Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein

Carolina Saravia, Paola Martinez, Diana S. Granados, Carolina Lopez, Claudia Reyes, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

8 Citas (Scopus)

Resumen

Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. © 2008 Elsevier Inc. All rights reserved.
Idioma originalEnglish (US)
Páginas (desde-hasta)1279-1283
Número de páginas5
PublicaciónBiochemical and Biophysical Research Communications
DOI
EstadoPublished - dic 26 2008

Huella dactilar

Plasmodium vivax
Epitopes
Carrier Proteins
Molecules
Peptides
HLA-DR7 Antigen
HLA-DR1 Antigen
HLA-DR4 Antigen
Vivax Malaria
Synthetic Vaccines
HLA-DR Antigens
Plasmodium Duffy antigen binding protein
Enzyme-Linked Immunosorbent Assay
Assays

Citar esto

Saravia, Carolina ; Martinez, Paola ; Granados, Diana S. ; Lopez, Carolina ; Reyes, Claudia ; Patarroyo, Manuel A. / Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein. En: Biochemical and Biophysical Research Communications. 2008 ; pp. 1279-1283.
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abstract = "Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50{\%}) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. {\circledC} 2008 Elsevier Inc. All rights reserved.",
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Identification and evaluation of universal epitopes in Plasmodium vivax Duffy binding protein. / Saravia, Carolina; Martinez, Paola; Granados, Diana S.; Lopez, Carolina; Reyes, Claudia; Patarroyo, Manuel A.

En: Biochemical and Biophysical Research Communications, 26.12.2008, p. 1279-1283.

Resultado de la investigación: Contribución a RevistaArtículo

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AU - Saravia, Carolina

AU - Martinez, Paola

AU - Granados, Diana S.

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AU - Reyes, Claudia

AU - Patarroyo, Manuel A.

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AB - Selected PvDBP-derived synthetic peptides were tested in competition assays with HLA molecules in order to identify and evaluate their binding to a wide range of MHC class II molecules. Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306-318) and was detected by a conventional ELISA. Thus, one epitope for the HLA-DR1 molecule, two epitopes for the HLA-DR4 molecule, six epitopes for the HLA-DR7 molecule and three epitopes for the HLA-DR11 molecule displaying a high binding percentage (above 50%) were experimentally obtained. The in vitro results were compared with the epitope prediction results. Two peptides behaved as universal epitopes since they bound to a larger number of HLA-DR molecules. Given that these peptides are located in the conserved PvDBP region II, they could be considered good candidates to be included in the design of a synthetic vaccine against Plasmodium vivax malaria. © 2008 Elsevier Inc. All rights reserved.

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