Plasmodium vivaxmerozoite surface protein 4 (PvMSP4) has been considered to be a promising malarial vaccine candidate. The antigenicdiversity displayed by parasite populations is one of the main factors limiting the efficacy of asexual-stage anti-malarial vaccine candidates.The present study is the first characterising PvMSP4 polymorphism.P. vivaxisolates were collected from endemic areas in Colombia anddiversity and selection pattern studies were carried out. Overall conservation in this protein was remarkable. Changes were only found inexons I and II, the former only having single nucleotide polymorphisms (SNPs) whilst the latter showed variations in tandem repeat numbercaused by exon II slippage. The remaining regions (EGF-like domain, GPI anchor and intron) were completely conserved. Selection andneutrality tests carried out over variant exons indicated negative selective forces acting on them. No evidence of intragenic recombination wasfound. The strong conservation displayed in this molecule by isolates from geographically different regions (Colombia, Salvador andThailand) stresses its potential importance as a candidate for a vaccine againstP. vivaxmalaria.#2004 Elsevier B.V. All rights reserved.
All Science Journal Classification (ASJC) codes
- Ecología, evolución, comportamiento y sistemática
- Biología molecular
- Microbiología (médica)
- Enfermedades infecciosas
Martinez, P., Suarez, C. F., Gomez, A., Cardenas, P. P., Guerrero, J. E., & Patarroyo, M. A. (2005). High level of conservation in Plasmodium vivax merozoite surface protein 4 (PvMSP4). Infection, Genetics and Evolution, 5(4), 354-361. https://doi.org/10.1016/j.meegid.2004.12.001