Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta

Robinson Ramírez-Vélez, Juanita Bustamante, Analia Czerniczyniec, Ana C. Aguilar De Plata, Silvia Lores-Arnaiz

Resultado de la investigación: Contribución a RevistaArtículo

17 Citas (Scopus)

Resumen

Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1-3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. © 2013 Ramírez-Vélez et al.
Idioma originalEnglish (US)
Páginas (desde-hasta)E80225
PublicaciónPLoS One
Volumen8
N.º11
DOI
EstadoPublished - nov 14 2013
Publicado de forma externa

Huella dactilar

placenta
Metabolism
Placenta
nitric oxide
Nitric Oxide
exercise
Exercise
Oxygen
oxygen
metabolism
strength training
Superoxides
Hydrogen Peroxide
pregnancy
Tissue
Mitochondria
Centrifugation
cytosol
superoxide anion
Pregnancy

Citar esto

Ramírez-Vélez, R., Bustamante, J., Czerniczyniec, A., Aguilar De Plata, A. C., & Lores-Arnaiz, S. (2013). Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta. PLoS One, 8(11), E80225. https://doi.org/10.1371/journal.pone.0080225
Ramírez-Vélez, Robinson ; Bustamante, Juanita ; Czerniczyniec, Analia ; Aguilar De Plata, Ana C. ; Lores-Arnaiz, Silvia. / Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta. En: PLoS One. 2013 ; Vol. 8, N.º 11. pp. E80225.
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abstract = "Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75{\%} of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1-3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8{\%} and 37{\%} respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. {\circledC} 2013 Ram{\'i}rez-V{\'e}lez et al.",
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Ramírez-Vélez, R, Bustamante, J, Czerniczyniec, A, Aguilar De Plata, AC & Lores-Arnaiz, S 2013, 'Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta', PLoS One, vol. 8, n.º 11, pp. E80225. https://doi.org/10.1371/journal.pone.0080225

Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta. / Ramírez-Vélez, Robinson; Bustamante, Juanita; Czerniczyniec, Analia; Aguilar De Plata, Ana C.; Lores-Arnaiz, Silvia.

En: PLoS One, Vol. 8, N.º 11, 14.11.2013, p. E80225.

Resultado de la investigación: Contribución a RevistaArtículo

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T1 - Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta

AU - Ramírez-Vélez, Robinson

AU - Bustamante, Juanita

AU - Czerniczyniec, Analia

AU - Aguilar De Plata, Ana C.

AU - Lores-Arnaiz, Silvia

PY - 2013/11/14

Y1 - 2013/11/14

N2 - Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1-3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. © 2013 Ramírez-Vélez et al.

AB - Objective: To determine the effects of combined aerobic and resistance exercise training during the second half of pregnancy on endothelial NOS expression (eNOS), nitric oxide (NO) production and oxygen metabolism in human placenta. Methods: The study included 20 nulliparous in gestational week 16-20, attending prenatal care at three tertiary hospitals in Colombia who were randomly assigned into one of two groups: The exercise group (n = 10) took part in an exercise session three times a week for 12 weeks which consisted of: aerobic exercise at an intensity of 55-75% of their maximum heart rate for 60 min and 25 mins. Resistance exercise included 5 exercise groups circuit training (50 repetitions of each) using barbells (1-3 kg/exercise) and low-to-medium resistance bands. The control group (n = 10) undertook their usual physical activity. Mitochondrial and cytosol fractions were isolated from human placental tissue by differential centrifugation. A spectrophotometric assay was used to measure NO production in cytosolic samples from placental tissue and Western Blot technique to determine eNOS expression. Mitochondrial superoxide levels and hydrogen peroxide were measured to determine oxygen metabolism. Results: Combined aerobic and resistance exercise training during pregnancy leads to a 2-fold increase in eNOS expression and 4-fold increase in NO production in placental cytosol (p = 0.05). Mitochondrial superoxide levels and hydrogen peroxide production rate were decreased by 8% and 37% respectively in the placental mitochondria of exercising women (p = 0.05). Conclusion: Regular exercise training during the second half of pregnancy increases eNOS expression and NO production and decreases reactive oxygen species generation in human placenta. Collectively, these data demonstrate that chronic exercise increases eNOS/NO production, presumably by increasing endothelial shear stress. This adaptation may contribute to the beneficial effects of exercise on the vascular and antioxidant system and in turn reduce the risk of preeclampsia, diabetes or hypertension during pregnancy. © 2013 Ramírez-Vélez et al.

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DO - 10.1371/journal.pone.0080225

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Ramírez-Vélez R, Bustamante J, Czerniczyniec A, Aguilar De Plata AC, Lores-Arnaiz S. Effect of exercise training on enos expression, NO production and oxygen metabolism in human placenta. PLoS One. 2013 nov 14;8(11):E80225. https://doi.org/10.1371/journal.pone.0080225