Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon

Fábio Brito-Santos; Luciana Trilles; Carolina Firacative; Bodo Wanke; Filipe Anibal Carvalho-Costa; Marília Martins Nishikawa; Jonas Pereira Campos; Angela Cristina Veríssimo Junqueira; Amanda Coutinho de Souza; Márcia Dos Santos Lazéra; Wieland Meyer

doi:10.3390/microorganisms8050682

Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon

Título traducido de la contribución: El polvo en espacios cerrados como fuente de cepas virulentas de los agentes de la criptococosis en la Microrregión del Río Negro de la Amazonia brasileña

Fábio Brito-Santos
, Luciana Trilles
, Carolina Firacative
, Bodo Wanke
, Filipe Anibal Carvalho-Costa
, Marília Martins Nishikawa
, Jonas Pereira Campos
, Angela Cristina Veríssimo Junqueira
, Amanda Coutinho de Souza
, Márcia Dos Santos Lazéra
, Wieland Meyer

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

12 Citas (Scopus)

Resumen

La criptococosis, una micosis potencialmente mortal en humanos, se adquiere a través de la exposición a fuentes ambientales exógenas. Este estudio tuvo como objetivo investigar la frecuencia, diversidad genética y virulencia de cepas criptocócicas aisladas del polvo interior en la microrregión de Río Negro de la Amazonia brasileña. Un total de 8,9% de las casas estudiadas fueron positivas, recuperando nueve aislamientos de Cryptococcus neoformans VNI y 16 de C. gattii VGII, revelando un patrón endémico en microambientes domésticos. El esquema de consenso de la Sociedad Internacional de Micología Humana y Animal (ISHAM) de tipificación de secuencias multilocus (MLST) para C. neoformans/C. Los complejos de especies de C. gattii identificaron dos tipos de secuencia (ST), ST93 y ST5, entre los aislamientos de C. neoformans y seis ST entre los aislamientos de C. gattii, incluidos los ST7 (VGIIa) y ST20 (VGIIb) del brote de la isla de Vancouver, el ST5 australiano y los ST264, ST268 y ST445, que son exclusivos de la región estudiada. Los estudios de virulencia en el modelo de Galleriamellonella mostraron que cinco cepas de C. gattii y una cepa de C. neoformans mostraron un potencial patógeno similar a la cepa altamente virulenta CDR265 (VGIIa) del brote de la isla de Vancouver. Los hallazgos de este estudio indican que los humanos pueden estar expuestos a los agentes de la criptococosis a través del polvo doméstico, lo que forma la base para futuros estudios para analizar el impacto de la exposición temprana y continua al polvo en interiores en el desarrollo de infecciones subclínicas o clínicas.

Título traducido de la contribución	El polvo en espacios cerrados como fuente de cepas virulentas de los agentes de la criptococosis en la Microrregión del Río Negro de la Amazonia brasileña
Idioma original	Inglés estadounidense
Número de artículo	682
Publicación	Microorganisms
Volumen	8
N.º	5
DOI	https://doi.org/10.3390/microorganisms8050682
Estado	Publicada - may. 7 2020

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ODS 3: Salud y bienestar

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Microbiología (médica)

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Brito-Santos, F., Trilles, L., Firacative, C., Wanke, B., Carvalho-Costa, F. A., Nishikawa, M. M., Campos, J. P., Junqueira, A. C. V., Souza, A. C. D., Lazéra, M. D. S., & Meyer, W. (2020). Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon. Microorganisms, 8(5), Artículo 682. https://doi.org/10.3390/microorganisms8050682

@article{1ddfc13ee2ee4a19a738406be33895eb,

title = "Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon",

abstract = "Cryptococcosis, a potentially fatal mycosis in humans, is acquired via exposure to exogenous environmental sources. This study aimed to investigate the frequency, genetic diversity, and virulence of cryptococcal strains isolated from indoor dust in the Rio Negro micro-region of the Brazilian Amazon. A total of 8.9\% of the studied houses were positive, recovering nine Cryptococcus neoformans VNI and 16 C. gattii VGII isolates, revealing an endemic pattern in domestic microenvironments. The International Society for Human and Animal Mycology (ISHAM) consensus multilocus sequence typing (MLST) scheme for the C.\ neoformans/C. gattii species complexes identified two sequence types (STs), ST93 and ST5, amongst C. neoformans isolates and six STs amongst C. gattii isolates, including the Vancouver Island Outbreak ST7 (VGIIa) and ST20 (VGIIb), the Australian ST5, and ST264, ST268 and ST445, being unique to the studied region. Virulence studies in the Galleriamellonella model showed that five C.gattii strains and one C. neoformans strain showed a similar pathogenic potential to the highly virulent Vancouver Island outbreak strain CDR265 (VGIIa). The findings of this study indicate that humans can be exposed to the agents of cryptococcosis via house dust, forming the basis for future studies to analyze the impact of early and continuous exposure to indoor dust on the development of subclinical or clinical infections.",

author = "F{\'a}bio Brito-Santos and Luciana Trilles and Carolina Firacative and Bodo Wanke and Carvalho-Costa, \{Filipe Anibal\} and Nishikawa, \{Mar{\'i}lia Martins\} and Campos, \{Jonas Pereira\} and Junqueira, \{Angela Cristina Ver{\'i}ssimo\} and Souza, \{Amanda Coutinho de\} and Laz{\'e}ra, \{M{\'a}rcia Dos Santos\} and Wieland Meyer",

year = "2020",

month = may,

day = "7",

doi = "10.3390/microorganisms8050682",

language = "English (US)",

volume = "8",

journal = "Microorganisms",

issn = "2076-2607",

publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",

number = "5",

}

Brito-Santos, F, Trilles, L, Firacative, C, Wanke, B, Carvalho-Costa, FA, Nishikawa, MM, Campos, JP, Junqueira, ACV, Souza, ACD, Lazéra, MDS & Meyer, W 2020, 'Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon', Microorganisms, vol. 8, n.º 5, 682. https://doi.org/10.3390/microorganisms8050682

TY - JOUR

T1 - Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon

AU - Brito-Santos, Fábio

AU - Trilles, Luciana

AU - Firacative, Carolina

AU - Wanke, Bodo

AU - Carvalho-Costa, Filipe Anibal

AU - Nishikawa, Marília Martins

AU - Campos, Jonas Pereira

AU - Junqueira, Angela Cristina Veríssimo

AU - Souza, Amanda Coutinho de

AU - Lazéra, Márcia Dos Santos

AU - Meyer, Wieland

PY - 2020/5/7

Y1 - 2020/5/7

N2 - Cryptococcosis, a potentially fatal mycosis in humans, is acquired via exposure to exogenous environmental sources. This study aimed to investigate the frequency, genetic diversity, and virulence of cryptococcal strains isolated from indoor dust in the Rio Negro micro-region of the Brazilian Amazon. A total of 8.9% of the studied houses were positive, recovering nine Cryptococcus neoformans VNI and 16 C. gattii VGII isolates, revealing an endemic pattern in domestic microenvironments. The International Society for Human and Animal Mycology (ISHAM) consensus multilocus sequence typing (MLST) scheme for the C. neoformans/C. gattii species complexes identified two sequence types (STs), ST93 and ST5, amongst C. neoformans isolates and six STs amongst C. gattii isolates, including the Vancouver Island Outbreak ST7 (VGIIa) and ST20 (VGIIb), the Australian ST5, and ST264, ST268 and ST445, being unique to the studied region. Virulence studies in the Galleriamellonella model showed that five C.gattii strains and one C. neoformans strain showed a similar pathogenic potential to the highly virulent Vancouver Island outbreak strain CDR265 (VGIIa). The findings of this study indicate that humans can be exposed to the agents of cryptococcosis via house dust, forming the basis for future studies to analyze the impact of early and continuous exposure to indoor dust on the development of subclinical or clinical infections.

AB - Cryptococcosis, a potentially fatal mycosis in humans, is acquired via exposure to exogenous environmental sources. This study aimed to investigate the frequency, genetic diversity, and virulence of cryptococcal strains isolated from indoor dust in the Rio Negro micro-region of the Brazilian Amazon. A total of 8.9% of the studied houses were positive, recovering nine Cryptococcus neoformans VNI and 16 C. gattii VGII isolates, revealing an endemic pattern in domestic microenvironments. The International Society for Human and Animal Mycology (ISHAM) consensus multilocus sequence typing (MLST) scheme for the C. neoformans/C. gattii species complexes identified two sequence types (STs), ST93 and ST5, amongst C. neoformans isolates and six STs amongst C. gattii isolates, including the Vancouver Island Outbreak ST7 (VGIIa) and ST20 (VGIIb), the Australian ST5, and ST264, ST268 and ST445, being unique to the studied region. Virulence studies in the Galleriamellonella model showed that five C.gattii strains and one C. neoformans strain showed a similar pathogenic potential to the highly virulent Vancouver Island outbreak strain CDR265 (VGIIa). The findings of this study indicate that humans can be exposed to the agents of cryptococcosis via house dust, forming the basis for future studies to analyze the impact of early and continuous exposure to indoor dust on the development of subclinical or clinical infections.

U2 - 10.3390/microorganisms8050682

DO - 10.3390/microorganisms8050682

M3 - Research Article

C2 - 32392852

SN - 2076-2607

VL - 8

JO - Microorganisms

JF - Microorganisms

IS - 5

M1 - 682

ER -

Indoor dust as a source of virulent strains of the agents of cryptococcosis in the Rio Negro Micro-Region of the Brazilian Amazon

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