Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells

Lilian Chuaire-Noack, Pedro Monterrey-Gutiérrez, César E. Payán-Gómez

Resultado de la investigación: Contribución a RevistaArtículo

Resumen

GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.
Idioma originalEnglish (US)
Páginas (desde-hasta)219-228
Número de páginas10
PublicaciónRevista Ciencias de la Salud
EstadoPublished - dic 1 2011

Huella dactilar

Epithelial Cells
Telomere
Epithelium
Telomere Homeostasis
Telomere Shortening
Gene Expression
Primary Cell Culture
Cell Line
Polymerase Chain Reaction
DNA
In Vitro Techniques
Proteins

Citar esto

@article{e23a921e3ec34bcc93dbfd69110d6929,
title = "Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells",
abstract = "GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.",
author = "Lilian Chuaire-Noack and Pedro Monterrey-Guti{\'e}rrez and Pay{\'a}n-G{\'o}mez, {C{\'e}sar E.}",
year = "2011",
month = "12",
day = "1",
language = "English (US)",
pages = "219--228",
journal = "Revista Ciencias de la Salud",
issn = "1692-7273",
publisher = "Universidad del Rosario",

}

Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells. / Chuaire-Noack, Lilian; Monterrey-Gutiérrez, Pedro; Payán-Gómez, César E.

En: Revista Ciencias de la Salud, 01.12.2011, p. 219-228.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Expression of TRF2 and GAPDH in the aging of the in vitro ovarian surface epithelial cells

AU - Chuaire-Noack, Lilian

AU - Monterrey-Gutiérrez, Pedro

AU - Payán-Gómez, César E.

PY - 2011/12/1

Y1 - 2011/12/1

N2 - GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.

AB - GAPDH can bind single-strand telomere DNA both in vitro and in vivo. Thus, it was hypothesised that GAPDH has an important role in protecting the telomeres, role that could be shared with TRF2, a well-known telomeric protein involved in a myriad of functions related to telomere homeostasis. Objective: The aim of this study was to determine if there was a correlation between the expression of these genes in the in vitro ovarian surface epithelium. Materials and methods: The relative expression of each gene was established by qRT-PCR in primary cell cultures of the ovarian surface epithelium from 22 healthy mestizo Colombian donors. Results: The Kendall and Spearman non-parametric tests established a significant correlation between the levels of expression in subsequent passages of the cell line, in an age-independent way. Conclusion: Our findings suggest a synergistic effect between TRF2 and GAPDH that could counter telomere shortening in vitro.

M3 - Article

SP - 219

EP - 228

JO - Revista Ciencias de la Salud

JF - Revista Ciencias de la Salud

SN - 1692-7273

ER -