Enterococcus faecalis internalization in human umbilical vein endothelial cells (HUVEC).

Diana Millán, Carlos Chiriboga, Manuel A. Patarroyo, Marta R. Fontanilla

    Resultado de la investigación: Contribución a RevistaArtículo

    4 Citas (Scopus)

    Resumen

    Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive bacteria-cell interactions; however, few studies have been aimed at establishing the relationship of E. faecalis with endothelial cells. The aggregation substance (AS) family of adhesins represents an E. faecalis virulence factor which has been implicated in endocarditis severity and bacterial persistence. The Asc10 protein (a member of this family) promotes bacterium-bacterium aggregation and bacterium-host cell binding. Evaluating Asc10 role in bacterial internalization by cultured enterocytes has shown that this adhesin facilitates E. faecalis endocytosis by HT-29 cells. A few eukaryotic cell structural components, such as cytoskeletal proteins, have been involved in E. faecalis entry into cell-lines; it is thus relevant to determine whether Asc10, as well as microtubules and actin microfilaments, play a role in E. faecalis internalization by cultured endothelial cells. The role of Asc10 and cytoskeleton proteins in E. faecalis ability to enter HUVEC was assessed in the present study, as well as cell apoptosis induction by enterococcal internalization by HUVEC; the data indicated increased cell apoptosis and that cytoskeleton components were partially involved in E. faecalis entry to endothelial cells, thereby suggesting that E. faecalis Asc10 protein would not be a critical factor for bacterial entry to cultured HUVEC. Copyright © 2012 Elsevier Ltd. All rights reserved.
    Idioma originalEnglish (US)
    Páginas (desde-hasta)62-69
    Número de páginas8
    PublicaciónMicrobial Pathogenesis
    DOI
    EstadoPublished - abr 1 2013

    Huella dactilar

    Enterococcus faecalis
    Human Umbilical Vein Endothelial Cells
    Endothelial Cells
    Cytoskeleton
    Bacteria
    Cell Communication
    Apoptosis
    Bacterial Endocarditis
    HT29 Cells
    Aptitude
    Cytoskeletal Proteins
    Enterocytes
    Enterococcus
    Gram-Positive Bacteria
    Eukaryotic Cells
    Virulence Factors
    Cellular Structures
    Endocytosis
    Actin Cytoskeleton
    Microtubules

    Citar esto

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    title = "Enterococcus faecalis internalization in human umbilical vein endothelial cells (HUVEC).",
    abstract = "Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive bacteria-cell interactions; however, few studies have been aimed at establishing the relationship of E. faecalis with endothelial cells. The aggregation substance (AS) family of adhesins represents an E. faecalis virulence factor which has been implicated in endocarditis severity and bacterial persistence. The Asc10 protein (a member of this family) promotes bacterium-bacterium aggregation and bacterium-host cell binding. Evaluating Asc10 role in bacterial internalization by cultured enterocytes has shown that this adhesin facilitates E. faecalis endocytosis by HT-29 cells. A few eukaryotic cell structural components, such as cytoskeletal proteins, have been involved in E. faecalis entry into cell-lines; it is thus relevant to determine whether Asc10, as well as microtubules and actin microfilaments, play a role in E. faecalis internalization by cultured endothelial cells. The role of Asc10 and cytoskeleton proteins in E. faecalis ability to enter HUVEC was assessed in the present study, as well as cell apoptosis induction by enterococcal internalization by HUVEC; the data indicated increased cell apoptosis and that cytoskeleton components were partially involved in E. faecalis entry to endothelial cells, thereby suggesting that E. faecalis Asc10 protein would not be a critical factor for bacterial entry to cultured HUVEC. Copyright {\circledC} 2012 Elsevier Ltd. All rights reserved.",
    author = "Diana Mill{\'a}n and Carlos Chiriboga and Patarroyo, {Manuel A.} and Fontanilla, {Marta R.}",
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    Enterococcus faecalis internalization in human umbilical vein endothelial cells (HUVEC). / Millán, Diana; Chiriboga, Carlos; Patarroyo, Manuel A.; Fontanilla, Marta R.

    En: Microbial Pathogenesis, 01.04.2013, p. 62-69.

    Resultado de la investigación: Contribución a RevistaArtículo

    TY - JOUR

    T1 - Enterococcus faecalis internalization in human umbilical vein endothelial cells (HUVEC).

    AU - Millán, Diana

    AU - Chiriboga, Carlos

    AU - Patarroyo, Manuel A.

    AU - Fontanilla, Marta R.

    PY - 2013/4/1

    Y1 - 2013/4/1

    N2 - Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive bacteria-cell interactions; however, few studies have been aimed at establishing the relationship of E. faecalis with endothelial cells. The aggregation substance (AS) family of adhesins represents an E. faecalis virulence factor which has been implicated in endocarditis severity and bacterial persistence. The Asc10 protein (a member of this family) promotes bacterium-bacterium aggregation and bacterium-host cell binding. Evaluating Asc10 role in bacterial internalization by cultured enterocytes has shown that this adhesin facilitates E. faecalis endocytosis by HT-29 cells. A few eukaryotic cell structural components, such as cytoskeletal proteins, have been involved in E. faecalis entry into cell-lines; it is thus relevant to determine whether Asc10, as well as microtubules and actin microfilaments, play a role in E. faecalis internalization by cultured endothelial cells. The role of Asc10 and cytoskeleton proteins in E. faecalis ability to enter HUVEC was assessed in the present study, as well as cell apoptosis induction by enterococcal internalization by HUVEC; the data indicated increased cell apoptosis and that cytoskeleton components were partially involved in E. faecalis entry to endothelial cells, thereby suggesting that E. faecalis Asc10 protein would not be a critical factor for bacterial entry to cultured HUVEC. Copyright © 2012 Elsevier Ltd. All rights reserved.

    AB - Initial Enterococcus faecalis-endothelial cell molecular interactions which lead to enterococci associating in the host endothelial tissue, colonizing it and proliferating there can be assessed using in vitro models. Cultured human umbilical vein endothelial cells (HUVEC) have been used to study other Gram-positive bacteria-cell interactions; however, few studies have been aimed at establishing the relationship of E. faecalis with endothelial cells. The aggregation substance (AS) family of adhesins represents an E. faecalis virulence factor which has been implicated in endocarditis severity and bacterial persistence. The Asc10 protein (a member of this family) promotes bacterium-bacterium aggregation and bacterium-host cell binding. Evaluating Asc10 role in bacterial internalization by cultured enterocytes has shown that this adhesin facilitates E. faecalis endocytosis by HT-29 cells. A few eukaryotic cell structural components, such as cytoskeletal proteins, have been involved in E. faecalis entry into cell-lines; it is thus relevant to determine whether Asc10, as well as microtubules and actin microfilaments, play a role in E. faecalis internalization by cultured endothelial cells. The role of Asc10 and cytoskeleton proteins in E. faecalis ability to enter HUVEC was assessed in the present study, as well as cell apoptosis induction by enterococcal internalization by HUVEC; the data indicated increased cell apoptosis and that cytoskeleton components were partially involved in E. faecalis entry to endothelial cells, thereby suggesting that E. faecalis Asc10 protein would not be a critical factor for bacterial entry to cultured HUVEC. Copyright © 2012 Elsevier Ltd. All rights reserved.

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    DO - 10.1016/j.micpath.2012.11.007

    M3 - Article

    SP - 62

    EP - 69

    JO - Microbial Pathogenesis

    JF - Microbial Pathogenesis

    SN - 0882-4010

    ER -