Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

Zaida Araujo, Francesca Giampietro, María de los Angeles Bochichio, Andrea Palacios, Jenifer Dinis, Jaime Isern, Jacobus Henry de Waard, Elsa Rada, Rafael Borges, Carlos Fernández de Larrea, Angel Villasmil, Magnolia Vanegas, Jose Antonio Enciso-Moreno, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

8 Citas (Scopus)

Resumen

The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.
Idioma originalEnglish (US)
Páginas (desde-hasta)131-139
Número de páginas9
PublicaciónMemorias do Instituto Oswaldo Cruz
EstadoPublished - abr 1 2013

Huella dactilar

Mycobacterium tuberculosis
Pulmonary Tuberculosis
Tuberculosis
Peptides
Infection
Venezuela
Serologic Tests
Leprosy
Immunoassay
Enzyme-Linked Immunosorbent Assay
Antigens
Amino Acids

Citar esto

Araujo, Zaida ; Giampietro, Francesca ; Bochichio, María de los Angeles ; Palacios, Andrea ; Dinis, Jenifer ; Isern, Jaime ; de Waard, Jacobus Henry ; Rada, Elsa ; Borges, Rafael ; de Larrea, Carlos Fernández ; Villasmil, Angel ; Vanegas, Magnolia ; Enciso-Moreno, Jose Antonio ; Patarroyo, Manuel Alfonso. / Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection. En: Memorias do Instituto Oswaldo Cruz. 2013 ; pp. 131-139.
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title = "Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection",
abstract = "The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9{\%} and 96.2{\%}, respectively, and an Ag85A-peptide (29878) was the most specific (97.4{\%}) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3{\%} and an Ag85A-peptide (29878) was also the most specific (96.4{\%}). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5{\%} and 100{\%} sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.",
author = "Zaida Araujo and Francesca Giampietro and Bochichio, {Mar{\'i}a de los Angeles} and Andrea Palacios and Jenifer Dinis and Jaime Isern and {de Waard}, {Jacobus Henry} and Elsa Rada and Rafael Borges and {de Larrea}, {Carlos Fern{\'a}ndez} and Angel Villasmil and Magnolia Vanegas and Enciso-Moreno, {Jose Antonio} and Patarroyo, {Manuel Alfonso}",
year = "2013",
month = "4",
day = "1",
language = "English (US)",
pages = "131--139",
journal = "Memorias do Instituto Oswaldo Cruz",
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Araujo, Z, Giampietro, F, Bochichio, MDLA, Palacios, A, Dinis, J, Isern, J, de Waard, JH, Rada, E, Borges, R, de Larrea, CF, Villasmil, A, Vanegas, M, Enciso-Moreno, JA & Patarroyo, MA 2013, 'Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection', Memorias do Instituto Oswaldo Cruz, pp. 131-139.

Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection. / Araujo, Zaida; Giampietro, Francesca; Bochichio, María de los Angeles; Palacios, Andrea; Dinis, Jenifer; Isern, Jaime; de Waard, Jacobus Henry; Rada, Elsa; Borges, Rafael; de Larrea, Carlos Fernández; Villasmil, Angel; Vanegas, Magnolia; Enciso-Moreno, Jose Antonio; Patarroyo, Manuel Alfonso.

En: Memorias do Instituto Oswaldo Cruz, 01.04.2013, p. 131-139.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Immunologic evaluation and validation of methods using synthetic peptides derived from Mycobacterium tuberculosis for the diagnosis of tuberculosis infection

AU - Araujo, Zaida

AU - Giampietro, Francesca

AU - Bochichio, María de los Angeles

AU - Palacios, Andrea

AU - Dinis, Jenifer

AU - Isern, Jaime

AU - de Waard, Jacobus Henry

AU - Rada, Elsa

AU - Borges, Rafael

AU - de Larrea, Carlos Fernández

AU - Villasmil, Angel

AU - Vanegas, Magnolia

AU - Enciso-Moreno, Jose Antonio

AU - Patarroyo, Manuel Alfonso

PY - 2013/4/1

Y1 - 2013/4/1

N2 - The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.

AB - The goal of this study was to demonstrate the usefulness of an enzyme-linked immunosorbent assay (ELISA) for the serodiagnosis of pulmonary tuberculosis (PTB) and extrapulmonary TB (EPTB). This assay used 20 amino acid-long, non-overlapped synthetic peptides that spanned the complete Mycobacterium tuberculosis ESAT-6 and Ag85A sequences. The validation cohort consisted of 1,102 individuals who were grouped into the following five diagnostic groups: 455 patients with PTB, 60 patients with EPTB, 40 individuals with non-EPTB, 33 individuals with leprosy and 514 healthy controls. For the PTB group, two ESAT-6 peptides (12033 and 12034) had the highest sensitivity levels of 96.9% and 96.2%, respectively, and an Ag85A-peptide (29878) was the most specific (97.4%) in the PTB groups. For the EPTB group, two Ag85A peptides (11005 and 11006) were observed to have a sensitivity of 98.3% and an Ag85A-peptide (29878) was also the most specific (96.4%). When combinations of peptides were used, such as 12033 and 12034 or 11005 and 11006, 99.5% and 100% sensitivities in the PTB and EPTB groups were observed, respectively. In conclusion, for a cohort that consists entirely of individuals from Venezuela, a multi-antigen immunoassay using highly sensitive ESAT-6 and Ag85A peptides alone and in combination could be used to more rapidly diagnose PTB and EPTB infection.

M3 - Article

SP - 131

EP - 139

JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

SN - 0074-0276

ER -