Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

Carolina Hernández; Carolina Florez; Sergio Castañeda; Nathalia Ballesteros; David Martínez; Adriana Castillo; Marina Muñoz; Sergio Gomez; Angelica Rico; Liseth Pardo; Alberto Paniz-Mondolfi; Juan David Ramírez

doi:10.1002/jmv.27051

Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

Carolina Hernández, Carolina Florez, Sergio Castañeda, Nathalia Ballesteros, David Martínez, Adriana Castillo, Marina Muñoz, Sergio Gomez, Angelica Rico, Liseth Pardo, Alberto Paniz-Mondolfi, Juan David Ramírez

Producción científica: Contribución a una revista › Artículo de Investigación › revisión exhaustiva

11 Citas (Scopus)

Resumen

The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the design and development of multiple reverse-transcription polymerase chain reaction kits aimed to facilitate the rapid scale-up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS-CoV-2 genetic variability and its potential impact in current molecular diagnostic assays.

Idioma original	Inglés estadounidense
Publicación	Journal of Medical Virology
DOI	https://doi.org/10.1002/jmv.27051
Estado	En prensa - 2021

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Virología
Enfermedades infecciosas

ODS de las Naciones Unidas

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Hernández, C., Florez, C., Castañeda, S., Ballesteros, N., Martínez, D., Castillo, A., Muñoz, M., Gomez, S., Rico, A., Pardo, L., Paniz-Mondolfi, A., & Ramírez, J. D. (Aceptado/En prensa). Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia. Journal of Medical Virology. https://doi.org/10.1002/jmv.27051

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abstract = "The ongoing severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has led to the design and development of multiple reverse-transcription polymerase chain reaction kits aimed to facilitate the rapid scale-up of molecular testing for massive screening. We evaluated the diagnostic performance of nine commercial kits, which showed optimal performance and high discriminatory power. However, we observed differences in terms of sensitivity, specificity, and E gene Ct Values and discuss these results in light of the influence of SARS-CoV-2 genetic variability and its potential impact in current molecular diagnostic assays.",

author = "Carolina Hern{\'a}ndez and Carolina Florez and Sergio Casta{\~n}eda and Nathalia Ballesteros and David Mart{\'i}nez and Adriana Castillo and Marina Mu{\~n}oz and Sergio Gomez and Angelica Rico and Liseth Pardo and Alberto Paniz-Mondolfi and Ram{\'i}rez, {Juan David}",

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AU - Florez, Carolina

AU - Castañeda, Sergio

AU - Ballesteros, Nathalia

AU - Martínez, David

AU - Castillo, Adriana

AU - Muñoz, Marina

AU - Gomez, Sergio

AU - Rico, Angelica

AU - Pardo, Liseth

AU - Paniz-Mondolfi, Alberto

AU - Ramírez, Juan David

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Evaluation of the diagnostic performance of nine commercial RT-PCR kits for the detection of SARS-CoV-2 in Colombia

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Áreas temáticas de ASJC Scopus

ODS de las Naciones Unidas

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