Highlights of infectious agents in tissue

Guillermo Antonio Jimenez Tobon; Alejandro Vélez

doi:10.1016/j.pathol.2021.10.004

Highlights of infectious agents in tissue

Título traducido de la contribución: Puntos importantes en agentes infecciosos en tejido

Guillermo Antonio Jimenez Tobon
, Alejandro Vélez

Producción científica: Contribución a revista › Artículo de Investigación › revisión exhaustiva

6 Citas (Scopus)

Resumen

La evolución del diagnóstico de las enfermedades infecciosas comenzó con la observación de las características morfológicas de organismos como los áscaris y los tricocéfalos, seguida del uso del microscopio y las tinciones de hematoxilina y eosina, que permitieron reconocer características microscópicas indetectables a simple vista, como los cambios citopáticos víricos del herpes y la presencia de hongos. También se observaron patrones de inflamación granulomatosa aguda y crónica, que no eran específicos de la etiología exacta de la enfermedad, lo que llevó a la introducción de tinciones especiales de metenamina para hongos y de Ziehl-Neelsen para hongos y micobacterias. Posteriormente, se introdujo el uso de la inmunohistoquímica, que reconocía el uso de anticuerpos para clasificar los microorganismos y detectar casos difíciles de interpretar o en medio de procesos inflamatorios graves. En la actualidad, el uso de la biología molecular ha permitido alcanzar diagnósticos que hubieran sido muy difíciles de obtener mediante los métodos tradicionales; estas técnicas muestran características específicas clave y facilitan el diagnóstico de diversas patologías infecciosas. Estas nuevas técnicas se basan en la detección de antígenos y ácidos nucleicos de microorganismos, lo que supone un importante avance en el diagnóstico de las enfermedades infecciosas.

Título traducido de la contribución	Puntos importantes en agentes infecciosos en tejido
Idioma original	Inglés estadounidense
Páginas (desde-hasta)	217-224
Número de páginas	8
Publicación	Pathology
Volumen	54
N.º	2
DOI	https://doi.org/10.1016/j.pathol.2021.10.004
Estado	Publicada - abr. 1 2022

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Este resultado contribuye a los siguientes Objetivos de Desarrollo Sostenible

ODS 3: Salud y bienestar

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Patología y medicina forense

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10.1016/j.pathol.2021.10.004

https://www.sciencedirect.com/science/article/pii/S0031302521005432?via%3DihubLicencia: Otros

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title = "Highlights of infectious agents in tissue",

abstract = "The evolution of the diagnosis of infectious diseases began with the observation of the morphological characteristics of organisms such as ascaris and whipworms, followed by the use of the microscope and haematoxylin and eosin stains, which allowed recognition of microscopic characteristics undetectable with the naked eye, such as the viral cytopathic changes of herpes and the presence of fungi. Patterns of acute and chronic granulomatous inflammation were also observed; these were not specific to the exact aetiology of the disease, which led to the introduction of special methenamine stains for fungi and Ziehl–Neelsen for fungi and mycobacteria. Later, the use of immunohistochemistry was introduced, which acknowledged the use of antibodies to classify microorganisms and detect cases that were either difficult to interpret or in the midst of severe inflammatory processes. Currently, the use of molecular biology has made it possible to reach diagnoses that would have been very difficult to obtain through traditional methods; these techniques show key specific characteristics and facilitate the diagnosis of various infectious pathologies. These new techniques are based on the detection of antigens and nucleic acids of microorganisms, an important advance in the diagnosis of infectious diseases.",

author = "\{Jimenez Tobon\}, \{Guillermo Antonio\} and Alejandro V{\'e}lez",

year = "2022",

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doi = "10.1016/j.pathol.2021.10.004",

language = "English (US)",

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pages = "217--224",

journal = "Pathology",

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T1 - Highlights of infectious agents in tissue

AU - Jimenez Tobon, Guillermo Antonio

AU - Vélez, Alejandro

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N2 - The evolution of the diagnosis of infectious diseases began with the observation of the morphological characteristics of organisms such as ascaris and whipworms, followed by the use of the microscope and haematoxylin and eosin stains, which allowed recognition of microscopic characteristics undetectable with the naked eye, such as the viral cytopathic changes of herpes and the presence of fungi. Patterns of acute and chronic granulomatous inflammation were also observed; these were not specific to the exact aetiology of the disease, which led to the introduction of special methenamine stains for fungi and Ziehl–Neelsen for fungi and mycobacteria. Later, the use of immunohistochemistry was introduced, which acknowledged the use of antibodies to classify microorganisms and detect cases that were either difficult to interpret or in the midst of severe inflammatory processes. Currently, the use of molecular biology has made it possible to reach diagnoses that would have been very difficult to obtain through traditional methods; these techniques show key specific characteristics and facilitate the diagnosis of various infectious pathologies. These new techniques are based on the detection of antigens and nucleic acids of microorganisms, an important advance in the diagnosis of infectious diseases.

AB - The evolution of the diagnosis of infectious diseases began with the observation of the morphological characteristics of organisms such as ascaris and whipworms, followed by the use of the microscope and haematoxylin and eosin stains, which allowed recognition of microscopic characteristics undetectable with the naked eye, such as the viral cytopathic changes of herpes and the presence of fungi. Patterns of acute and chronic granulomatous inflammation were also observed; these were not specific to the exact aetiology of the disease, which led to the introduction of special methenamine stains for fungi and Ziehl–Neelsen for fungi and mycobacteria. Later, the use of immunohistochemistry was introduced, which acknowledged the use of antibodies to classify microorganisms and detect cases that were either difficult to interpret or in the midst of severe inflammatory processes. Currently, the use of molecular biology has made it possible to reach diagnoses that would have been very difficult to obtain through traditional methods; these techniques show key specific characteristics and facilitate the diagnosis of various infectious pathologies. These new techniques are based on the detection of antigens and nucleic acids of microorganisms, an important advance in the diagnosis of infectious diseases.

U2 - 10.1016/j.pathol.2021.10.004

DO - 10.1016/j.pathol.2021.10.004

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ER -

Highlights of infectious agents in tissue

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