Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

David Martínez; Carolina Hernández; Marina Muñoz; Yulieth Armesto; Andres Cuervo; Juan David Ramírez

doi:10.3389/fevo.2020.602190

Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

Título traducido de la contribución: Identificación de las especies de Aedes (Diptera: Culicidae) y de los arbovirus que circulan en Arauca, este de Colombia

David Martínez, Carolina Hernández, Marina Muñoz, Yulieth Armesto, Andres Cuervo, Juan David Ramírez

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

9 Citas (Scopus)

Resumen

La identificación de las especies de vectores y su infección natural con arbovirus permite obtener datos importantes para el control de su transmisión. Sin embargo, para la región oriental de Colombia, esta información es limitada. Por lo tanto, en este estudio se identificaron morfológica y molecularmente las especies del género Aedes y la detección de arbovirus (Dengue, Chikungunya, Zika y Mayaro) en mosquitos hembra (individualmente) presentes en tres municipios (Saravena, Arauquita y Tame) mediante la amplificación del material genético por RT-PCR (reacción en cadena de la polimerasa con transcriptasa inversa) en el departamento de Arauca, oriente colombiano. Se detectaron inconsistencias entre la identificación morfológica y la molecular en 13 individuos con Aedes albopictus determinados inicialmente como Aedes aegypti con base en la morfología (n = 13). La identificación molecular mostró la presencia simultánea de A. aegypti (n = 111) y A. albopictus (n = 58) en los municipios urbanos de Saravena y Arauquita. Estos individuos estaban infectados de forma natural por el virus del dengue tipo 1 (DENV-1) y el virus del chikungunya (CHIKV). El arbovirus más frecuente fue el DENV-1 con una tasa de infección del 40,7% (11/27) para A. aegypti y del 39,7% (23/58) para A. albopictus, seguido del CHIKV con una tasa de infección del 1,8% para A. aegypti (2/111) y del 6,9% para A. albopictus (4/58). Además, se obtuvo una infección mixta de DENV-1 y CHIKV en el 4,5% de A. aegypti (5/111). No se detectaron infecciones por el virus Zika (ZIKV) ni por el virus Mayaro (MAYV). Este estudio ha revelado que el código de barras (fragmento del gen COI) es un método exitoso para identificar las especies de Aedes. Además, se recomienda el procesamiento individual de los insectos como estrategia más precisa para la detección de arbovirus, ya que se obtiene la tasa de infección y también es posible la coinfección entre DENV-1 y CHIKV.

Título traducido de la contribución	Identificación de las especies de Aedes (Diptera: Culicidae) y de los arbovirus que circulan en Arauca, este de Colombia
Idioma original	Inglés estadounidense
Número de artículo	602190
Publicación	Frontiers in Ecology and Evolution
Volumen	8
DOI	https://doi.org/10.3389/fevo.2020.602190
Estado	Publicada - nov. 26 2020

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Ecología, evolución, comportamiento y sistemática
Ecología

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title = "Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia",

abstract = "The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus Aedes and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with Aedes albopictus initially determined as Aedes aegypti based on morphology (n = 13). Molecular identification showed the simultaneous presence of A. aegypti (n = 111) and A. albopictus (n = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for A. aegypti and 39.7% (23/58) for A. albopictus, which was followed by CHIKV with an infection rate of 1.8% for A. aegypti (2/111) and 6.9% for A. albopictus (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of A. aegypti (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying Aedes species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.",

author = "David Mart{\'i}nez and Carolina Hern{\'a}ndez and Marina Mu{\~n}oz and Yulieth Armesto and Andres Cuervo and Ram{\'i}rez, {Juan David}",

note = "Funding Information: Funding. We would like to thank the Direcci?n de Investigaci?n e Innovaci?n from Universidad del Rosario for funding this study and providing the editing English service of ENAGO and cover the publication fees. Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2020 Mart{\'i}nez, Hern{\'a}ndez, Mu{\~n}oz, Armesto, Cuervo and Ram{\'i}rez. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

year = "2020",

month = nov,

day = "26",

doi = "10.3389/fevo.2020.602190",

language = "English (US)",

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T1 - Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

AU - Martínez, David

AU - Hernández, Carolina

AU - Muñoz, Marina

AU - Armesto, Yulieth

AU - Cuervo, Andres

AU - Ramírez, Juan David

N1 - Funding Information: Funding. We would like to thank the Direcci?n de Investigaci?n e Innovaci?n from Universidad del Rosario for funding this study and providing the editing English service of ENAGO and cover the publication fees. Publisher Copyright: © Copyright © 2020 Martínez, Hernández, Muñoz, Armesto, Cuervo and Ramírez. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/11/26

Y1 - 2020/11/26

N2 - The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus Aedes and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with Aedes albopictus initially determined as Aedes aegypti based on morphology (n = 13). Molecular identification showed the simultaneous presence of A. aegypti (n = 111) and A. albopictus (n = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for A. aegypti and 39.7% (23/58) for A. albopictus, which was followed by CHIKV with an infection rate of 1.8% for A. aegypti (2/111) and 6.9% for A. albopictus (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of A. aegypti (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying Aedes species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.

AB - The identification of vector species and their natural infection with arboviruses results in important data for the control of their transmission. However, for the eastern region of Colombia, this information is limited. Therefore, this study morphologically and molecularly identified species of the genus Aedes and the detection of arboviruses (Dengue, Chikungunya, Zika, and Mayaro) in female mosquitoes (individually) present in three municipalities (Saravena, Arauquita, and Tame) by amplifying the genetic material using RT-PCR (reverse transcriptase polymerase chain reaction) in the department of Arauca, eastern Colombia. Inconsistencies between morphological and molecular identification were detected in 13 individuals with Aedes albopictus initially determined as Aedes aegypti based on morphology (n = 13). Molecular identification showed the simultaneous presence of A. aegypti (n = 111) and A. albopictus (n = 58) in the urban municipalities of Saravena and Arauquita. These individuals were naturally infected with Dengue virus type 1 (DENV-1) and Chikungunya virus (CHIKV). The most frequent arbovirus was DENV-1 with an infection rate of 40.7% (11/27) for A. aegypti and 39.7% (23/58) for A. albopictus, which was followed by CHIKV with an infection rate of 1.8% for A. aegypti (2/111) and 6.9% for A. albopictus (4/58). Additionally, a mixed infection of DENV-1 and CHIKV was obtained in 4.5% of A. aegypti (5/111). Zika virus (ZIKV) and Mayaro virus (MAYV) infections were not detected. This study found that barcoding (fragment gene COI) is a successful method for identifying Aedes species. Additionally, we recommend the individual processing of insects as a more accurate strategy for arboviruses detection since the infection rate is obtained and co-infection between DENV-1 and CHIKV is also possible.

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Identification of Aedes (Diptera: Culicidae) Species and Arboviruses Circulating in Arauca, Eastern Colombia

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