Immunogenetics of primary Sjögren's syndrome in Colombians

Juan Manuel Anaya, Ruben D. Mantilla, Paula A. Correa

Resultado de la investigación: Contribución a RevistaArtículo

22 Citas (Scopus)

Resumen

OBJECTIVE: Data concerning the immunogenetic characteristics of primary Sjögren's syndrome (SS) in Latin-Americans are scarce. A research project centered on primary SS in Colombians was initiated in January 1996 to better define these characteristics. METHODS: TAP, HLA, IL-10, and microsatellites on 6p21.3 genotyping was performed by polymerase chain reaction techniques. Immunohistochemistry for Bcl-2 antagonist/killer (Bak) was performed. Autoantibodies and serum level of cytokines (IL-10, TNF-α, IFN-γ, IL-4, and IL-12p70) were determined by enzyme-linked immunosorbent assay. RESULTS: The HLA-DRB1*0301-DQB1*0201 haplotype was associated with disease (OR = 4.3, 95% CI: 1.6 to 11.9, P = 0.002), with a more severe histopathologic picture, and with the presence of anti-Ro and anti-La antibodies. D6S439 microsatellite polymorphism was associated with primary SS in an HLA-independent manner. The most likely gene related to the D6S439 chromosomal location appears to be BAK-1, which codes for Bak protein, expressed in salivary gland's infiltrate from patients with primary SS but not in controls. IL-10 and IFN-γ concentrations were significantly higher in patients than in controls (P <0.01). IL-10 correlated with titers of IgA rheumatoid factor, anti-Ro, and anti-La antibodies, and with the severity of lymphocytic infiltrate (r > 0.3, P <0.04). Patients who produced high IL-10 levels had significantly more episodes of cutaneous vasculitis and a higher proportion the IL-10.G9 allele. CONCLUSIONS: The HLA-DRB1*0301- DQB1*0201 haplotype and IL-10 participate in the histopathological progression of SS, autoantibody production, and clinical manifestations. Bak protein and its gene polymorphism may participate in the pathology and susceptibility of disease. HLA and cytokine (IL-10 and IFN-γ) manipulation may be helpful in treating patients with primary SS. © 2005 Elsevier Inc. All rights reserved.
Idioma originalEnglish (US)
Páginas (desde-hasta)735-743
Número de páginas9
PublicaciónSeminars in Arthritis and Rheumatism
DOI
EstadoPublished - abr 1 2005

Huella dactilar

Immunogenetics
Sjogren's Syndrome
Interleukin-10
Microsatellite Repeats
Autoantibodies
Haplotypes
Anti-Idiotypic Antibodies
Cytokines
Rheumatoid Factor
Disease Susceptibility
Vasculitis
Salivary Glands
Interleukin-4
Immunoglobulin A
Proteins
Tumor Necrosis Factor-alpha
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry
Alleles
Pathology

Citar esto

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title = "Immunogenetics of primary Sj{\"o}gren's syndrome in Colombians",
abstract = "OBJECTIVE: Data concerning the immunogenetic characteristics of primary Sj{\"o}gren's syndrome (SS) in Latin-Americans are scarce. A research project centered on primary SS in Colombians was initiated in January 1996 to better define these characteristics. METHODS: TAP, HLA, IL-10, and microsatellites on 6p21.3 genotyping was performed by polymerase chain reaction techniques. Immunohistochemistry for Bcl-2 antagonist/killer (Bak) was performed. Autoantibodies and serum level of cytokines (IL-10, TNF-α, IFN-γ, IL-4, and IL-12p70) were determined by enzyme-linked immunosorbent assay. RESULTS: The HLA-DRB1*0301-DQB1*0201 haplotype was associated with disease (OR = 4.3, 95{\%} CI: 1.6 to 11.9, P = 0.002), with a more severe histopathologic picture, and with the presence of anti-Ro and anti-La antibodies. D6S439 microsatellite polymorphism was associated with primary SS in an HLA-independent manner. The most likely gene related to the D6S439 chromosomal location appears to be BAK-1, which codes for Bak protein, expressed in salivary gland's infiltrate from patients with primary SS but not in controls. IL-10 and IFN-γ concentrations were significantly higher in patients than in controls (P <0.01). IL-10 correlated with titers of IgA rheumatoid factor, anti-Ro, and anti-La antibodies, and with the severity of lymphocytic infiltrate (r > 0.3, P <0.04). Patients who produced high IL-10 levels had significantly more episodes of cutaneous vasculitis and a higher proportion the IL-10.G9 allele. CONCLUSIONS: The HLA-DRB1*0301- DQB1*0201 haplotype and IL-10 participate in the histopathological progression of SS, autoantibody production, and clinical manifestations. Bak protein and its gene polymorphism may participate in the pathology and susceptibility of disease. HLA and cytokine (IL-10 and IFN-γ) manipulation may be helpful in treating patients with primary SS. {\circledC} 2005 Elsevier Inc. All rights reserved.",
author = "Anaya, {Juan Manuel} and Mantilla, {Ruben D.} and Correa, {Paula A.}",
year = "2005",
month = "4",
day = "1",
doi = "10.1016/j.semarthrit.2004.11.008",
language = "English (US)",
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journal = "Seminars in Arthritis and Rheumatism",
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Immunogenetics of primary Sjögren's syndrome in Colombians. / Anaya, Juan Manuel; Mantilla, Ruben D.; Correa, Paula A.

En: Seminars in Arthritis and Rheumatism, 01.04.2005, p. 735-743.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Immunogenetics of primary Sjögren's syndrome in Colombians

AU - Anaya, Juan Manuel

AU - Mantilla, Ruben D.

AU - Correa, Paula A.

PY - 2005/4/1

Y1 - 2005/4/1

N2 - OBJECTIVE: Data concerning the immunogenetic characteristics of primary Sjögren's syndrome (SS) in Latin-Americans are scarce. A research project centered on primary SS in Colombians was initiated in January 1996 to better define these characteristics. METHODS: TAP, HLA, IL-10, and microsatellites on 6p21.3 genotyping was performed by polymerase chain reaction techniques. Immunohistochemistry for Bcl-2 antagonist/killer (Bak) was performed. Autoantibodies and serum level of cytokines (IL-10, TNF-α, IFN-γ, IL-4, and IL-12p70) were determined by enzyme-linked immunosorbent assay. RESULTS: The HLA-DRB1*0301-DQB1*0201 haplotype was associated with disease (OR = 4.3, 95% CI: 1.6 to 11.9, P = 0.002), with a more severe histopathologic picture, and with the presence of anti-Ro and anti-La antibodies. D6S439 microsatellite polymorphism was associated with primary SS in an HLA-independent manner. The most likely gene related to the D6S439 chromosomal location appears to be BAK-1, which codes for Bak protein, expressed in salivary gland's infiltrate from patients with primary SS but not in controls. IL-10 and IFN-γ concentrations were significantly higher in patients than in controls (P <0.01). IL-10 correlated with titers of IgA rheumatoid factor, anti-Ro, and anti-La antibodies, and with the severity of lymphocytic infiltrate (r > 0.3, P <0.04). Patients who produced high IL-10 levels had significantly more episodes of cutaneous vasculitis and a higher proportion the IL-10.G9 allele. CONCLUSIONS: The HLA-DRB1*0301- DQB1*0201 haplotype and IL-10 participate in the histopathological progression of SS, autoantibody production, and clinical manifestations. Bak protein and its gene polymorphism may participate in the pathology and susceptibility of disease. HLA and cytokine (IL-10 and IFN-γ) manipulation may be helpful in treating patients with primary SS. © 2005 Elsevier Inc. All rights reserved.

AB - OBJECTIVE: Data concerning the immunogenetic characteristics of primary Sjögren's syndrome (SS) in Latin-Americans are scarce. A research project centered on primary SS in Colombians was initiated in January 1996 to better define these characteristics. METHODS: TAP, HLA, IL-10, and microsatellites on 6p21.3 genotyping was performed by polymerase chain reaction techniques. Immunohistochemistry for Bcl-2 antagonist/killer (Bak) was performed. Autoantibodies and serum level of cytokines (IL-10, TNF-α, IFN-γ, IL-4, and IL-12p70) were determined by enzyme-linked immunosorbent assay. RESULTS: The HLA-DRB1*0301-DQB1*0201 haplotype was associated with disease (OR = 4.3, 95% CI: 1.6 to 11.9, P = 0.002), with a more severe histopathologic picture, and with the presence of anti-Ro and anti-La antibodies. D6S439 microsatellite polymorphism was associated with primary SS in an HLA-independent manner. The most likely gene related to the D6S439 chromosomal location appears to be BAK-1, which codes for Bak protein, expressed in salivary gland's infiltrate from patients with primary SS but not in controls. IL-10 and IFN-γ concentrations were significantly higher in patients than in controls (P <0.01). IL-10 correlated with titers of IgA rheumatoid factor, anti-Ro, and anti-La antibodies, and with the severity of lymphocytic infiltrate (r > 0.3, P <0.04). Patients who produced high IL-10 levels had significantly more episodes of cutaneous vasculitis and a higher proportion the IL-10.G9 allele. CONCLUSIONS: The HLA-DRB1*0301- DQB1*0201 haplotype and IL-10 participate in the histopathological progression of SS, autoantibody production, and clinical manifestations. Bak protein and its gene polymorphism may participate in the pathology and susceptibility of disease. HLA and cytokine (IL-10 and IFN-γ) manipulation may be helpful in treating patients with primary SS. © 2005 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.semarthrit.2004.11.008

DO - 10.1016/j.semarthrit.2004.11.008

M3 - Article

SP - 735

EP - 743

JO - Seminars in Arthritis and Rheumatism

JF - Seminars in Arthritis and Rheumatism

SN - 0049-0172

ER -