Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Glaucia Diniz Alessio; Fernanda Fortes de Araújo; Jéssica Spínola Silva; Policarpo Ademar Sales Júnior; Matheus de Souza Gomes; Laurence Rodrigues do Amaral; Juan David Ramírez; Carolina Flórez; Andréa Teixeira-Carvalho; Melina de Barros Pinheiro; Marta de Lana; Olindo Assis Martins-Filho

doi:10.1038/s41598-020-69921-z

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

Título traducido de la contribución: Chagas-Flow humano ATE-IgG1 para el serodiagnóstico universal avanzado y específico de Trypanosoma cruzi

Glaucia Diniz Alessio, Fernanda Fortes de Araújo, Jéssica Spínola Silva, Policarpo Ademar Sales Júnior, Matheus de Souza Gomes, Laurence Rodrigues do Amaral, Juan David Ramírez, Carolina Flórez, Andréa Teixeira-Carvalho, Melina de Barros Pinheiro, Marta de Lana, Olindo Assis Martins-Filho

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Resumen

Los métodos moleculares y serológicos disponibles para el diagnóstico específico de Trypanosoma cruzi en la enfermedad de Chagas crónica presentan limitaciones. El estudio evaluó el rendimiento de Human Chagas-Flow ATE-IgG1 para el diagnóstico universal y específico de la enfermedad de Chagas. Un total de 102 sueros de pacientes con enfermedad de Chagas (CH) infectados crónicamente con DTUs TcI, TcVI o TcII fueron analizados para determinar la reactividad de IgG1 a los antígenos amastigote/(A), tripomastigote/(T) y epimastigote/(E) a lo largo de la curva de titulación (1:250-1:32.000). Los resultados demostraron que "AI 250/40%", "EVI 250/30%", "AII 250/40%", "TII 250/40%" y "EII 250/30%" tienen una precisión extraordinaria (100%) para segregar los CH de los controles no infectados. Los atributos "TI 4.000/50%", "EI 2.000/50%", "AVI 8.000/60%" y "TVI 4.000/50%" fueron seleccionados para el serotipado específico de la enfermedad de Chagas. El uso aislado de "EI 2.000/50%" proporcionó la mayor co-positividad para los pacientes con TcI (91%). Los algoritmos combinados de árboles de decisión que utilizan los conjuntos predefinidos de atributos mostraron una precisión total sobresaliente (92% y 97%) para discriminar los prototipos "TcI vs TcVI vs TcII" y "TcI vs TcII", respectivamente. El elevado rendimiento de Human Chagas-Flow ATE-IgG1 califica su uso para el diagnóstico universal y específico de TcI/TcVI/TcII de la enfermedad de Chagas. Estos resultados apoyan la aplicación de este método en las encuestas epidemiológicas, la monitorización post-terapéutica y el seguimiento de los resultados clínicos de la enfermedad de Chagas.

Título traducido de la contribución	Chagas-Flow humano ATE-IgG1 para el serodiagnóstico universal avanzado y específico de Trypanosoma cruzi
Idioma original	Inglés estadounidense
Número de artículo	13296
Páginas (desde-hasta)	1-14
Número de páginas	14
Publicación	Scientific Reports
Volumen	10
N.º	1
DOI	https://doi.org/10.1038/s41598-020-69921-z
Estado	Publicada - ago. 6 2020

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Alessio, G. D., de Araújo, F. F., Silva, J. S., Júnior, P. A. S., de Souza Gomes, M., do Amaral, L. R., Ramírez, J. D., Flórez, C., Teixeira-Carvalho, A., de Barros Pinheiro, M., de Lana, M., & Martins-Filho, O. A. (2020). Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. Scientific Reports, 10(1), 1-14. Artículo 13296. https://doi.org/10.1038/s41598-020-69921-z

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title = "Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease",

abstract = "The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.",

author = "Alessio, {Glaucia Diniz} and {de Ara{\'u}jo}, {Fernanda Fortes} and Silva, {J{\'e}ssica Sp{\'i}nola} and J{\'u}nior, {Policarpo Ademar Sales} and {de Souza Gomes}, Matheus and {do Amaral}, {Laurence Rodrigues} and Ram{\'i}rez, {Juan David} and Carolina Fl{\'o}rez and Andr{\'e}a Teixeira-Carvalho and {de Barros Pinheiro}, Melina and {de Lana}, Marta and Martins-Filho, {Olindo Assis}",

year = "2020",

month = aug,

day = "6",

doi = "10.1038/s41598-020-69921-z",

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Alessio, GD, de Araújo, FF, Silva, JS, Júnior, PAS, de Souza Gomes, M, do Amaral, LR, Ramírez, JD, Flórez, C, Teixeira-Carvalho, A, de Barros Pinheiro, M, de Lana, M & Martins-Filho, OA 2020, 'Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease', Scientific Reports, vol. 10, n.º 1, 13296, pp. 1-14. https://doi.org/10.1038/s41598-020-69921-z

Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease. / Alessio, Glaucia Diniz; de Araújo, Fernanda Fortes; Silva, Jéssica Spínola et al.
En: Scientific Reports, Vol. 10, N.º 1, 13296, 06.08.2020, p. 1-14.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

AU - Alessio, Glaucia Diniz

AU - de Araújo, Fernanda Fortes

AU - Silva, Jéssica Spínola

AU - Júnior, Policarpo Ademar Sales

AU - de Souza Gomes, Matheus

AU - do Amaral, Laurence Rodrigues

AU - Ramírez, Juan David

AU - Flórez, Carolina

AU - Teixeira-Carvalho, Andréa

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AU - de Lana, Marta

AU - Martins-Filho, Olindo Assis

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AB - The molecular and serological methods available for Discrete Typing Units (DTU)-specific diagnosis of Trypanosoma cruzi in chronic Chagas disease present limitations. The study evaluated the performance of Human Chagas-Flow ATE-IgG1 for universal and DTU-specific diagnosis of Chagas disease. A total of 102 sera from Chagas disease patients (CH) chronically infected with TcI, TcVI or TcII DTUs were tested for IgG1 reactivity to amastigote/(A), trypomastigote/(T) and epimastigote/(E) antigens along the titration curve (1:250–1:32,000). The results demonstrated that “AI 250/40%”, “EVI 250/30%”, “AII 250/40%”, “TII 250/40%” and “EII 250/30%” have outstanding accuracy (100%) to segregate CH from non-infected controls. The attributes “TI 4,000/50%”, “EI 2,000/50%”, “AVI 8,000/60%” and “TVI 4,000/50%” were selected for DTU-specific serotyping of Chagas disease. The isolated use of “EI 2,000/50%” provided the highest co-positivity for TcI patients (91%). The combined decision tree algorithms using the pre-defined sets of attributes showed outstanding full accuracy (92% and 97%) to discriminate “TcI vs TcVI vs TcII” and “TcI vs TcII” prototypes, respectively. The elevated performance of Human Chagas-Flow ATE-IgG1 qualifies its use for universal and TcI/TcVI/TcII-specific diagnosis of Chagas disease. These findings further support the application of this method in epidemiological surveys, post-therapeutic monitoring and clinical outcome follow-ups for Chagas disease.

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Human Chagas-Flow ATE-IgG1 for advanced universal and Trypanosoma cruzi Discrete Typing Units-specific serodiagnosis of Chagas disease

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