Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Glaucia Diniz Alessio; Fernanda Fortes de Araújo; Jéssica Spínola Silva; Policarpo Ademar Sales Júnior; Matheus de Souza Gomes; Laurence Rodrigues do Amaral; Juan David Ramírez; Carolina Flórez; Andréa Teixeira-Carvalho; Melina de Barros Pinheiro; Marta de Lana; Olindo Assis Martins-Filho

doi:10.1038/s41598-020-69921-z

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Título traducido de la contribución: Chagas-Flow humano ATE-IgG1 para el serodiagnóstico universal avanzado y específico de Trypanosoma cruzi

Glaucia Diniz Alessio
, Fernanda Fortes de Araújo
, Jéssica Spínola Silva
, Policarpo Ademar Sales Júnior
, Matheus de Souza Gomes
, Laurence Rodrigues do Amaral
, Juan David Ramírez
, Carolina Flórez
, Andréa Teixeira-Carvalho
, Melina de Barros Pinheiro
, Marta de Lana
, Olindo Assis Martins-Filho

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

8 Citas (Scopus)

Resumen

Los métodos moleculares y serológicos disponibles para el diagnóstico específico de Trypanosoma cruzi en la enfermedad de Chagas crónica presentan limitaciones. El estudio evaluó el rendimiento de Human Chagas-Flow ATE-IgG1 para el diagnóstico universal y específico de la enfermedad de Chagas. Un total de 102 sueros de pacientes con enfermedad de Chagas (CH) infectados crónicamente con DTUs TcI, TcVI o TcII fueron analizados para determinar la reactividad de IgG1 a los antígenos amastigote/(A), tripomastigote/(T) y epimastigote/(E) a lo largo de la curva de titulación (1:250-1:32.000). Los resultados demostraron que "AI 250/40%", "EVI 250/30%", "AII 250/40%", "TII 250/40%" y "EII 250/30%" tienen una precisión extraordinaria (100%) para segregar los CH de los controles no infectados. Los atributos "TI 4.000/50%", "EI 2.000/50%", "AVI 8.000/60%" y "TVI 4.000/50%" fueron seleccionados para el serotipado específico de la enfermedad de Chagas. El uso aislado de "EI 2.000/50%" proporcionó la mayor co-positividad para los pacientes con TcI (91%). Los algoritmos combinados de árboles de decisión que utilizan los conjuntos predefinidos de atributos mostraron una precisión total sobresaliente (92% y 97%) para discriminar los prototipos "TcI vs TcVI vs TcII" y "TcI vs TcII", respectivamente. El elevado rendimiento de Human Chagas-Flow ATE-IgG1 califica su uso para el diagnóstico universal y específico de TcI/TcVI/TcII de la enfermedad de Chagas. Estos resultados apoyan la aplicación de este método en las encuestas epidemiológicas, la monitorización post-terapéutica y el seguimiento de los resultados clínicos de la enfermedad de Chagas.

Título traducido de la contribución	Chagas-Flow humano ATE-IgG1 para el serodiagnóstico universal avanzado y específico de Trypanosoma cruzi
Idioma original	Inglés estadounidense
Número de artículo	13296
Páginas (desde-hasta)	1-14
Número de páginas	14
Publicación	Scientific Reports
Volumen	10
N.º	1
DOI	https://doi.org/10.1038/s41598-020-69921-z
Estado	Publicada - ago. 6 2020

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Alessio, G. D., de Araújo, F. F., Silva, J. S., Júnior, P. A. S., de Souza Gomes, M., do Amaral, L. R., Ramírez, J. D., Flórez, C., Teixeira-Carvalho, A., de Barros Pinheiro, M., de Lana, M., & Martins-Filho, O. A. (2020). Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Scientific Reports, 10(1), 1-14. Artículo 13296. https://doi.org/10.1038/s41598-020-69921-z

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title = "Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease",

abstract = "The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40\%”, “EVI 250/30\%”, “AII 250/40\%”, “TII 250/40\%” and “EII 250/30\%” have outstanding accuracy (100\%) to segregate CH from non-infected controls. The attributes “TI 4,000/50\%”, “EI 2,000/50\%”, “AVI 8,000/60\%” and “TVI 4,000/50\%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50\%” provided the highest co-positivity for TcI patients (91\%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92\% and 97\%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.",

author = "Alessio, \{Glaucia Diniz\} and \{de Ara{\'u}jo\}, \{Fernanda Fortes\} and Silva, \{J{\'e}ssica Sp{\'i}nola\} and J{\'u}nior, \{Policarpo Ademar Sales\} and \{de Souza Gomes\}, Matheus and \{do Amaral\}, \{Laurence Rodrigues\} and Ram{\'i}rez, \{Juan David\} and Carolina Fl{\'o}rez and Andr{\'e}a Teixeira-Carvalho and \{de Barros Pinheiro\}, Melina and \{de Lana\}, Marta and Martins-Filho, \{Olindo Assis\}",

year = "2020",

month = aug,

day = "6",

doi = "10.1038/s41598-020-69921-z",

language = "English (US)",

volume = "10",

pages = "1--14",

journal = "Scientific Reports",

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Alessio, GD, de Araújo, FF, Silva, JS, Júnior, PAS, de Souza Gomes, M, do Amaral, LR, Ramírez, JD, Flórez, C, Teixeira-Carvalho, A, de Barros Pinheiro, M, de Lana, M & Martins-Filho, OA 2020, 'Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease', Scientific Reports, vol. 10, n.º 1, 13296, pp. 1-14. https://doi.org/10.1038/s41598-020-69921-z

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. / Alessio, Glaucia Diniz; de Araújo, Fernanda Fortes; Silva, Jéssica Spínola et al.
En: Scientific Reports, Vol. 10, N.º 1, 13296, 06.08.2020, p. 1-14.

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

TY - JOUR

T1 - Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

AU - Alessio, Glaucia Diniz

AU - de Araújo, Fernanda Fortes

AU - Silva, Jéssica Spínola

AU - Júnior, Policarpo Ademar Sales

AU - de Souza Gomes, Matheus

AU - do Amaral, Laurence Rodrigues

AU - Ramírez, Juan David

AU - Flórez, Carolina

AU - Teixeira-Carvalho, Andréa

AU - de Barros Pinheiro, Melina

AU - de Lana, Marta

AU - Martins-Filho, Olindo Assis

PY - 2020/8/6

Y1 - 2020/8/6

N2 - The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.

AB - The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.

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UR - https://www.scopus.com/pages/publications/85089157619#tab=citedBy

U2 - 10.1038/s41598-020-69921-z

DO - 10.1038/s41598-020-69921-z

M3 - Research Article

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AN - SCOPUS:85089157619

SN - 2045-2322

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JO - Scientific Reports

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ER -

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

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