Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples

Marina Munoz, Milena Camargo, Sara C. Soto-De Leon, Ricardo Sanchez, Diana Parra, Andrea C. Pineda, Otto Sussmann, Antonio Perez-Prados, Manuel E. Patarroyo, Manuel A. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

9 Citas (Scopus)

Resumen

Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance. © 2013 Munoz et al.
Idioma originalEnglish (US)
PublicaciónPLoS One
DOI
EstadoPublished - feb 13 2013

Huella dactilar

Papillomaviridae
Human immunodeficiency virus
Viruses
urine
HIV
Urine
Polymerase chain reaction
DNA
Screening
Human papillomavirus 16
sampling
Coinfection
Human papillomavirus 31
mixed infection
Human papillomavirus 11
Human papillomavirus 6
Human papillomavirus 18
Papillomavirus Infections
Infection
infection

Citar esto

Munoz, Marina ; Camargo, Milena ; Soto-De Leon, Sara C. ; Sanchez, Ricardo ; Parra, Diana ; Pineda, Andrea C. ; Sussmann, Otto ; Perez-Prados, Antonio ; Patarroyo, Manuel E. ; Patarroyo, Manuel A. / Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples. En: PLoS One. 2013.
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title = "Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples",
abstract = "Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6{\%} in cervical and 63.2{\%} in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7{\%} in cervical samples and 62.0{\%} in urine) followed by HPV-31(47.2{\%}) in cervical samples and HPV-58 (35.7{\%}) in urine samples. There was 55.4{\%} coinfection (infection by more than one type of HPV) in cervical samples and 40.2{\%} in urine samples. Abnormal Papanicolau smears were observed in 25.3{\%} of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance. {\circledC} 2013 Munoz et al.",
author = "Marina Munoz and Milena Camargo and {Soto-De Leon}, {Sara C.} and Ricardo Sanchez and Diana Parra and Pineda, {Andrea C.} and Otto Sussmann and Antonio Perez-Prados and Patarroyo, {Manuel E.} and Patarroyo, {Manuel A.}",
year = "2013",
month = "2",
day = "13",
doi = "10.1371/journal.pone.0056509",
language = "English (US)",
journal = "PLoS One",
issn = "1932-6203",
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Munoz, M, Camargo, M, Soto-De Leon, SC, Sanchez, R, Parra, D, Pineda, AC, Sussmann, O, Perez-Prados, A, Patarroyo, ME & Patarroyo, MA 2013, 'Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples', PLoS One. https://doi.org/10.1371/journal.pone.0056509

Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples. / Munoz, Marina; Camargo, Milena; Soto-De Leon, Sara C.; Sanchez, Ricardo; Parra, Diana; Pineda, Andrea C.; Sussmann, Otto; Perez-Prados, Antonio; Patarroyo, Manuel E.; Patarroyo, Manuel A.

En: PLoS One, 13.02.2013.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Human Papillomavirus Detection from Human Immunodeficiency Virus-Infected Colombian Women's Paired Urine and Cervical Samples

AU - Munoz, Marina

AU - Camargo, Milena

AU - Soto-De Leon, Sara C.

AU - Sanchez, Ricardo

AU - Parra, Diana

AU - Pineda, Andrea C.

AU - Sussmann, Otto

AU - Perez-Prados, Antonio

AU - Patarroyo, Manuel E.

AU - Patarroyo, Manuel A.

PY - 2013/2/13

Y1 - 2013/2/13

N2 - Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance. © 2013 Munoz et al.

AB - Infection, coinfection and type-specific human papillomavirus (HPV) distribution was evaluated in human immunodeficiency virus (HIV)-positive women from paired cervical and urine samples. Paired cervical and urine samples (n = 204) were taken from HIV-positive women for identifying HPV-DNA presence by using polymerase chain reaction (PCR) with three generic primer sets (GP5+/6+, MY09/11 and pU1M/2R). HPV-positive samples were typed for six high-risk HPV (HR-HPV) (HPV-16, -18, -31, -33, -45 and -58) and two low-risk (LR-HPV) (HPV-6/11) types. Agreement between paired sample results and diagnostic performance was evaluated. HPV infection prevalence was 70.6% in cervical and 63.2% in urine samples. HPV-16 was the most prevalent HPV type in both types of sample (66.7% in cervical samples and 62.0% in urine) followed by HPV-31(47.2%) in cervical samples and HPV-58 (35.7%) in urine samples. There was 55.4% coinfection (infection by more than one type of HPV) in cervical samples and 40.2% in urine samples. Abnormal Papanicolau smears were observed in 25.3% of the women, presenting significant association with HPV-DNA being identified in urine samples. There was poor agreement of cervical and urine sample results in generic and type-specific detection of HPV. Urine samples provided the best diagnosis when taking cytological findings as reference. In conclusion including urine samples could be a good strategy for ensuring adherence to screening programs aimed at reducing the impact of cervical cancer, since this sample is easy to obtain and showed good diagnostic performance. © 2013 Munoz et al.

U2 - 10.1371/journal.pone.0056509

DO - 10.1371/journal.pone.0056509

M3 - Article

JO - PLoS One

JF - PLoS One

SN - 1932-6203

ER -