Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein

Manuel A. Patarroyo, David F. Plaza, Marisol Ocampo, Hernando Curtidor, Martha Forero, Luis E. Rodriguez, Manuel E. Patarroyo

Resultado de la investigación: Contribución a RevistaArtículo

10 Citas (Scopus)

Resumen

Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.
Idioma originalEnglish (US)
Páginas (desde-hasta)935-940
Número de páginas6
PublicaciónBiochemical and Biophysical Research Communications
DOI
EstadoPublished - ago 8 2008

Huella dactilar

Mycobacterium tuberculosis
Membrane Proteins
Tuberculosis Vaccines
Peptides
Genes
Bacilli
Microscopic examination
Proteins
Molecular weight
Cells
U937 Cells
Immunoelectron Microscopy
Mycobacterium bovis
Antibodies
Bacillus
Monocytes
Molecular Weight
Rabbits
Cell Line
Polymerase Chain Reaction

Citar esto

Patarroyo, Manuel A. ; Plaza, David F. ; Ocampo, Marisol ; Curtidor, Hernando ; Forero, Martha ; Rodriguez, Luis E. ; Patarroyo, Manuel E. / Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein. En: Biochemical and Biophysical Research Communications. 2008 ; pp. 935-940.
@article{02dca7c5a3244851bee5e559921ed703,
title = "Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein",
abstract = "Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. {\circledC} 2008 Elsevier Inc. All rights reserved.",
author = "Patarroyo, {Manuel A.} and Plaza, {David F.} and Marisol Ocampo and Hernando Curtidor and Martha Forero and Rodriguez, {Luis E.} and Patarroyo, {Manuel E.}",
year = "2008",
month = "8",
day = "8",
doi = "10.1016/j.bbrc.2008.05.157",
language = "English (US)",
pages = "935--940",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",

}

Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein. / Patarroyo, Manuel A.; Plaza, David F.; Ocampo, Marisol; Curtidor, Hernando; Forero, Martha; Rodriguez, Luis E.; Patarroyo, Manuel E.

En: Biochemical and Biophysical Research Communications, 08.08.2008, p. 935-940.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Functional characterization of Mycobacterium tuberculosis Rv2969c membrane protein

AU - Patarroyo, Manuel A.

AU - Plaza, David F.

AU - Ocampo, Marisol

AU - Curtidor, Hernando

AU - Forero, Martha

AU - Rodriguez, Luis E.

AU - Patarroyo, Manuel E.

PY - 2008/8/8

Y1 - 2008/8/8

N2 - Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.

AB - Identifying Mycobacterium tuberculosis membrane proteins involved in binding to and invasion of host cells is important in designing subunit-based anti-tuberculosis vaccines. The Rv2969c gene sequence was identified by PCR in M. tuberculosis complex strains, being transcribed in M. tuberculosis H37Rv, M. tuberculosis H37Ra, and M. bovis BCG. Rabbits immunized with synthetic peptides from highly specific conserved regions of this protein produced antibodies recognizing 27 and 29 kDa bands in M. tuberculosis lysate, which is consistent with the molecular weight of the Rv2969c gene product in M. tuberculosis H37Rv. Immunoelectron microscopy revealed the protein was localized on the bacillus surface. Four and three specific high activity binding peptides (HABPs) to the A549 alveolar epithelial and U937 monocyte cell lines were found, respectively. Two of the HABPs found inhibited M. tuberculosis invasion of A549 cells, suggesting that these peptides might be good candidates to be included in a multiepitopic, subunit-based anti-tuberculosis vaccine. © 2008 Elsevier Inc. All rights reserved.

U2 - 10.1016/j.bbrc.2008.05.157

DO - 10.1016/j.bbrc.2008.05.157

M3 - Article

SP - 935

EP - 940

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -