal that females of H. melpomene, H. erato and H. timareta strongly discriminate against conspecific males which have their androconial region experimentally blocked. As well as demonstrating the importance of chemical signalling for female mate choice in Heliconius butterflies, the results describe structures involved in release of the pheromone and a list of potential male sex pheromone compounds. © 2017 Darragh et al.

AB - Sex-specific pheromones are known to play an important role in butterfly courtship, and may influence both individual reproductive success and reproductive isolation between species. Extensive ecological, behavioural and genetic studies of Heliconius butterflies have made a substantial contribution to our understanding of speciation. Male pheromones, although long suspected to play an important role, have received relatively little attention in this genus. Here, we combine morphological, chemical and behavioural analyses of male pheromones in the Neotropical butterfly Heliconius melpomene. First, we identify putative androconia that are specialized brush-like scales that lie within the shiny grey region of the male hindwing. We then describe putative male sex pheromone compounds, which are largely confined to the androconial region of the hindwing of mature males, but are absent in immature males and females. Finally, behavioural choice experiments reveal that females of H. melpomene, H. erato and H. timareta strongly discriminate against conspecific males which have their androconial region experimentally blocked. As well as demonstrating the importance of chemical signalling for female mate choice in Heliconius butterflies, the results describe structures involved in release of the pheromone and a list of potential male sex pheromone compounds. © 2017 Darragh et al.

U2 - 10.7717/peerj.3953

DO - 10.7717/peerj.3953

M3 - Artículo

VL - 2017

JO - PeerJ

JF - PeerJ

SN - 2167-8359

IS - 11

ER -