Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia

Juan David Ramirez Gonzalez, Giovanny Herrera, Carolina Hernández, Martha Ayala, Carolina Flórez, Aníbal Teherán

Resultado de la investigación: Contribución a RevistaArtículo

6 Citas (Scopus)

Resumen

Background
Leishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.

Methods
Seven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.

Results
The identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.

Conclusions
To our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country.
Idioma originalEnglish (US)
Páginas (desde-hasta)1 - 10
Número de páginas10
PublicaciónPreventive Veterinary Medicine
Volumen10
N.º236
DOI
EstadoPublished - 2017

Huella dactilar

Leishmania guyanensis
Leishmania braziliensis
Multilocus Sequence Typing
Colombia
Leishmania
Leishmaniasis
Diploidy
genetic variation
leishmaniasis
Geographic Mapping
Disease Vectors
diploidy
South America
georeferencing
Genetic Markers
vector-borne diseases
Monoclonal Antibodies
monoclonal antibodies
geographical distribution
Skin

Citar esto

Ramirez Gonzalez, Juan David ; Herrera, Giovanny ; Hernández, Carolina ; Ayala, Martha ; Flórez, Carolina ; Teherán, Aníbal. / Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia. En: Preventive Veterinary Medicine. 2017 ; Vol. 10, N.º 236. pp. 1 - 10.
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title = "Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia",
abstract = "BackgroundLeishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.MethodsSeven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.ResultsThe identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.ConclusionsTo our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country.",
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Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia. / Ramirez Gonzalez, Juan David; Herrera, Giovanny; Hernández, Carolina; Ayala, Martha; Flórez, Carolina; Teherán, Aníbal.

En: Preventive Veterinary Medicine, Vol. 10, N.º 236, 2017, p. 1 - 10.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Evaluation of a Multilocus Sequence Typing (MLST) scheme for Leishmania (Viannia) braziliensis and Leishmania (Viannia) panamensis in Colombia

AU - Ramirez Gonzalez, Juan David

AU - Herrera, Giovanny

AU - Hernández, Carolina

AU - Ayala, Martha

AU - Flórez, Carolina

AU - Teherán, Aníbal

PY - 2017

Y1 - 2017

N2 - BackgroundLeishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.MethodsSeven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.ResultsThe identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.ConclusionsTo our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country.

AB - BackgroundLeishmaniases are parasitic vector-borne diseases affecting more than 12 million people in 98 countries. In Colombia, leishmaniasis is widespread and the most common clinical manifestation is cutaneous, mainly caused by L. panamensis and L. braziliensis. Currently, the genetic diversity of these species in Colombia is unknown. To address this, we applied molecular techniques for their characterization, using multilocus sequence typing (MLST) to explore the genetic variability and phylodynamics of the disease.MethodsSeven previously described genetic markers were selected highlighting the implementation of a mitochondrial marker. Markers were applied to 163 samples from isolates obtained between 1980 and 2001.ResultsThe identification of the samples showed an excellent correlation with typing tests previously applied (MLEE, monoclonal antibodies). Isolates of L. braziliensis showed greater genetic diversity than L. panamensis, and a greater number of diploid sequence types (DSTs). In addition, the geographical distribution of DSTs for each species were obtained through georeferencing maps.ConclusionsTo our knowldge, this study represents the first description of the genetic variability of L. panamensis in Colombia and South America, and is the first to propose a scheme of MLST for epidemiological surveillance of leishmaniasis in the country.

U2 - 10.1186/s13071-017-2175-8

DO - 10.1186/s13071-017-2175-8

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JO - Preventive Veterinary Medicine

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