In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51

Adriana Patricia Corredor, Janneth González, Luis Alfredo Baquero, Hernando Curtidor, Nury Nathalia Olaya-Galán, Manuel Alfonso Patarroyo, María Fernanda Gutiérrez

Resultado de la investigación: Contribución a RevistaArtículo

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Resumen

The envelope glycoprotein 51 (gp51) is essential for bovine leukaemia virus (BLV) entry to bovine B-lymphocytes. Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools. The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. The results showed that the participation of 6 key amino acids (aa) on gp51 (Asn170, Trp127, His115, Ala97, Ser98 and Glu128) and 4 aa on AP3D1 (Lys925, Asp807, Asp695 and Arg800) was highly probable in the interaction between gp51 and BLVR domains. Three gp51 recombinant peptides were expressed and purified to validate these results: the complete domain (rgp51), the N-terminal portion (rNgp51) and the C-terminal fragment (rCgp51); and binding assays to Madin-Darby bovine kidney (MDBK) cells were then carried out with each recombinant. It was found that rNgp51 preferentially bound to MDBK cells, suggesting this domain's functional role during invasion. The rNgp51-MDBK cell interaction was sensitive to trypsin (98% reduction) and chymotrypsin treatment (80% reduction). These results highlighted that the N-terminal portion of gp51 interacted in vitro with the AP3D1 receptor and provides a plausible in silico interaction model.

Idioma originalEnglish (US)
Número de artículoe0199397
PublicaciónPLoS One
Volumen13
N.º6
DOI
EstadoPublished - jun 21 2018

Citar esto

Corredor, A. P., González, J., Baquero, L. A., Curtidor, H., Olaya-Galán, N. N., Patarroyo, M. A., & Gutiérrez, M. F. (2018). In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51. PLoS One, 13(6), [e0199397]. https://doi.org/10.1371/journal.pone.0199397
Corredor, Adriana Patricia ; González, Janneth ; Baquero, Luis Alfredo ; Curtidor, Hernando ; Olaya-Galán, Nury Nathalia ; Patarroyo, Manuel Alfonso ; Gutiérrez, María Fernanda. / In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51. En: PLoS One. 2018 ; Vol. 13, N.º 6.
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title = "In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51",
abstract = "The envelope glycoprotein 51 (gp51) is essential for bovine leukaemia virus (BLV) entry to bovine B-lymphocytes. Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools. The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. The results showed that the participation of 6 key amino acids (aa) on gp51 (Asn170, Trp127, His115, Ala97, Ser98 and Glu128) and 4 aa on AP3D1 (Lys925, Asp807, Asp695 and Arg800) was highly probable in the interaction between gp51 and BLVR domains. Three gp51 recombinant peptides were expressed and purified to validate these results: the complete domain (rgp51), the N-terminal portion (rNgp51) and the C-terminal fragment (rCgp51); and binding assays to Madin-Darby bovine kidney (MDBK) cells were then carried out with each recombinant. It was found that rNgp51 preferentially bound to MDBK cells, suggesting this domain's functional role during invasion. The rNgp51-MDBK cell interaction was sensitive to trypsin (98{\%} reduction) and chymotrypsin treatment (80{\%} reduction). These results highlighted that the N-terminal portion of gp51 interacted in vitro with the AP3D1 receptor and provides a plausible in silico interaction model.",
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Corredor, AP, González, J, Baquero, LA, Curtidor, H, Olaya-Galán, NN, Patarroyo, MA & Gutiérrez, MF 2018, 'In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51', PLoS One, vol. 13, n.º 6, e0199397. https://doi.org/10.1371/journal.pone.0199397

In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51. / Corredor, Adriana Patricia; González, Janneth; Baquero, Luis Alfredo; Curtidor, Hernando; Olaya-Galán, Nury Nathalia; Patarroyo, Manuel Alfonso; Gutiérrez, María Fernanda.

En: PLoS One, Vol. 13, N.º 6, e0199397, 21.06.2018.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51

AU - Corredor, Adriana Patricia

AU - González, Janneth

AU - Baquero, Luis Alfredo

AU - Curtidor, Hernando

AU - Olaya-Galán, Nury Nathalia

AU - Patarroyo, Manuel Alfonso

AU - Gutiérrez, María Fernanda

PY - 2018/6/21

Y1 - 2018/6/21

N2 - The envelope glycoprotein 51 (gp51) is essential for bovine leukaemia virus (BLV) entry to bovine B-lymphocytes. Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools. The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. The results showed that the participation of 6 key amino acids (aa) on gp51 (Asn170, Trp127, His115, Ala97, Ser98 and Glu128) and 4 aa on AP3D1 (Lys925, Asp807, Asp695 and Arg800) was highly probable in the interaction between gp51 and BLVR domains. Three gp51 recombinant peptides were expressed and purified to validate these results: the complete domain (rgp51), the N-terminal portion (rNgp51) and the C-terminal fragment (rCgp51); and binding assays to Madin-Darby bovine kidney (MDBK) cells were then carried out with each recombinant. It was found that rNgp51 preferentially bound to MDBK cells, suggesting this domain's functional role during invasion. The rNgp51-MDBK cell interaction was sensitive to trypsin (98% reduction) and chymotrypsin treatment (80% reduction). These results highlighted that the N-terminal portion of gp51 interacted in vitro with the AP3D1 receptor and provides a plausible in silico interaction model.

AB - The envelope glycoprotein 51 (gp51) is essential for bovine leukaemia virus (BLV) entry to bovine B-lymphocytes. Although the bovine adaptor protein 3 complex subunit delta-1 (boAP3D1) has been proposed as the potential receptor, the specific ligand-receptor interaction has not yet been completely defined and boAP3D1 receptor and gp51 3D structures have not been determined. This study was thus aimed at a functional annotation of boAP3D1 cellular adaptor protein and BLV gp51 and, proposing a reliable model for gp51-AP3D1 interaction using bioinformatics tools. The boAP3D1 receptor interaction patterns were calculated based on models of boAP3D1 receptor and gp51 complexes' 3D structures, which were constructed using homology techniques and data-driven docking strategy. The results showed that the participation of 6 key amino acids (aa) on gp51 (Asn170, Trp127, His115, Ala97, Ser98 and Glu128) and 4 aa on AP3D1 (Lys925, Asp807, Asp695 and Arg800) was highly probable in the interaction between gp51 and BLVR domains. Three gp51 recombinant peptides were expressed and purified to validate these results: the complete domain (rgp51), the N-terminal portion (rNgp51) and the C-terminal fragment (rCgp51); and binding assays to Madin-Darby bovine kidney (MDBK) cells were then carried out with each recombinant. It was found that rNgp51 preferentially bound to MDBK cells, suggesting this domain's functional role during invasion. The rNgp51-MDBK cell interaction was sensitive to trypsin (98% reduction) and chymotrypsin treatment (80% reduction). These results highlighted that the N-terminal portion of gp51 interacted in vitro with the AP3D1 receptor and provides a plausible in silico interaction model.

U2 - 10.1371/journal.pone.0199397

DO - 10.1371/journal.pone.0199397

M3 - Article

VL - 13

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 6

M1 - e0199397

ER -

Corredor AP, González J, Baquero LA, Curtidor H, Olaya-Galán NN, Patarroyo MA y otros. In silico and in vitro analysis of boAP3d1 protein interaction with bovine leukaemia virus gp51. PLoS One. 2018 jun 21;13(6). e0199397. https://doi.org/10.1371/journal.pone.0199397