High plasmodium malariae prevalence in an endemic area of the colombian amazon region

Paola Andrea Camargo-Ayala, Juan Ricardo Cubides, Carlos Hernando Niño, Milena Camargo, Carlos Arturo Rodríguez-Celis, Teódulo Quiñones, Lizeth Sánchez-Suárez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

11 Citas (Scopus)

Resumen

© 2016 Camargo-Ayala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malaria is a worldwide public health problem; parasites from the genus Plasmodium are the aetiological agent for this disease. The parasites are mostly diagnosed by conventional microscopy-based techniques; however, their limitations have led to under-registering the reported prevalence of Plasmodium species. This study has thus been aimed at evaluating the infection and coinfection prevalence of 3 species of Plasmodium spp., in an area of the Colombian Amazon region. Blood samples were taken from 671 symptomatic patients by skin puncture; a nested PCR amplifying the 18S ssRNA region was used on all samples to determine the presence of P. vivax, P. malariae and P. falciparum. Statistical analysis determined infection and coinfection frequency; the association between infection and different factors was established. The results showed that P. vivax was the species having the greatest frequency in the study population (61.4%), followed by P. malariae (43.8%) and P. falciparum (11.8%). The study revealed that 35.8% of the population had coinfection, the P. vivax/P. malariae combination occurring most frequently (28.3%); factors such as age, geographical origin and clinical manifestations were found to be associated with triple-infection. The prevalence reported in this study differed from previous studies in Colombia; the results suggest that diagnosis using conventional techniques could be giving rise to underestimating some Plasmodium spp. species having high circulation rates in Colombia (particularly in the Colombian Amazon region). The present study's results revealed a high prevalence of P. malariae and mixed infections in the population being studied. The results provide relevant information which should facilitate updating the epidemiological panorama and species' distribution so as to include control, prevention and follow-up measures.
Idioma originalEnglish (US)
PublicaciónPLoS One
DOI
EstadoPublished - jul 1 2016

Huella dactilar

Plasmodium malariae
Plasmodium
Amazonia
Coinfection
mixed infection
Vivax Malaria
Colombia
Public health
Medical problems
Infection
infection
Statistical methods
Skin
Microscopic examination
Parasites
Blood
Population
parasites
Falciparum Malaria
etiological agents

Citar esto

Camargo-Ayala, P. A., Cubides, J. R., Niño, C. H., Camargo, M., Rodríguez-Celis, C. A., Quiñones, T., ... Patarroyo, M. A. (2016). High plasmodium malariae prevalence in an endemic area of the colombian amazon region. PLoS One. https://doi.org/10.1371/journal.pone.0159968
Camargo-Ayala, Paola Andrea ; Cubides, Juan Ricardo ; Niño, Carlos Hernando ; Camargo, Milena ; Rodríguez-Celis, Carlos Arturo ; Quiñones, Teódulo ; Sánchez-Suárez, Lizeth ; Patarroyo, Manuel Elkin ; Patarroyo, Manuel Alfonso. / High plasmodium malariae prevalence in an endemic area of the colombian amazon region. En: PLoS One. 2016.
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abstract = "{\circledC} 2016 Camargo-Ayala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malaria is a worldwide public health problem; parasites from the genus Plasmodium are the aetiological agent for this disease. The parasites are mostly diagnosed by conventional microscopy-based techniques; however, their limitations have led to under-registering the reported prevalence of Plasmodium species. This study has thus been aimed at evaluating the infection and coinfection prevalence of 3 species of Plasmodium spp., in an area of the Colombian Amazon region. Blood samples were taken from 671 symptomatic patients by skin puncture; a nested PCR amplifying the 18S ssRNA region was used on all samples to determine the presence of P. vivax, P. malariae and P. falciparum. Statistical analysis determined infection and coinfection frequency; the association between infection and different factors was established. The results showed that P. vivax was the species having the greatest frequency in the study population (61.4{\%}), followed by P. malariae (43.8{\%}) and P. falciparum (11.8{\%}). The study revealed that 35.8{\%} of the population had coinfection, the P. vivax/P. malariae combination occurring most frequently (28.3{\%}); factors such as age, geographical origin and clinical manifestations were found to be associated with triple-infection. The prevalence reported in this study differed from previous studies in Colombia; the results suggest that diagnosis using conventional techniques could be giving rise to underestimating some Plasmodium spp. species having high circulation rates in Colombia (particularly in the Colombian Amazon region). The present study's results revealed a high prevalence of P. malariae and mixed infections in the population being studied. The results provide relevant information which should facilitate updating the epidemiological panorama and species' distribution so as to include control, prevention and follow-up measures.",
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Camargo-Ayala, PA, Cubides, JR, Niño, CH, Camargo, M, Rodríguez-Celis, CA, Quiñones, T, Sánchez-Suárez, L, Patarroyo, ME & Patarroyo, MA 2016, 'High plasmodium malariae prevalence in an endemic area of the colombian amazon region', PLoS One. https://doi.org/10.1371/journal.pone.0159968

High plasmodium malariae prevalence in an endemic area of the colombian amazon region. / Camargo-Ayala, Paola Andrea; Cubides, Juan Ricardo; Niño, Carlos Hernando; Camargo, Milena; Rodríguez-Celis, Carlos Arturo; Quiñones, Teódulo; Sánchez-Suárez, Lizeth; Patarroyo, Manuel Elkin; Patarroyo, Manuel Alfonso.

En: PLoS One, 01.07.2016.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - High plasmodium malariae prevalence in an endemic area of the colombian amazon region

AU - Camargo-Ayala, Paola Andrea

AU - Cubides, Juan Ricardo

AU - Niño, Carlos Hernando

AU - Camargo, Milena

AU - Rodríguez-Celis, Carlos Arturo

AU - Quiñones, Teódulo

AU - Sánchez-Suárez, Lizeth

AU - Patarroyo, Manuel Elkin

AU - Patarroyo, Manuel Alfonso

PY - 2016/7/1

Y1 - 2016/7/1

N2 - © 2016 Camargo-Ayala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malaria is a worldwide public health problem; parasites from the genus Plasmodium are the aetiological agent for this disease. The parasites are mostly diagnosed by conventional microscopy-based techniques; however, their limitations have led to under-registering the reported prevalence of Plasmodium species. This study has thus been aimed at evaluating the infection and coinfection prevalence of 3 species of Plasmodium spp., in an area of the Colombian Amazon region. Blood samples were taken from 671 symptomatic patients by skin puncture; a nested PCR amplifying the 18S ssRNA region was used on all samples to determine the presence of P. vivax, P. malariae and P. falciparum. Statistical analysis determined infection and coinfection frequency; the association between infection and different factors was established. The results showed that P. vivax was the species having the greatest frequency in the study population (61.4%), followed by P. malariae (43.8%) and P. falciparum (11.8%). The study revealed that 35.8% of the population had coinfection, the P. vivax/P. malariae combination occurring most frequently (28.3%); factors such as age, geographical origin and clinical manifestations were found to be associated with triple-infection. The prevalence reported in this study differed from previous studies in Colombia; the results suggest that diagnosis using conventional techniques could be giving rise to underestimating some Plasmodium spp. species having high circulation rates in Colombia (particularly in the Colombian Amazon region). The present study's results revealed a high prevalence of P. malariae and mixed infections in the population being studied. The results provide relevant information which should facilitate updating the epidemiological panorama and species' distribution so as to include control, prevention and follow-up measures.

AB - © 2016 Camargo-Ayala et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Malaria is a worldwide public health problem; parasites from the genus Plasmodium are the aetiological agent for this disease. The parasites are mostly diagnosed by conventional microscopy-based techniques; however, their limitations have led to under-registering the reported prevalence of Plasmodium species. This study has thus been aimed at evaluating the infection and coinfection prevalence of 3 species of Plasmodium spp., in an area of the Colombian Amazon region. Blood samples were taken from 671 symptomatic patients by skin puncture; a nested PCR amplifying the 18S ssRNA region was used on all samples to determine the presence of P. vivax, P. malariae and P. falciparum. Statistical analysis determined infection and coinfection frequency; the association between infection and different factors was established. The results showed that P. vivax was the species having the greatest frequency in the study population (61.4%), followed by P. malariae (43.8%) and P. falciparum (11.8%). The study revealed that 35.8% of the population had coinfection, the P. vivax/P. malariae combination occurring most frequently (28.3%); factors such as age, geographical origin and clinical manifestations were found to be associated with triple-infection. The prevalence reported in this study differed from previous studies in Colombia; the results suggest that diagnosis using conventional techniques could be giving rise to underestimating some Plasmodium spp. species having high circulation rates in Colombia (particularly in the Colombian Amazon region). The present study's results revealed a high prevalence of P. malariae and mixed infections in the population being studied. The results provide relevant information which should facilitate updating the epidemiological panorama and species' distribution so as to include control, prevention and follow-up measures.

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Camargo-Ayala PA, Cubides JR, Niño CH, Camargo M, Rodríguez-Celis CA, Quiñones T y otros. High plasmodium malariae prevalence in an endemic area of the colombian amazon region. PLoS One. 2016 jul 1. https://doi.org/10.1371/journal.pone.0159968