In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes

Jose Rojas-Caraballo, Julio López-Abán, Luis Pérez Del Villar, Carolina Vizcaíno, Belén Vicente, Pedro Fernández-Soto, Esther Del Olmo, Manuel Alfonso Patarroyo, Antonio Muro

    Resultado de la investigación: Contribución a RevistaArtículo

    14 Citas (Scopus)

    Resumen

    Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p
    Idioma originalEnglish (US)
    PublicaciónPLoS One
    DOI
    EstadoPublished - ago 14 2014

    Huella dactilar

    Peptide T
    B-Lymphocyte Epitopes
    Fasciola hepatica
    Aptitude
    T-Lymphocyte Epitopes
    synthetic peptides
    in vivo studies
    epitopes
    B-lymphocytes
    T-lymphocytes
    immune response
    peptides
    Peptides
    triclabendazole
    fascioliasis
    Immunoglobulin G
    metacercariae
    mice
    World Health Organization
    Metacercariae

    Citar esto

    Rojas-Caraballo, Jose ; López-Abán, Julio ; Pérez Del Villar, Luis ; Vizcaíno, Carolina ; Vicente, Belén ; Fernández-Soto, Pedro ; Del Olmo, Esther ; Patarroyo, Manuel Alfonso ; Muro, Antonio. / In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes. En: PLoS One. 2014.
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    title = "In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes",
    abstract = "Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p",
    author = "Jose Rojas-Caraballo and Julio L{\'o}pez-Ab{\'a}n and {P{\'e}rez Del Villar}, Luis and Carolina Vizca{\'i}no and Bel{\'e}n Vicente and Pedro Fern{\'a}ndez-Soto and {Del Olmo}, Esther and Patarroyo, {Manuel Alfonso} and Antonio Muro",
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    In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes. / Rojas-Caraballo, Jose; López-Abán, Julio; Pérez Del Villar, Luis; Vizcaíno, Carolina; Vicente, Belén; Fernández-Soto, Pedro; Del Olmo, Esther; Patarroyo, Manuel Alfonso; Muro, Antonio.

    En: PLoS One, 14.08.2014.

    Resultado de la investigación: Contribución a RevistaArtículo

    TY - JOUR

    T1 - In vitro and in vivo studies for assessing the immune response and protection-inducing ability conferred by Fasciola hepatica-derived synthetic peptides containing B- and T-cell epitopes

    AU - Rojas-Caraballo, Jose

    AU - López-Abán, Julio

    AU - Pérez Del Villar, Luis

    AU - Vizcaíno, Carolina

    AU - Vicente, Belén

    AU - Fernández-Soto, Pedro

    AU - Del Olmo, Esther

    AU - Patarroyo, Manuel Alfonso

    AU - Muro, Antonio

    PY - 2014/8/14

    Y1 - 2014/8/14

    N2 - Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p

    AB - Fasciolosis is considered the most widespread trematode disease affecting grazing animals around the world; it is currently recognised by the World Health Organisation as an emergent human pathogen. Triclabendazole is still the most effective drug against this disease; however, resistant strains have appeared and developing an effective vaccine against this disease has increasingly become a priority. Several bioinformatics tools were here used for predicting B- and T-cell epitopes according to the available data for Fasciola hepatica protein amino acid sequences. BALB/c mice were immunised with the synthetic peptides by using the ADAD vaccination system and several immune response parameters were measured (antibody titres, cytokine levels, T-cell populations) to evaluate their ability to elicit an immune response. Based on the immunogenicity results so obtained, seven peptides were selected to assess their protection-inducing ability against experimental infection with F. hepatica metacercariae. Twenty-four B- or T-epitope-containing peptides were predicted and chemically synthesised. Immunisation of mice with peptides so-called B1, B2, B5, B6, T14, T15 and T16 induced high levels of total IgG, IgG1 and IgG2a (p

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    M3 - Article

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