Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Luisa Matheus; G. Eric Blair

doi:10.1016/j.bbaexp.2003.08.010

Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

Luisa Matheus
, G. Eric Blair

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

Resumen

The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

Idioma original	Inglés estadounidense
Páginas (desde-hasta)	47-53
Número de páginas	7
Publicación	Biochimica et Biophysica Acta - Gene Structure and Expression
Volumen	1630
N.º	1
DOI	https://doi.org/10.1016/j.bbaexp.2003.08.010
Estado	Publicada - oct. 20 2003
Publicado de forma externa	Sí

Áreas temáticas de ASJC Scopus

Biología estructural
Biofísica
Bioquímica
Genética

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10.1016/j.bbaexp.2003.08.010

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title = "Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein",

abstract = "The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.",

author = "Luisa Matheus and Blair, \{G. Eric\}",

note = "Funding Information: This research was funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland. We thank Dr. A. Asipu for helpful advice and discussion. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.bbaexp.2003.08.010",

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T1 - Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

AU - Matheus, Luisa

AU - Blair, G. Eric

N1 - Funding Information: This research was funded by the Multiple Sclerosis Society of Great Britain and Northern Ireland. We thank Dr. A. Asipu for helpful advice and discussion. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2003/10/20

Y1 - 2003/10/20

N2 - The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

AB - The myelin basic proteins (MBPs) are the major proteins of the myelin membrane. Multiple MBP mRNAs and protein isoforms are generated by alternative RNA splicing. Here we describe the isolation and characterisation of a cDNA clone encoding a 17-kDa MBP isoform from the rat (Rattus norvegicus). The isoform is a 158-amino acid protein consisting of exons 1, 3, 4, 6 and 7 of the MBP gene. RT-PCR analysis of brain mRNA showed that transcripts encoding the 17-kDa isoform were expressed at higher levels early in post-natal development, up to 7 days post-partum.

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DO - 10.1016/j.bbaexp.2003.08.010

M3 - Research Article

C2 - 14580679

AN - SCOPUS:0142027076

SN - 0167-4781

VL - 1630

SP - 47

EP - 53

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 1

ER -

Identification and characterisation of a cDNA encoding a 17-kDa isoform of rat myelin basic protein

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