Cryptococcus neoformans causing meningoencephalitis in adults and a child from Lima, Peru: genotypic diversity and antifungal susceptibility

Carolina Firacative; Natalia Zuluaga-Puerto; José Guevara

doi:10.3390/jof8121306

Cryptococcus neoformans causing meningoencephalitis in adults and a child from Lima, Peru: genotypic diversity and antifungal susceptibility

Título traducido de la contribución: Cryptococcus neoformans causante de meningoencefalitis en adultos y un niño de Lima (Perú): diversidad genotípica y susceptibilidad a los antifúngicos

Carolina Firacative, Natalia Zuluaga-Puerto, José Guevara

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

1 Cita (Scopus)

Resumen

La criptococosis, causada predominantemente por Cryptococcus neoformans, es una infección oportunista potencialmente mortal que suele afectar al sistema nervioso central de pacientes inmunodeprimidos. A nivel mundial, esta micosis es responsable de casi el 20% de las muertes relacionadas con el SIDA, y en países como Perú, su incidencia sigue siendo alta, principalmente debido al aumento anual de nuevos casos de infección por VIH. Este estudio tuvo como objetivo establecer la diversidad genotípica y la susceptibilidad antifúngica de aislamientos de C. neoformans causantes de meningoencefalitis en 25 adultos y una niña de 9 años con VIH y otros factores de riesgo de Lima, Perú. Para identificar el genotipo de los aislados se aplicó la tipificación de secuencias multilocus, y para establecer la susceptibilidad de los aislados a seis antifúngicos se utilizó una microdilución en caldo YeastOne®. De los aislados, 19 se identificaron como tipo molecular VNI, y siete como VNII, agrupados en ocho y tres tipos de secuencia, respectivamente, lo que demuestra que la población estudiada era muy diversa. La mayoría de los aislados eran sensibles a todos los antifúngicos probados. Sin embargo, los aislados VNI fueron menos susceptibles a fluconazol, itraconazol y voriconazol que los aislados VNII (p> 0,05). Este estudio aporta datos sobre la epidemiología molecular y el perfil de susceptibilidad antifúngica del agente etiológico más común de la criptococosis, destacando un caso pediátrico, algo poco frecuente entre las infecciones criptocócicas.

Título traducido de la contribución	Cryptococcus neoformans causante de meningoencefalitis en adultos y un niño de Lima (Perú): diversidad genotípica y susceptibilidad a los antifúngicos
Idioma original	Inglés estadounidense
Publicación	Journal of Fungi
Volumen	8
N.º	12
DOI	https://doi.org/10.3390/jof8121306
Estado	Publicada - dic. 16 2022

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@article{477e701da464455694da7a26375af239,

title = "Cryptococcus neoformans causing meningoencephalitis in adults and a child from Lima, Peru: genotypic diversity and antifungal susceptibility",

abstract = "Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C. neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne{\textregistered} broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p> 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.",

author = "Carolina Firacative and Natalia Zuluaga-Puerto and Jos{\'e} Guevara",

year = "2022",

month = dec,

day = "16",

doi = "10.3390/jof8121306",

language = "English (US)",

volume = "8",

journal = "Journal of Fungi",

issn = "2309-608X",

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TY - JOUR

T1 - Cryptococcus neoformans causing meningoencephalitis in adults and a child from Lima, Peru: genotypic diversity and antifungal susceptibility

AU - Firacative, Carolina

AU - Zuluaga-Puerto, Natalia

AU - Guevara, José

PY - 2022/12/16

Y1 - 2022/12/16

N2 - Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C. neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne® broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p> 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.

AB - Cryptococcosis, caused predominantly by Cryptococcus neoformans, is a potentially fatal, opportunistic infection that commonly affects the central nervous system of immunocompromised patients. Globally, this mycosis is responsible for almost 20% of AIDS-related deaths, and in countries like Peru, its incidence remains high, mostly due to the annual increase in new cases of HIV infection. This study aimed to establish the genotypic diversity and antifungal susceptibility of C. neoformans isolates causing meningoencephalitis in 25 adults and a 9-year-old girl with HIV and other risk factors from Lima, Peru. To identify the genotype of the isolates, multilocus sequence typing was applied, and to establish the susceptibility of the isolates to six antifungals, a YeastOne® broth microdilution was used. From the isolates, 19 were identified as molecular type VNI, and seven as VNII, grouped in eight and three sequence types, respectively, which shows that the studied population was highly diverse. Most isolates were susceptible to all antifungals tested. However, VNI isolates were less susceptible to fluconazole, itraconazole and voriconazole than VNII isolates (p> 0.05). This study contributes data on the molecular epidemiology and the antifungal susceptibility profile of the most common etiological agent of cryptococcosis, highlighting a pediatric case, something which is rare among cryptococcal infection.

U2 - 10.3390/jof8121306

DO - 10.3390/jof8121306

M3 - Research Article

C2 - 36547639

SN - 2309-608X

VL - 8

JO - Journal of Fungi

JF - Journal of Fungi

IS - 12

ER -