Human Pathology

Semir Vranic, Caterina Marchiò, Isabella Castellano, Cristina Botta, Maria Stella Scalzo, Ryan P. Bender, Cesar Payan-Gomez, Ludovica Verdun Di Cantogno, Patrizia Gugliotta, Fabrizio Tondat, Paola Francia Di Celle, Sara Mariani, Zoran Gatalica, Anna Sapino

Resultado de la investigación: Tipos de Contribuciónes en ConferenciaPaper

17 Citas (Scopus)

Resumen

© 2015 Elsevier Inc.Summary Despite the marked improvement in the understanding of molecular mechanisms and classification of apocrine carcinoma, little is known about its specific molecular genetic alterations and potentially targetable biomarkers. In this study, we explored immunohistochemical and molecular genetic characteristics of 37 invasive apocrine carcinomas using immunohistochemistry (IHC), fluorescent in situ hybridization (FISH), multiplex ligation-dependent probe amplification (MLPA), and next-generation sequencing (NGS) assays. IHC revealed frequent E-cadherin expression (89%), moderate (16%) proliferation activity [Ki-67, phosphohistone H3], infrequent (~10%) expression of basal cell markers [CK5/6, CK14, p63, caveolin-1], loss of PTEN (83%), and overexpression of HER2 (32%), EGFR (41%), cyclin D1 (50%), and MUC-1 (88%). MLPA assay revealed gene copy gains of MYC, CCND1, ZNF703, CDH1, and TRAF4 in 50% or greater of the apocrine carcinomas, whereas gene copy losses frequently affected BRCA2 (75%), ADAM9 (54%), and BRCA1 (46%). HER2 gain, detected by MLPA in 38% of the cases, was in excellent concordance with HER2 results obtained by IHC/FISH (κ = 0.915, P
Idioma originalEnglish (US)
Páginas1350-1359
Número de páginas10
DOI
EstadoPublished - ene 1 2015
Eventoconference -
Duración: ene 1 2015 → …

Conference

Conferenceconference
Período1/1/15 → …

Huella dactilar

Multiplex Polymerase Chain Reaction
Immunohistochemistry
Pathology
Carcinoma
Fluorescence In Situ Hybridization
TNF Receptor-Associated Factor 4
Molecular Biology
Caveolin 1
Cyclin D1
Cadherins
Genes
Biomarkers

Citar esto

Vranic, S., Marchiò, C., Castellano, I., Botta, C., Scalzo, M. S., Bender, R. P., ... Sapino, A. (2015). Human Pathology. 1350-1359. Papel presentado en conference, . https://doi.org/10.1016/j.humpath.2015.05.017
Vranic, Semir ; Marchiò, Caterina ; Castellano, Isabella ; Botta, Cristina ; Scalzo, Maria Stella ; Bender, Ryan P. ; Payan-Gomez, Cesar ; Di Cantogno, Ludovica Verdun ; Gugliotta, Patrizia ; Tondat, Fabrizio ; Di Celle, Paola Francia ; Mariani, Sara ; Gatalica, Zoran ; Sapino, Anna. / Human Pathology. Papel presentado en conference, .10 p.
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Vranic, S, Marchiò, C, Castellano, I, Botta, C, Scalzo, MS, Bender, RP, Payan-Gomez, C, Di Cantogno, LV, Gugliotta, P, Tondat, F, Di Celle, PF, Mariani, S, Gatalica, Z & Sapino, A 2015, 'Human Pathology' Papel presentado en, 1/1/15, pp. 1350-1359. https://doi.org/10.1016/j.humpath.2015.05.017

Human Pathology. / Vranic, Semir; Marchiò, Caterina; Castellano, Isabella; Botta, Cristina; Scalzo, Maria Stella; Bender, Ryan P.; Payan-Gomez, Cesar; Di Cantogno, Ludovica Verdun; Gugliotta, Patrizia; Tondat, Fabrizio; Di Celle, Paola Francia; Mariani, Sara; Gatalica, Zoran; Sapino, Anna.

2015. 1350-1359 Papel presentado en conference, .

Resultado de la investigación: Tipos de Contribuciónes en ConferenciaPaper

TY - CONF

T1 - Human Pathology

AU - Vranic, Semir

AU - Marchiò, Caterina

AU - Castellano, Isabella

AU - Botta, Cristina

AU - Scalzo, Maria Stella

AU - Bender, Ryan P.

AU - Payan-Gomez, Cesar

AU - Di Cantogno, Ludovica Verdun

AU - Gugliotta, Patrizia

AU - Tondat, Fabrizio

AU - Di Celle, Paola Francia

AU - Mariani, Sara

AU - Gatalica, Zoran

AU - Sapino, Anna

PY - 2015/1/1

Y1 - 2015/1/1

N2 - © 2015 Elsevier Inc.Summary Despite the marked improvement in the understanding of molecular mechanisms and classification of apocrine carcinoma, little is known about its specific molecular genetic alterations and potentially targetable biomarkers. In this study, we explored immunohistochemical and molecular genetic characteristics of 37 invasive apocrine carcinomas using immunohistochemistry (IHC), fluorescent in situ hybridization (FISH), multiplex ligation-dependent probe amplification (MLPA), and next-generation sequencing (NGS) assays. IHC revealed frequent E-cadherin expression (89%), moderate (16%) proliferation activity [Ki-67, phosphohistone H3], infrequent (~10%) expression of basal cell markers [CK5/6, CK14, p63, caveolin-1], loss of PTEN (83%), and overexpression of HER2 (32%), EGFR (41%), cyclin D1 (50%), and MUC-1 (88%). MLPA assay revealed gene copy gains of MYC, CCND1, ZNF703, CDH1, and TRAF4 in 50% or greater of the apocrine carcinomas, whereas gene copy losses frequently affected BRCA2 (75%), ADAM9 (54%), and BRCA1 (46%). HER2 gain, detected by MLPA in 38% of the cases, was in excellent concordance with HER2 results obtained by IHC/FISH (κ = 0.915, P

AB - © 2015 Elsevier Inc.Summary Despite the marked improvement in the understanding of molecular mechanisms and classification of apocrine carcinoma, little is known about its specific molecular genetic alterations and potentially targetable biomarkers. In this study, we explored immunohistochemical and molecular genetic characteristics of 37 invasive apocrine carcinomas using immunohistochemistry (IHC), fluorescent in situ hybridization (FISH), multiplex ligation-dependent probe amplification (MLPA), and next-generation sequencing (NGS) assays. IHC revealed frequent E-cadherin expression (89%), moderate (16%) proliferation activity [Ki-67, phosphohistone H3], infrequent (~10%) expression of basal cell markers [CK5/6, CK14, p63, caveolin-1], loss of PTEN (83%), and overexpression of HER2 (32%), EGFR (41%), cyclin D1 (50%), and MUC-1 (88%). MLPA assay revealed gene copy gains of MYC, CCND1, ZNF703, CDH1, and TRAF4 in 50% or greater of the apocrine carcinomas, whereas gene copy losses frequently affected BRCA2 (75%), ADAM9 (54%), and BRCA1 (46%). HER2 gain, detected by MLPA in 38% of the cases, was in excellent concordance with HER2 results obtained by IHC/FISH (κ = 0.915, P

U2 - 10.1016/j.humpath.2015.05.017

DO - 10.1016/j.humpath.2015.05.017

M3 - Paper

SP - 1350

EP - 1359

ER -

Vranic S, Marchiò C, Castellano I, Botta C, Scalzo MS, Bender RP y otros. Human Pathology. 2015. Papel presentado en conference, . https://doi.org/10.1016/j.humpath.2015.05.017