Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers

Isabelle Peyrottes, Valerie Navarro, Alejandro Ondo-Mendez, Didier Marcellin, Laurent Bellanger, Robert Marsault, Sabine Lindenthal, Francette Ettore, Jacques Darcourt, Thierry Pourcher

Resultado de la investigación: Contribución a RevistaArtículo

34 Citas (Scopus)

Resumen

Objective: The active transport of iodide into thyroid cells is mediated by the Na+/I- symporter (NIS) located in the basolateral membrane. Strong intracellular staining with anti-NIS antibodies has been reported in thyroid and breast cancers. Our initial objective was to screen tumour samples for intracellular NIS staining and then to study the mechanisms underlying the altered subcellular localization of the transporters. Methods: Immunostaining using three different anti-NIS antibodies was performed on paraffin-embedded tissue sections from 93 thyroid or breast cancers. Western blot experiments were carried out to determine the amount of NIS protein in 20 samples. Results: Using three different anti-NIS antibodies, we observed intracellular staining in a majority of thyroid tumour samples. Control immunohistochemistry and western blot experiments indicated that this intracellular staining was due to non-specific binding of the antibodies. In breast tumours, very weak intracellular staining was observed in some samples. Western blot experiments suggest that this labelling is also non-specific. Conclusions: Our results strongly indicate that the NIS protein level is low in thyroid and breast cancers and that the intracellular staining obtained with anti-NIS antibodies corresponds to a non-specific signal. Accordingly, to increase the efficiency of radiotherapy for thyroid cancers and to enable the use of radioiodine in the diagnosis and therapy of breast tumours, improving NIS targeting to the plasma membrane will not be sufficient. Instead, increasing the expression level of NIS should remain the major goal of this field. © 2009 European Society of Endocrinology.
Idioma originalEnglish (US)
Páginas (desde-hasta)215-225
Número de páginas11
PublicaciónEuropean Journal of Endocrinology
Volumen160
N.º2
DOI
EstadoPublished - may 19 2009
Publicado de forma externa

Huella dactilar

Symporters
Thyroid Neoplasms
Breast Neoplasms
Staining and Labeling
Antibodies
Western Blotting
Thyroid Gland
sodium-iodide symporter
Active Biological Transport
Iodides
Paraffin
Neoplasms
Proteins
Radiotherapy
Immunohistochemistry
Cell Membrane

Citar esto

Peyrottes, Isabelle ; Navarro, Valerie ; Ondo-Mendez, Alejandro ; Marcellin, Didier ; Bellanger, Laurent ; Marsault, Robert ; Lindenthal, Sabine ; Ettore, Francette ; Darcourt, Jacques ; Pourcher, Thierry. / Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers. En: European Journal of Endocrinology. 2009 ; Vol. 160, N.º 2. pp. 215-225.
@article{55f6650195fd4656823376daf697a280,
title = "Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers",
abstract = "Objective: The active transport of iodide into thyroid cells is mediated by the Na+/I- symporter (NIS) located in the basolateral membrane. Strong intracellular staining with anti-NIS antibodies has been reported in thyroid and breast cancers. Our initial objective was to screen tumour samples for intracellular NIS staining and then to study the mechanisms underlying the altered subcellular localization of the transporters. Methods: Immunostaining using three different anti-NIS antibodies was performed on paraffin-embedded tissue sections from 93 thyroid or breast cancers. Western blot experiments were carried out to determine the amount of NIS protein in 20 samples. Results: Using three different anti-NIS antibodies, we observed intracellular staining in a majority of thyroid tumour samples. Control immunohistochemistry and western blot experiments indicated that this intracellular staining was due to non-specific binding of the antibodies. In breast tumours, very weak intracellular staining was observed in some samples. Western blot experiments suggest that this labelling is also non-specific. Conclusions: Our results strongly indicate that the NIS protein level is low in thyroid and breast cancers and that the intracellular staining obtained with anti-NIS antibodies corresponds to a non-specific signal. Accordingly, to increase the efficiency of radiotherapy for thyroid cancers and to enable the use of radioiodine in the diagnosis and therapy of breast tumours, improving NIS targeting to the plasma membrane will not be sufficient. Instead, increasing the expression level of NIS should remain the major goal of this field. {\circledC} 2009 European Society of Endocrinology.",
author = "Isabelle Peyrottes and Valerie Navarro and Alejandro Ondo-Mendez and Didier Marcellin and Laurent Bellanger and Robert Marsault and Sabine Lindenthal and Francette Ettore and Jacques Darcourt and Thierry Pourcher",
year = "2009",
month = "5",
day = "19",
doi = "10.1530/EJE-08-0505",
language = "English (US)",
volume = "160",
pages = "215--225",
journal = "European Journal of Endocrinology",
issn = "0804-4643",
publisher = "BioScientifica Ltd.",
number = "2",

}

Peyrottes, I, Navarro, V, Ondo-Mendez, A, Marcellin, D, Bellanger, L, Marsault, R, Lindenthal, S, Ettore, F, Darcourt, J & Pourcher, T 2009, 'Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers', European Journal of Endocrinology, vol. 160, n.º 2, pp. 215-225. https://doi.org/10.1530/EJE-08-0505

Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers. / Peyrottes, Isabelle; Navarro, Valerie; Ondo-Mendez, Alejandro; Marcellin, Didier; Bellanger, Laurent; Marsault, Robert; Lindenthal, Sabine; Ettore, Francette; Darcourt, Jacques; Pourcher, Thierry.

En: European Journal of Endocrinology, Vol. 160, N.º 2, 19.05.2009, p. 215-225.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Immunoanalysis indicates that the sodium iodide symporter is not overexpressed in intracellular compartments in thyroid and breast cancers

AU - Peyrottes, Isabelle

AU - Navarro, Valerie

AU - Ondo-Mendez, Alejandro

AU - Marcellin, Didier

AU - Bellanger, Laurent

AU - Marsault, Robert

AU - Lindenthal, Sabine

AU - Ettore, Francette

AU - Darcourt, Jacques

AU - Pourcher, Thierry

PY - 2009/5/19

Y1 - 2009/5/19

N2 - Objective: The active transport of iodide into thyroid cells is mediated by the Na+/I- symporter (NIS) located in the basolateral membrane. Strong intracellular staining with anti-NIS antibodies has been reported in thyroid and breast cancers. Our initial objective was to screen tumour samples for intracellular NIS staining and then to study the mechanisms underlying the altered subcellular localization of the transporters. Methods: Immunostaining using three different anti-NIS antibodies was performed on paraffin-embedded tissue sections from 93 thyroid or breast cancers. Western blot experiments were carried out to determine the amount of NIS protein in 20 samples. Results: Using three different anti-NIS antibodies, we observed intracellular staining in a majority of thyroid tumour samples. Control immunohistochemistry and western blot experiments indicated that this intracellular staining was due to non-specific binding of the antibodies. In breast tumours, very weak intracellular staining was observed in some samples. Western blot experiments suggest that this labelling is also non-specific. Conclusions: Our results strongly indicate that the NIS protein level is low in thyroid and breast cancers and that the intracellular staining obtained with anti-NIS antibodies corresponds to a non-specific signal. Accordingly, to increase the efficiency of radiotherapy for thyroid cancers and to enable the use of radioiodine in the diagnosis and therapy of breast tumours, improving NIS targeting to the plasma membrane will not be sufficient. Instead, increasing the expression level of NIS should remain the major goal of this field. © 2009 European Society of Endocrinology.

AB - Objective: The active transport of iodide into thyroid cells is mediated by the Na+/I- symporter (NIS) located in the basolateral membrane. Strong intracellular staining with anti-NIS antibodies has been reported in thyroid and breast cancers. Our initial objective was to screen tumour samples for intracellular NIS staining and then to study the mechanisms underlying the altered subcellular localization of the transporters. Methods: Immunostaining using three different anti-NIS antibodies was performed on paraffin-embedded tissue sections from 93 thyroid or breast cancers. Western blot experiments were carried out to determine the amount of NIS protein in 20 samples. Results: Using three different anti-NIS antibodies, we observed intracellular staining in a majority of thyroid tumour samples. Control immunohistochemistry and western blot experiments indicated that this intracellular staining was due to non-specific binding of the antibodies. In breast tumours, very weak intracellular staining was observed in some samples. Western blot experiments suggest that this labelling is also non-specific. Conclusions: Our results strongly indicate that the NIS protein level is low in thyroid and breast cancers and that the intracellular staining obtained with anti-NIS antibodies corresponds to a non-specific signal. Accordingly, to increase the efficiency of radiotherapy for thyroid cancers and to enable the use of radioiodine in the diagnosis and therapy of breast tumours, improving NIS targeting to the plasma membrane will not be sufficient. Instead, increasing the expression level of NIS should remain the major goal of this field. © 2009 European Society of Endocrinology.

U2 - 10.1530/EJE-08-0505

DO - 10.1530/EJE-08-0505

M3 - Article

VL - 160

SP - 215

EP - 225

JO - European Journal of Endocrinology

JF - European Journal of Endocrinology

SN - 0804-4643

IS - 2

ER -