Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels

Título traducido de la contribución: La toxoplasmosis ocular severa de Sudamérica está asociada con disminución de Ifn-γ / Il-17a y aumento de los niveles intraoculares Il-6 / Il-13

Alejandra de-la-Torre, Arnaud Sauer, Alexander W. Pfaff, Tristan Bourcier, Julie Brunet, Claude Speeg-Schatz, Laurent Ballonzoli, Odile Villard, Daniel Ajzenberg, Natarajan Sundar, Michael E. Grigg, Jorge E. Gomez-Marin, Ermanno Candolfi

Resultado de la investigación: Contribución a RevistaArtículo

44 Citas (Scopus)

Resumen

En un estudio transversal, se evaluaron 19 casos franceses y 23 colombianos de toxoplasmosis ocular activa confirmada (OT). El objetivo fue comparar las respuestas clínicas, parasitológicas e inmunológicas y relacionarlas con las cepas infectantes. Se realizó un examen ocular completo en cada paciente. La cepa infectante se caracterizó por genotipado cuando el ADN de Toxoplasma intraocular era detectable, así como por serotipado específico de péptido para cada paciente. Para caracterizar la respuesta inmune, evaluamos los patrones de reconocimiento de proteínas de Toxoplasma por anticuerpos intraoculares y el perfil intraocular de citoquinas, quimiocinas y factores de crecimiento. Se encontraron diferencias significativas para el tamaño de las lesiones activas, la afectación macular unilateral, la discapacidad visual unilateral, la inflamación vítrea, las sinequias y la vasculitis, y se observaron valores más altos en todos los pacientes colombianos. El genotipado de la secuencia de PCR-ADN de Multilocus solo fue exitoso en tres pacientes colombianos que revelaron un tipo I y dos cepas atípicas. Los pacientes con OT colombianos poseían serotipos atípicos heterogéneos mientras que los franceses eran uniformemente reactivos a los péptidos de la cepa tipo II. Los patrones de proteína reconocidos por los anticuerpos intraoculares y los patrones de citoquinas fueron sorprendentemente diferentes entre las dos poblaciones. La expresión intraocular de IFN-γ e IL-17 fue menor, mientras que se detectaron niveles más altos de IL-13 e IL-6 en el humor acuoso de pacientes colombianos. Nuestros resultados son consistentes con la hipótesis de que las cepas sudamericanas pueden causar OT más severa debido a una inhibición del efecto protector del IFN-γ.
Idioma originalEnglish (US)
PublicaciónPLoS Neglected Tropical Diseases
DOI
EstadoPublished - nov 1 2013

Huella dactilar

Ocular Toxoplasmosis
Toxoplasma
Cytokines
Serotyping
Peptides
Interleukin-13
Interleukin-17
Antibodies
Aqueous Humor
Vision Disorders
Vasculitis
Chemokines
Interleukin-6
Intercellular Signaling Peptides and Proteins
Proteins
Cross-Sectional Studies
Inflammation
Polymerase Chain Reaction
DNA
Population

Citar esto

de-la-Torre, Alejandra ; Sauer, Arnaud ; Pfaff, Alexander W. ; Bourcier, Tristan ; Brunet, Julie ; Speeg-Schatz, Claude ; Ballonzoli, Laurent ; Villard, Odile ; Ajzenberg, Daniel ; Sundar, Natarajan ; Grigg, Michael E. ; Gomez-Marin, Jorge E. ; Candolfi, Ermanno. / Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels. En: PLoS Neglected Tropical Diseases. 2013.
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title = "Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels",
abstract = "In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.",
author = "Alejandra de-la-Torre and Arnaud Sauer and Pfaff, {Alexander W.} and Tristan Bourcier and Julie Brunet and Claude Speeg-Schatz and Laurent Ballonzoli and Odile Villard and Daniel Ajzenberg and Natarajan Sundar and Grigg, {Michael E.} and Gomez-Marin, {Jorge E.} and Ermanno Candolfi",
year = "2013",
month = "11",
day = "1",
doi = "10.1371/journal.pntd.0002541",
language = "English (US)",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2727",
publisher = "Public Library of Science",

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de-la-Torre, A, Sauer, A, Pfaff, AW, Bourcier, T, Brunet, J, Speeg-Schatz, C, Ballonzoli, L, Villard, O, Ajzenberg, D, Sundar, N, Grigg, ME, Gomez-Marin, JE & Candolfi, E 2013, 'Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels', PLoS Neglected Tropical Diseases. https://doi.org/10.1371/journal.pntd.0002541

Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels. / de-la-Torre, Alejandra; Sauer, Arnaud; Pfaff, Alexander W.; Bourcier, Tristan; Brunet, Julie; Speeg-Schatz, Claude; Ballonzoli, Laurent; Villard, Odile; Ajzenberg, Daniel; Sundar, Natarajan; Grigg, Michael E.; Gomez-Marin, Jorge E.; Candolfi, Ermanno.

En: PLoS Neglected Tropical Diseases, 01.11.2013.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Severe South American Ocular Toxoplasmosis Is Associated with Decreased Ifn-γ/Il-17a and Increased Il-6/Il-13 Intraocular Levels

AU - de-la-Torre, Alejandra

AU - Sauer, Arnaud

AU - Pfaff, Alexander W.

AU - Bourcier, Tristan

AU - Brunet, Julie

AU - Speeg-Schatz, Claude

AU - Ballonzoli, Laurent

AU - Villard, Odile

AU - Ajzenberg, Daniel

AU - Sundar, Natarajan

AU - Grigg, Michael E.

AU - Gomez-Marin, Jorge E.

AU - Candolfi, Ermanno

PY - 2013/11/1

Y1 - 2013/11/1

N2 - In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.

AB - In a cross sectional study, 19 French and 23 Colombian cases of confirmed active ocular toxoplasmosis (OT) were evaluated. The objective was to compare clinical, parasitological and immunological responses and relate them to the infecting strains. A complete ocular examination was performed in each patient. The infecting strain was characterized by genotyping when intraocular Toxoplasma DNA was detectable, as well as by peptide-specific serotyping for each patient. To characterize the immune response, we assessed Toxoplasma protein recognition patterns by intraocular antibodies and the intraocular profile of cytokines, chemokines and growth factors. Significant differences were found for size of active lesions, unilateral macular involvement, unilateral visual impairment, vitreous inflammation, synechiae, and vasculitis, with higher values observed throughout for Colombian patients. Multilocus PCR-DNA sequence genotyping was only successful in three Colombian patients revealing one type I and two atypical strains. The Colombian OT patients possessed heterogeneous atypical serotypes whereas the French were uniformly reactive to type II strain peptides. The protein patterns recognized by intraocular antibodies and the cytokine patterns were strikingly different between the two populations. Intraocular IFN-γ and IL-17 expression was lower, while higher levels of IL-13 and IL-6 were detected in aqueous humor of Colombian patients. Our results are consistent with the hypothesis that South American strains may cause more severe OT due to an inhibition of the protective effect of IFN-γ.

U2 - 10.1371/journal.pntd.0002541

DO - 10.1371/journal.pntd.0002541

M3 - Article

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2727

ER -