Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model

Mayra Juliana Laverde-Paz, María Clara Echeverry, Manuel Alfonso Patarroyo, Felio Jesús Bello

Resultado de la investigación: Contribución a RevistaArtículo

1 Cita (Scopus)

Resumen

Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC50) and macrophage cytotoxicity (LC50). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata-derived larval-ES L. panamensis macrophage LC50 was 72.57μg/mL (65.35-80.58μg/mL) and promastigote IC50 was 41.44μg/mL (38.57-44.52μg/mL), compared to 34.93μg/mL (31.65-38.55μg/mL) LC50 and 23.42μg/mL (22.48-24.39μg/mL) IC50 for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10μg/mL L. sericata ES and 5μg/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES.

Idioma originalEnglish (US)
Páginas (desde-hasta)44-50
Número de páginas7
PublicaciónActa Tropica
Volumen177
DOI
EstadoPublished - ene 2018

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title = "Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model",
abstract = "Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC50) and macrophage cytotoxicity (LC50). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata-derived larval-ES L. panamensis macrophage LC50 was 72.57μg/mL (65.35-80.58μg/mL) and promastigote IC50 was 41.44μg/mL (38.57-44.52μg/mL), compared to 34.93μg/mL (31.65-38.55μg/mL) LC50 and 23.42μg/mL (22.48-24.39μg/mL) IC50 for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10μg/mL L. sericata ES and 5μg/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES.",
author = "Laverde-Paz, {Mayra Juliana} and Echeverry, {Mar{\'i}a Clara} and Patarroyo, {Manuel Alfonso} and Bello, {Felio Jes{\'u}s}",
note = "Copyright {\circledC} 2017 Elsevier B.V. All rights reserved.",
year = "2018",
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doi = "10.1016/j.actatropica.2017.09.033",
language = "English (US)",
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Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model. / Laverde-Paz, Mayra Juliana; Echeverry, María Clara; Patarroyo, Manuel Alfonso; Bello, Felio Jesús.

En: Acta Tropica, Vol. 177, 01.2018, p. 44-50.

Resultado de la investigación: Contribución a RevistaArtículo

TY - JOUR

T1 - Evaluating the anti-leishmania activity of Lucilia sericata and Sarconesiopsis magellanica blowfly larval excretions/secretions in an in vitro model

AU - Laverde-Paz, Mayra Juliana

AU - Echeverry, María Clara

AU - Patarroyo, Manuel Alfonso

AU - Bello, Felio Jesús

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2018/1

Y1 - 2018/1

N2 - Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC50) and macrophage cytotoxicity (LC50). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata-derived larval-ES L. panamensis macrophage LC50 was 72.57μg/mL (65.35-80.58μg/mL) and promastigote IC50 was 41.44μg/mL (38.57-44.52μg/mL), compared to 34.93μg/mL (31.65-38.55μg/mL) LC50 and 23.42μg/mL (22.48-24.39μg/mL) IC50 for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10μg/mL L. sericata ES and 5μg/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES.

AB - Leishmaniasis is a vector-borne disease caused by infection by parasites from the genus Leishmania. Clinical manifestations can be visceral or cutaneous, the latter mainly being chronic ulcers. This work was aimed at evaluating Calliphoridae Lucilia sericata- and Sarconesiopsis magellanica-derived larval excretions and secretions' (ES) in vitro anti-leishmanial activity against Leishmania panamensis. Different larval-ES concentrations from both blowfly species were tested against either L. panamensis promastigotes or intracellular amastigotes using U937-macrophages as host cells. The Alamar Blue method was used for assessing parasite half maximal inhibitory concentration (IC50) and macrophage cytotoxicity (LC50). The effect of larval-ES on L. panamensis intracellular parasite forms was evaluated by calculating the percentage of infected macrophages, parasite load and toxicity. L. sericata-derived larval-ES L. panamensis macrophage LC50 was 72.57μg/mL (65.35-80.58μg/mL) and promastigote IC50 was 41.44μg/mL (38.57-44.52μg/mL), compared to 34.93μg/mL (31.65-38.55μg/mL) LC50 and 23.42μg/mL (22.48-24.39μg/mL) IC50 for S. magellanica. Microscope evaluation of intracellular parasite forms showed that treatment with 10μg/mL L. sericata ES and 5μg/mL S. magellanica ES led to a decrease in the percentage of infected macrophages and the amount of intracellular amastigotes. This study produced in vitro evidence of the antileishmanial activity of larval ES from both blowfly species on different parasitic stages and showed that the parasite was more susceptible to the ES than it's host cells. The antileishmanial effect on L. panamensis was more evident from S. magellanica ES.

U2 - 10.1016/j.actatropica.2017.09.033

DO - 10.1016/j.actatropica.2017.09.033

M3 - Article

VL - 177

SP - 44

EP - 50

JO - Acta Tropica

JF - Acta Tropica

SN - 0001-706X

ER -